PHC2 (NM_004427) Human Tagged ORF Clone

SKU RG212697

PHC2 (tGFP-tagged) - Human polyhomeotic homolog 2 (Drosophila) (PHC2), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PHC2
• Synonyms: EDR2; HPH2; PH2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212697 representing NM_004427; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACCTCAGGGAACGGAAACTCTGCCTCCAGCATCGCCGGCACTGCCCCCCAGAATGGTGAGAATAAAC; CACCACAGGCCATTGTGAAACCCCAAATCCTGACGCATGTTATCGAAGGGTTTGTGATCCAGGAGGGGGC; GGAGCCTTTCCCGGTGGGACGCTCGTCCCTGCTGGTGGGGAATCTCAAGAAGAAGTATGCACAGGGGTTC; CTGCCTGAGAAACTTCCACAGCAGGATCACACCACCACCACTGACTCGGAGATGGAGGAGCCCTATCTGC; AAGAATCCAAAGAGGAGGGTGCTCCCCTCAAACTCAAGTGTGAGCTCTGTGGCCGGGTGGACTTTGCCTA; TAAGTTCAAGCGTTCCAAGCGCTTCTGTTCCATGGCTTGTGCAAAGAGGTACAACGTGGGATGCACCAAA; CGGGTGGGACTTTTCCACTCAGACCGGAGCAAGCTGCAGAAGGCAGGAGCTGCGACCCACAACCGCCGTC; GGGCCAGCAAAGCCAGTCTGCCACCACTTACCAAGGATACCAAGAAGCAGCCAACAGGCACTGTGCCCCT; TTCGGTTACTGCTGCTTTGCAGCTAACACACAGCCAGGAAGACTCCAGCCGTTGCTCAGATAACTCAAGC; TATGAGGAACCCTTGTCACCCATCTCAGCCAGCTCATCTACTTCCCGCCGGCGACAAGGCCAGCGGGACC; TGGAGCTCCCCGACATGCATATGCGGGACCTGGTGGGCATGGGACACCACTTCCTGCCAAGTGAGCCCAC; CAAGTGGAATGTAGAAGACGTCTACGAATTCATCCGCTCTCTGCCAGGCTGCCAGGAGATAGCAGAGGAA; TTCCGTGCCCAGGAAATCGACGGGCAAGCCCTGCTGCTGCTCAAGGAGGACCACCTGATGAGCGCCATGA; ACATCAAGCTGGGGCCCGCCCTGAAGATCTACGCCCGCATCAGCATGCTCAAGGACTCC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212697 representing NM_004427; Red=Cloning site Green=Tags(s); ; MTSGNGNSASSIAGTAPQNGENKPPQAIVKPQILTHVIEGFVIQEGAEPFPVGRSSLLVGNLKKKYAQGF; LPEKLPQQDHTTTTDSEMEEPYLQESKEEGAPLKLKCELCGRVDFAYKFKRSKRFCSMACAKRYNVGCTK; RVGLFHSDRSKLQKAGAATHNRRRASKASLPPLTKDTKKQPTGTVPLSVTAALQLTHSQEDSSRCSDNSS; YEEPLSPISASSSTSRRRQGQRDLELPDMHMRDLVGMGHHFLPSEPTKWNVEDVYEFIRSLPGCQEIAEE; FRAQEIDGQALLLLKEDHLMSAMNIKLGPALKIYARISMLKDS; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004427
• ORF Size: 969 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004427.4
• RefSeq Size: 2597 bp
• RefSeq ORF: 972 bp
• Locus ID: 1912
• UniProt ID: Q8IXK0
• Cytogenetics: 1p35.1
• Summary: In Drosophila melanogaster, the 'Polycomb' group (PcG) of genes are part of a cellular memory system that is responsible for the stable inheritance of gene activity. PcG proteins form a large multimeric, chromatin-associated protein complex. The protein encoded by this gene has homology to the Drosophila PcG protein 'polyhomeotic' (Ph) and is known to heterodimerize with EDR1 and colocalize with BMI1 in interphase nuclei of human cells. The specific function in human cells has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]