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Apolipoprotein L 1 (APOL1) (NM_003661) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG211114
APOL1 (tGFP-tagged) - Human apolipoprotein L, 1 (APOL1), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $886.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Apolipoprotein L 1
Synonyms APO-L; APOL; APOL-I; FSGS4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG211114 representing NM_003661
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATT
CGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC

ATGGAGGGAGCTGCTTTGCTGAGAGTCTCTGTCCTCTGCATCTGGATGAGTGCACTTTTC
CTTGGTGTGGGAGTGAGGGCAGAGGAAGCTGGAGCGAGGGTGCAACAAAACGTTCCAAGT
GGGACAGATACTGGAGATCCTCAAAGTAAGCCCCTCGGTGACTGGGCTGCTGGCACCATG
GACCCAGAGAGCAGTATCTTTATTGAGGATGCCATTAAGTATTTCAAGGAAAAAGTGAGC
ACACAGAATCTGCTACTCCTGCTGACTGATAATGAGGCCTGGAACGGATTCGTGGCTGCT
GCTGAACTGCCCAGGAATGAGGCAGATGAGCTCCGTAAAGCTCTGGACAACCTTGCAAGA
CAAATGATCATGAAAGACAAAAACTGGCACGATAAAGGCCAGCAGTACAGAAACTGGTTT
CTGAAAGAGTTTCCTCGGTTGAAAAGTGAGCTTGAGGATAACATAAGAAGGCTCCGTGCC
CTTGCAGATGGGGTTCAGAAGGTCCACAAAGGCACCACCATCGCCAATGTGGTGTCTGGC
TCTCTCAGCATTTCCTCTGGCATCCTGACCCTCGTCGGCATGGGTCTGGCACCCTTCACA
GAGGGAGGCAGCCTTGTACTCTTGGAACCTGGGATGGAGTTGGGAATCACAGCCGCTTTG
ACCGGGATTACCAGCAGTACCATGGACTACGGAAAGAAGTGGTGGACACAAGCCCAAGCC
CACGACCTGGTCATCAAAAGCCTTGACAAATTGAAGGAGGTGAGGGAGTTTTTGGGTGAG
AACATATCCAACTTTCTTTCCTTAGCTGGCAATACTTACCAACTCACACGAGGCATTGGG
AAGGACATCCGTGCCCTCAGACGAGCCAGAGCCAATCTTCAGTCAGTACCGCATGCCTCA
GCCTCACGCCCCCGGGTCACTGAGCCAATCTCAGCTGAAAGCGGTGAACAGGTGGAGAGG
GTTAATGAACCCAGCATCCTGGAAATGAGCAGAGGAGTCAAGCTCACGGATGTGGCCCCT
GTAAGCTTCTTTCTTGTGCTGGATGTAGTCTACCTCGTGTACGAATCAAAGCACTTACAT
GAGGGGGCAAAGTCAGAGACAGCTGAGGAGCTGAAGAAGGTGGCTCAGGAGCTGGAGGAG
AAGCTAAACATTCTCAACAATAATTATAAGATTCTGCAGGCGGACCAAGAACTG

GACGCGTACGCGGCCGCTCGAGATGGAGAGCGACGAGAGCGGCCTGCCCGCCATGGAGATCGAGTGCCGCATCACCGGCACC
CTGAACGGCGTGGAGTTCGAGCT
Protein Sequence
>RG211114 protein sequence
Blue=ORF Red=Cloning site Green=Tag(s)

MEGAALLRVSVLCIWMSALFLGVGVRAEEAGARVQQNVPSGTDTGDPQSKPLGDWAAGTM
DPESSIFIEDAIKYFKEKVSTQNLLLLLTDNEAWNGFVAAAELPRNEADELRKALDNLAR
QMIMKDKNWHDKGQQYRNWFLKEFPRLKSELEDNIRRLRALADGVQKVHKGTTIANVVSG
SLSISSGILTLVGMGLAPFTEGGSLVLLEPGMELGITAALTGITSSTMDYGKKWWTQAQA
HDLVIKSLDKLKEVREFLGENISNFLSLAGNTYQLTRGIGKDIRALRRARANLQSVPHAS
ASRPRVTEPISAESGEQVERVNEPSILEMSRGVKLTDVAPVSFFLVLDVVYLVYESKHLH
EGAKSETAEELKKVAQELEEKLNILNNNYKILQADQEL

DAYAAARDGERRERPARHGDRVPHHRHPERRGVRAGGRRRGHPRAGPHDQQDEEHQRRPD
LQPLPAEPRDGLRLLPLRHLPQRLRE
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003661
ORF Size 1194 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003661.4
RefSeq Size 2856 bp
RefSeq ORF 1197 bp
Locus ID 8542
UniProt ID O14791
Cytogenetics 22q12.3
Protein Families Secreted Protein, Transmembrane
Summary This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Nov 2008
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Documents
Resources
"NM_003661" in other vectors (6)
SKU Description Size Price
RC211114 APOL1 (Myc-DDK-tagged)-Human apolipoprotein L, 1 (APOL1), transcript variant 1 10 ug
$686.00
RC211114L1 Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 1, Myc-DDK-tagged 10 ug
$986.00
RC211114L2 Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 1, mGFP tagged 10 ug
$986.00
RC211114L3 Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 1, Myc-DDK-tagged 10 ug
$986.00
RC211114L4 Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 1, mGFP tagged 10 ug
$986.00
SC109941 APOL1 (untagged)-Human apolipoprotein L, 1 (APOL1), transcript variant 1 10 ug
$457.00

Plasmid Map for RG211114

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG211114
Circular map for RG211114

file-alt Citations

Regulation of Fluid-phase Uptake in Podocytes by Albumin-associated Lipids
Chung
Thesis (2014)
Snippet: Mammalian expression vectors (pCMV6-AC-GFP) containing the cDNAs of ApoL1 – 4 and a C-terminal GFP tag were purchased from Origene (Rockville, MD). G1 (S342G, I384M) and G2 (deletion of N388 and Y389) variants of ApoL1 were generated by site-directed mutagenesis from the pCMV-ApoL1-GFP construct using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA)
Showing 1-1 of 1 papers.
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