CDC7 (NM_003503) Human Tagged ORF Clone
- TrueORF®
SKU
RG210742
CDC7 (tGFP-tagged) - Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1
$1,001.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CDC7 |
| Synonyms | CDC7L1; HsCDC7; Hsk1; huCDC7 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003503 |
| ORF Size | 1722 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003503.4 |
| RefSeq Size | 3178 bp |
| RefSeq ORF | 1725 bp |
| Locus ID | 8317 |
| UniProt ID | O00311 |
| Cytogenetics | 1p22.1 |
| Domains | pkinase, S_TKc, TyrKc |
| Protein Families | Druggable Genome, Protein Kinase, Stem cell - Pluripotency |
| Protein Pathways | Cell cycle |
| Summary | This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. provided by RefSeq, Aug 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003503" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210742 | CDC7 (Myc-DDK-tagged)-Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1 | 10 ug |
$801.00
|
|
| RC210742L1 | Lenti ORF clone of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,101.00
|
|
| RC210742L2 | Lenti ORF clone of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1, mGFP tagged | 10 ug |
$1,101.00
|
|
| RC210742L3 | Lenti ORF clone of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,101.00
|
|
| RC210742L4 | Lenti ORF clone of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1, mGFP tagged | 10 ug |
$1,101.00
|
|
| SC126574 | CDC7 (untagged)-Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1 | 10 ug |
$535.00
|
|
| SC323385 | CDC7 (untagged)-Kinase deficient mutant (K90M) of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1 | 10 ug |
$535.00
|
|
Citations
p53 controls CDC7 levels to reinforce G1 cell cycle arrest upon genotoxic stress
Tudzarova, Mulholland, Dey et al
Cell Cycle (2016)
Tudzarova, Mulholland, Dey et al
Cell Cycle (2016)
Cited by 22
AI Summary: OriGene products contributed to this study by providing the 3’UTR luciferase reporter clone of CDC7 for miRNA validation and the full-length CDC7 cDNA in the pCMV6-AC expression vector for rescue experiments, enabling the authors to investigate how p53 regulates CDC7 levels in response to genotoxic stress.
Snippet: Luciferase assay was obtained from Promega and performed according to manufacturer’s instructions in lysates from HCT116 cells co-transfected with 3’UTR luciferase reporter clone of CDC7 (OriGene) and miR-192/215 synthetic mimetics (Qiagen, Ltd.). For rescue experiments, the full 1725 bp CDC7 cDNA sequence inserted into pCMV6-AC expression vector (OriGene) was used, as described in 37. 3’ UTR luciferase reporter clone of CDC7 for miRNA validation was obtained from OriGene
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
