EIF4G1 (NM_004953) Human Untagged Clone
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | EIF4G1 |
| Synonyms | EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_004953 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004953.2, NP_004944.2 |
| RefSeq Size | 5045 bp |
| RefSeq ORF | 4212 bp |
| Locus ID | 1981 |
| UniProt ID | Q04637 |
| Cytogenetics | 3q27.1 |
| Domains | eIF5C, MA3, MIF4G |
| Protein Pathways | Viral myocarditis |
| Summary | The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. provided by RefSeq, Aug 2010 Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The resulting isoform (4) is shorter at the N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004953" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC212877 | EIF4G1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 5 | 10 ug |
$1,200.00
|
|
| RC212877L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 5, Myc-DDK-tagged | 10 ug |
$1,500.00
|
|
| RC212877L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 5, mGFP tagged | 10 ug |
$1,500.00
|
|
| RC212877L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 5, Myc-DDK-tagged | 10 ug |
$1,500.00
|
|
| RC212877L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 5, mGFP tagged | 10 ug |
$1,500.00
|
|
| RG212877 | EIF4G1 (tGFP-tagged) - Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 5 | 10 ug |
$1,400.00
|
|
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