PAPOLG (NM_022894) Human Tagged Lenti ORF Clone
$974.00
3 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PAPOLG |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_022894 |
| ORF Size | 2208 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_022894.2 |
| RefSeq Size | 7386 bp |
| RefSeq ORF | 2211 bp |
| Locus ID | 64895 |
| UniProt ID | Q9BWT3 |
| Cytogenetics | 2p16.1 |
| Domains | NTP_transf_2, PAP_central, PAP_RNA-bind |
| Protein Families | Transcription Factors |
| Protein Pathways | RNA degradation |
| MW | 82.8 kDa |
| Summary | This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_022894" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC213918 | PAPOLG (Myc-DDK-tagged)-Human poly(A) polymerase gamma (PAPOLG) | 10 ug |
$674.00
|
|
| RC213918L3 | Lenti ORF clone of Human poly(A) polymerase gamma (PAPOLG), Myc-DDK-tagged | 10 ug |
$974.00
|
|
| RG213918 | PAPOLG (tGFP-tagged) - Human poly(A) polymerase gamma (PAPOLG) | 10 ug |
$789.00
$874.00
|
|
| SC310141 | PAPOLG (untagged)-Human poly(A) polymerase gamma (PAPOLG) | 10 ug |
$742.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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