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CDC7 (NM_003503) Human Untagged Clone

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SKU
SC323385
CDC7 (untagged)-Kinase deficient mutant (K90M) of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1
  $535.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CDC7
Synonyms CDC7L1; HsCDC7; Hsk1; huCDC7
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_003503, the custom clone sequence may differ by one or more nucleotides


ATGGAGGCGTCTTTGGGGATTCAGATGGATGAGCCAATGGCTTTTTCTCCCCAGCGTGACCGGTTTCAGG
CTGAAGGCTCTTTAAAAAAAAACGAGCAGAATTTTAAACTTGCAGGTGTTAAAAAAGATATTGAGAAGCT
TTATGAAGCTGTACCACAGCTTAGTAATGTGTTTAAGATTGAGGACAAAATTGGAGAAGGCACTTTCAGC
TCTGTTTATTTGGCCACAGCACAGTTACAAGTAGGACCTGAAGAGAAAATTGCTCTAAAACACTTGATTC
CAACAAGTCATCCTATAAGAATTGCAGCTGAACTTCAGTGCCTAACAGTGGCTGGGGGGCAAGATAATGT
CATGGGAGTTAAATACTGCTTTAGGAAGAATGATCATGTAGTTATTGCTATGCCATATCTGGAGCATGAG
TCGTTTTTGGACATTCTGAATTCTCTTTCCTTTCAAGAAGTACGGGAATATATGCTTAATCTGTTCAAAG
CTTTGAAACGCATTCATCAGTTTGGTATTGTTCACCGTGATGTTAAGCCCAGCAATTTTTTATATAATAG
GCGCCTGAAAAAGTATGCCTTGGTAGACTTTGGTTTGGCCCAAGGAACCCATGATACGAAAATAGAGCTT
CTTAAATTTGTCCAGTCTGAAGCTCAGCAGGAAAGGTGTTCACAAAACAAATCCCACATAATCACAGGAA
ACAAGATTCCACTGAGTGGCCCAGTACCTAAGGAGCTGGATCAGCAGTCCACCACAAAAGCTTCTGTTAA
AAGACCCTACACAAATGCACAAATTCAGATTAAACAAGGAAAAGACGGAAAGGAGGGATCTGTAGGCCTT
TCTGTCCAGCGCTCTGTTTTTGGAGAAAGAAATTTCAATATACACAGCTCCATTTCACATGAGAGCCCTG
CAGTGAAACTCATGAAGCAGTCAAAGACTGTGGATGTACTGTCTAGAAAGTTAGCAACAAAAAAGAAGGC
TATTTCTACAAAAGTTATGAATAGTGCTGTGATGAGGAAAACTGCCAGTTCTTGCCCAGCTAGCCTGACC
TGTGACTGCTATGCAACAGATAAAGTTTGTAGTATTTGCCTTTCAAGGCGTCAGCAGGTTGCCCCTAGGG
CAGGTACACCAGGATTCAGAGCACCAGAGGTCTTGACAAAGTGCCCCAATCAAACTACAGCAATTGACAT
GTGGTCTGCAGGTGTCATATTTCTTTCTTTGCTTAGTGGACGATATCCATTTTATAAAGCAAGTGATGAT
TTAACTGCTTTGGCCCAAATTATGACAATTAGGGGATCCAGAGAAACTATCCAAGCTGCTAAAACTTTTG
GGAAATCAATATTATGTAGCAAAGAAGTTCCAGCACAAGACTTGAGAAAACTCTGTGAGAGACTCAGGGG
TATGGATTCTAGCACTCCCAAGTTAACAAGTGATATACAAGGGCATGCTTCTCATCAACCAGCTATTTCA
GAGAAGACTGACCATAAAGCTTCTTGCCTCGTTCAAACACCTCCAGGACAATACTCAGGGAATTCATTTA
AAAAGGGGGATAGTAATAGCTGTGAGCATTGTTTTGATGAGTATAATACCAATTTAGAAGGCTGGAATGA
GGTACCTGATGAAGCTTATGACCTGCTTGATAAACTTCTAGATCTAAATCCAGCTTCAAGAATAACAGCA
GAAGAAGCTTTGTTGCATCCATTTTTTAAAGATATGAGCTTGTGA

