Apolipoprotein L 1 (APOL1) (NM_003661) Human Tagged ORF Clone
- TrueORF®
SKU
RC211114
APOL1 (Myc-DDK-tagged)-Human apolipoprotein L, 1 (APOL1), transcript variant 1
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Apolipoprotein L 1 |
| Synonyms | APO-L; APOL; APOL-I; FSGS4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003661 |
| ORF Size | 1194 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003661.4 |
| RefSeq Size | 2795 bp |
| RefSeq ORF | 1197 bp |
| Locus ID | 8542 |
| UniProt ID | O14791 |
| Cytogenetics | 22q12.3 |
| Protein Families | Secreted Protein, Transmembrane |
| MW | 44 kDa |
| Summary | This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Nov 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003661" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC211114L1 | Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC211114L2 | Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RC211114L3 | Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC211114L4 | Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RG211114 | APOL1 (tGFP-tagged) - Human apolipoprotein L, 1 (APOL1), transcript variant 1 | 10 ug |
$886.00
|
|
| SC109941 | APOL1 (untagged)-Human apolipoprotein L, 1 (APOL1), transcript variant 1 | 10 ug |
$457.00
|
|
Citations
Recruitment of APOL1 kidney disease risk variants to lipid droplets attenuates cell toxicity
Chun, Zhang, Wilkins et al
PNAS (2019) 116 (9), 3712-3721 DOI: 10.1073/pnas.1820414116
Chun, Zhang, Wilkins et al
PNAS (2019) 116 (9), 3712-3721 DOI: 10.1073/pnas.1820414116
APOL1-G1 in Nephrocytes Induces Hypertrophy and Accelerates Cell Death
Fu, Zhu, Richman et al
J. Am. Soc. Nephrol. (2016)
Fu, Zhu, Richman et al
J. Am. Soc. Nephrol. (2016)
AI Summary: OriGene contributed to this study by providing the APOL1-G0 cDNA, which was used to generate transgenic Drosophila models for investigating the effects of APOL1 variants on nephrocyte function and cell death.
Snippet: ...analysis using anti-FLAG antibody of total protein extracts...APOL1-G0 cDNA was obtained from OriGene, which encodes the common...incubated with anti-FLAG antibody according to standard protocols...the University of Iowa for antibodies. Z.H. is supported by...
Showing 1-2 of 2 papers.
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in shipping.
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