EIF4G1 (NM_004953) Human Tagged ORF Clone

SKU RG212877

EIF4G1 (tGFP-tagged) - Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 5

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: EIF4G1
• Synonyms: EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212877 representing NM_004953; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCTGGGGCCCGCACTGCCTCCACACCCACCCCTCCCCAGACGGGAGGCGGTCTGGAGCCTCAAGCTA; ATGGGGAGACGCCCCAGGTTGCTGTCATTGTCCGGCCAGATGACCGGTCACAGGGAGCAATCATTGCTGA; CCGGCCAGGGCTGCCTGGCCCAGAGCATAGCCCTTCAGAATCCCAGCCTTCGTCGCCTTCTCCGACCCCA; TCACCATCCCCAGTCTTGGAACCGGGGTCTGAGCCTAATCTCGCAGTCCTCTCTATTCCTGGGGACACTA; TGACAACTATACAAATGTCTGTAGAAGAATCAACCCCCATCTCCCGTGAAACTGGGGAGCCATATCGCCT; CTCTCCAGAACCCACTCCTCTCGCCGAACCCATACTGGAAGTAGAAGTGACACTTAGCAAACCGGTTCCA; GAATCTGAGTTTTCTTCCAGTCCTCTCCAGGCTCCCACCCCTTTGGCATCTCACACAGTGGAAATTCATG; AGCCTAATGGCATGGTCCCATCTGAAGATCTGGAACCAGAGGTGGAGTCAAGCCCAGAGCTTGCTCCTCC; CCCAGCTTGCCCCTCCGAATCCCCTGTGCCCATTGCTCCAACTGCCCAACCTGAGGAACTGCTCAACGGA; GCCCCCTCGCCACCAGCTGTGGACTTAAGCCCAGTCAGTGAGCCAGAGGAGCAGGCCAAGGAGGTGACAG; CATCAATGGCGCCCCCCACCATCCCCTCTGCTACTCCAGCTACGGCTCCTTCAGCTACTTCCCCAGCTCA; GGAGGAGGAAATGGAAGAAGAAGAAGAAGAGGAAGAAGGAGAAGCAGGAGAAGCAGGAGAAGCTGAGAGT; GAGAAAGGAGGAGAGGAACTGCTCCCCCCAGAGAGTACCCCTATTCCAGCCAACTTGTCTCAGAATTTGG; AGGCAGCAGCAGCCACTCAAGTGGCAGTATCTGTGCCAAAGAGGAGACGGAAAATTAAGGAGCTAAATAA; GAAGGAGGCTGTTGGAGACCTTCTGGATGCCTTCAAGGAGGCGAACCCGGCAGTACCAGAGGTGGAAAAT; CAGCCTCCTGCAGGCAGCAATCCAGGCCCAGAGTCTGAGGGCAGTGGTGTGCCCCCACGTCCTGAGGAAG; CAGATGAGACCTGGGACTCAAAGGAAGACAAAATTCACAATGCTGAGAACATCCAGCCCGGGGAACAGAA; GTATGAATATAAGTCAGATCAGTGGAAGCCTCTAAACCTAGAGGAGAAAAAACGTTACGACCGTGAGTTC; CTGCTTGGTTTTCAGTTCATCTTTGCCAGTATGCAGAAGCCAGAGGGATTGCCACATATCAGTGACGTGG; TGCTGGACAAGGCCAATAAAACACCACTGCGGCCACTGGATCCCACTAGACTACAAGGCATAAATTGTGG; CCCAGACTTCACTCCATCCTTTGCCAACCTTGGCCGGACAACCCTTAGCACCCGTGGGCCCCCAAGGGGT; GGGCCAGGTGGGGAGCTGCCCCGTGGGCCGGCTGGCCTGGGACCCCGGCGCTCTCAGCAGGGACCCCGAA; AAGAACCACGCAAGATCATTGCCACAGTGTTAATGACCGAAGATATAAAACTGAACAAAGCAGAGAAAGC; CTGGAAACCCAGCAGCAAGCGGACGGCGGCTGATAAGGATCGAGGGGAAGAAGATGCTGATGGCAGCAAA; ACCCAGGACCTATTCCGCAGGGTGCGCTCCATCCTGAATAAACTGACACCCCAGATGTTCCAGCAGCTGA; TGAAGCAAGTGACGCAGCTGGCCATCGACACCGAGGAACGCCTCAAAGGGGTCATTGACCTCATTTTTGA; GAAGGCCATTTCAGAGCCCAACTTCTCTGTGGCCTATGCCAACATGTGCCGCTGCCTCATGGCGCTGAAA; GTGCCCACTACGGAAAAGCCAACAGTGACTGTGAACTTCCGAAAGCTGTTGTTGAATCGATGTCAGAAGG; AGTTTGAGAAAGACAAAGATGATGATGAGGTTTTTGAGAAGAAGCAAAAAGAGATGGATGAAGCTGCTAC; GGCAGAGGAACGAGGACGCCTGAAGGAAGAGCTGGAAGAGGCTCGGGACATAGCCCGGCGGCGCTCTTTA; GGGAATATCAAGTTTATTGGAGAGTTGTTCAAACTGAAGATGTTAACAGAGGCAATAATGCATGACTGTG; TGGTCAAACTGCTTAAGAACCATGATGAAGAGTCCCTTGAGTGCCTTTGTCGTCTGCTCACCACCATTGG; CAAAGACCTGGACTTTGAAAAAGCCAAGCCCCGAATGGATCAGTATTTCAACCAGATGGAAAAAATCATT; AAAGAAAAGAAGACGTCATCCCGCATCCGCTTTATGCTGCAGGACGTGCTGGATCTGCGAGGGAGCAATT; GGGTGCCACGCCGAGGGGATCAGGGTCCCAAGACCATTGACCAGATCCATAAGGAGGCTGAGATGGAAGA; ACATCGAGAGCACATCAAAGTGCAGCAGCTCATGGCCAAGGGCAGTGACAAGCGTCGGGGCGGTCCTCCA; GGCCCTCCCATCAGCCGTGGACTTCCCCTTGTGGATGATGGTGGCTGGAACACAGTTCCCATCAGCAAAG; GTAGCCGCCCCATTGACACCTCACGACTCACCAAGATCACCAAGCCTGGCTCCATCGATTCTAACAACCA; GCTCTTTGCACCTGGAGGGCGACTGAGCTGGGGCAAGGGCAGCAGCGGAGGCTCAGGAGCCAAGCCCTCA; GACGCAGCATCAGAAGCTGCTCGCCCAGCTACTAGTACTTTGAATCGCTTCTCAGCCCTTCAACAAGCGG; TACCCACAGAAAGCACAGATAATAGACGTGTGGTGCAGAGGAGTAGCTTGAGCCGAGAACGAGGCGAGAA; AGCTGGAGACCGAGGAGACCGCCTAGAGCGGAGTGAACGGGGAGGGGACCGTGGGGACCGGCTTGATCGT; GCGCGGACACCTGCTACCAAGCGGAGCTTCAGCAAGGAAGTGGAGGAGCGGAGTAGAGAACGGCCCTCCC; AGCCTGAGGGGCTGCGCAAGGCAGCTAGCCTCACGGAGGATCGGGACCGTGGGCGGGATGCCGTGAAGCG; AGAAGCTGCCCTACCCCCAGTGAGCCCCCTGAAGGCGGCTCTCTCTGAGGAGGAGTTAGAGAAGAAATCC; AAGGCTATCATTGAGGAATATCTCCATCTCAATGACATGAAAGAGGCAGTCCAGTGCGTGCAGGAGCTGG; CCTCACCCTCCTTGCTCTTCATCTTTGTACGGCATGGTGTCGAGTCTACGCTGGAGCGCAGTGCCATTGC; TCGTGAGCATATGGGGCAGCTGCTGCACCAGCTGCTCTGTGCTGGGCATCTGTCTACTGCTCAGTACTAC; CAAGGGTTGTATGAAATCTTGGAATTGGCTGAGGACATGGAAATTGACATCCCCCACGTGTGGCTCTACC; TAGCGGAACTGGTAACACCCATTCTGCAGGAAGGTGGGGTGCCCATGGGGGAGCTGTTCAGGGAGATTAC; AAAGCCTCTGAGACCGTTGGGCAAAGCTGCTTCCCTGTTGCTGGAGATCCTGGGCCTCCTGTGCAAAAGC; ATGGGTCCTAAAAAGGTGGGGACGCTGTGGCGAGAAGCCGGGCTTAGCTGGAAGGAATTTCTACCTGAAG; GCCAGGACATTGGTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCGGAAGCCCC; TGGCCAGAGGGCACTCCCCTCCGAGGAGCTGAACAGGCAGCTGGAGAAGCTGCTGAAGGAGGGCAGCAGT; AACCAGCGGGTGTTCGACTGGATAGAGGCCAACCTGAGTGAGCAGCAGATAGTATCCAACACGTTAGTTC; GAGCCCTCATGACGGCTGTCTGCTATTCTGCAATTATTTTTGAGACTCCCCTCCGAGTGGACGTTGCAGT; GCTGAAAGCGCGAGCGAAGCTGCTGCAGAAATACCTGTGTGACGAGCAGAAGGAGCTACAGGCGCTCTAC; GCCCTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTCCCAACCTGCTGCGGATGTTCTTTGACGCACTGT; ATGACGAGGACGTGGTGAAGGAGGATGCCTTCTACAGTTGGGAGAGTAGCAAGGACCCCGCTGAGCAGCA; GGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCCTTCTTCAAGTGGCTCCGTGAAGCAGAGGAGGAGTCT; GACCACAAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212877 representing NM_004953; Red=Cloning site Green=Tags(s); ; MSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTP; SPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVP; ESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNG; APSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAES; EKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVEN; QPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREF; LLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRG; GPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSK; TQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALK; VPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSL; GNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKII; KEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPP; GPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPS; DAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDR; ARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKS; KAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYY; QGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKS; MGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSS; NQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALY; ALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEES; DHN; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004953
• ORF Size: 4209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004953.3
• RefSeq Size: 5045 bp
• RefSeq ORF: 4215 bp
• Locus ID: 1981
• UniProt ID: Q04637
• Cytogenetics: 3q27.1
• Domains: eIF5C, MA3, MIF4G
• Protein Pathways: Viral myocarditis
• Summary: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]