5' Read Nucleotide Sequence
>OriGene 5' read for mutant NM_003503 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAAT
TCGGCACGAGGGGAAGTGTTGCGCAGGCGCATCCGATCGACTCGCTGCTTTGCTCCCCCTGTGGATGTAA
CCCCTTAGCTGGCATTTTGCATCTCAATTGGCTTGTGATGGAGGCGTCTTTGGGGATTCAGATGGATGAG
CCAATGGCTTTTTCTCCCCAGCGTGACCGGTTTCAGGGCTGAAGGGCTCTTTAAAAAAAAAACGAGGCAG
GAGTTTTTAAACTTGGCAGGTGTTAAAAAAGAATATTGAAGAACCTTTTATGAAAGCCGTTCCCACAGGC
TTAGTAATGGTGGTTAAAAATGGAGGACAAAATTTGAAAAAGGCCCTTCCACCTCTGGTTTATTTGGCCC
CCCACCCCATTTCCAATTAGGGACCGGAAAAAAAAAATGGGCCCTATTGCCCTGGATCCCCACCAAGTCC
TCCCTAAAAAAATTTGCACCTGGACCTCCCGGGCCAAACGGGGGGCGGGGGGGGAAAAAAAAGGGCCTGG
GGGTTAAAAACCGGGTTTTGAAAAAAAAAACCGGGGTGAAAGCGGCAACCCCATTTTGGGACCCAAGTCC
CTTTTTTGGAATTTTGAAAATTCTTTTCCCTTTCCCAAAATTCGGGGAAAATGCCCCTAATCGTTCTAAA
CCTTTGAACCGCTTCTCACATGTTTTGGTTGTGTCTCCGGTGTTTTCCCCCCCCATTTTTTTAATAAAAC
GCCCCTGAAAATATCGCTGATGGGAACTAGTGTTGTGCCCAGAGCCCCACTAATAACAAAAGAACATCTT
ATTTTTCGCCCTAACTACGCACAAGAAGAATTGTACCCAAAACCCTAAAG
Kinase Domain Sequence
>SC323385 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSATGMGCAATGGGCGGTAGSGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCAC
GAGGGGAAGTGTTGCGCAGGCGCATCCGATCGACTCGCTGCTTTGCTCCCCCTGTGGATGTAACCCCTTA
GCTGGCATTTTGCATCTCAATTGGCTTGTGATGGAGGCGTCTTTG
Restriction Sites Please inquire |
ACCN NM_003503
Insert Size 3100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003503.2, NP_003494.1
RefSeq Size 3178 bp
RefSeq ORF 1725 bp
Locus ID 8317
UniProt ID O00311
Cytogenetics 1p22.1
Domains pkinase, S_TKc, TyrKc
Protein Families Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Protein Pathways Cell cycle
Summary This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. provided by RefSeq, Aug 2008
Transcript Variant: This variant (1) represents the most commonly occurring transcript. Variants 1, 2, and 3 all encode the same protein.
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Documents
Resources
"NM_003503" in other vectors (7)
SKU Description Size Price
RC210742 CDC7 (Myc-DDK-tagged)-Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1 10 ug
$801.00
RC210742L1 Lenti ORF clone of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1, Myc-DDK-tagged 10 ug
$1,101.00
RC210742L2 Lenti ORF clone of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1, mGFP tagged 10 ug
$1,101.00
RC210742L3 Lenti ORF clone of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1, Myc-DDK-tagged 10 ug
$1,101.00
RC210742L4 Lenti ORF clone of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1, mGFP tagged 10 ug
$1,101.00
RG210742 CDC7 (tGFP-tagged) - Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1 10 ug
$1,001.00
SC126574 CDC7 (untagged)-Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1 10 ug
$535.00

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