PREB (NM_013388) Human Tagged ORF Clone

SKU
RG208351
PREB (tGFP-tagged) - Human prolactin regulatory element binding (PREB)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$489.00 MSRP $657.00 MSRP $657.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PREB
Synonyms SEC12
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG208351 representing NM_013388
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCGGCGCCGGGCGCCAGAGCTGTACCGGGCTCCGTTCCCGTTGTACGCGCTTCAGGTCGACCCCA
GCACTGGGCTGCTCATCGCTGCGGGCGGAGGAGGCGCCGCCAAGACAGGCATAAAGAATGGCGTGCACTT
TCTGCAGCTAGAGCTGATTAATGGGCGCTTGAGTGCCTCCTTGCTGCACTCCCATGACACAGAGACACGG
GCCACCATGAACTTGGCACTGGCTGGTGACATCCTTGCTGCAGGGCAGGATGCCCACTGTCAGCTCCTGC
GCTTCCAGGCACATCAACAGCAGGGCAACAAGGCAGAGAAGGCCGGTTCCAAGGAGCAGGGGCCTCGACA
AAGGAAGGGAGCAGCCCCAGCAGAGAAGAAATGTGGAGCGGAAACCCAGCACGAGGGGCTAGAACTCAGG
GTAGAGAATTTGCAGGCGGTGCAGACAGACTTTAGCTCCGATCCACTGCAGAAAGTTGTGTGCTTCAACC
ACGATAATACCCTGCTTGCCACTGGAGGAACAGATGGCTACGTCCGTGTCTGGAAGGTGCCCAGCCTGGA
GAAGGTTCTGGAGTTCAAAGCCCACGAAGGGGAGATTGAAGACCTGGCTTTAGGGCCTGATGGCAAGTTG
GTAACCGTGGGCCGGGACCTTAAGGCCTCTGTGTGGCAGAAGGATCAGCTGGTGACACAGCTGCACTGGC
AAGAAAATGGACCCACCTTTTCCAGCACACCTTACCGCTACCAGGCCTGCAGGTTTGGGCAGGTTCCAGA
CCAGCCTGCTGGCCTGCGACTCTTCACAGTGCAAATTCCCCACAAGCGCCTGCGCCAGCCCCCTCCCTGC
TACCTCACAGCCTGGGATGGCTCCAACTTCTTGCCCCTTCGGACCAAGTCCTGTGGCCATGAAGTCGTCT
CCTGCCTCGATGTCAGTGAATCCGGCACCTTCCTAGGCCTGGGCACAGTCACTGGCTCTGTTGCCATCTA
CATAGCTTTCTCTCTCCAGTGCCTCTACTACGTGAGGGAGGCCCATGGCATTGTGGTGACGGATGTGGCC
TTTCTACCTGAGAAGGGTCGTGGTCCAGAGCTCCTTGGGTCCCATGAAACTGCCCTGTTCTCTGTGGCTG
TGGACAGTCGTTGCCAGCTGCATCTGTTGCCCTCACGGCGGAGTGTTCCTGTGTGGCTCCTGCTCCTGCT
GTGTGTCGGGCTTATTATTGTGACCATCCTGCTGCTCCAGAGTGCCTTTCCAGGTTTCCTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG208351 representing NM_013388
Red=Cloning site Green=Tags(s)

MGRRRAPELYRAPFPLYALQVDPSTGLLIAAGGGGAAKTGIKNGVHFLQLELINGRLSASLLHSHDTETR
ATMNLALAGDILAAGQDAHCQLLRFQAHQQQGNKAEKAGSKEQGPRQRKGAAPAEKKCGAETQHEGLELR
VENLQAVQTDFSSDPLQKVVCFNHDNTLLATGGTDGYVRVWKVPSLEKVLEFKAHEGEIEDLALGPDGKL
VTVGRDLKASVWQKDQLVTQLHWQENGPTFSSTPYRYQACRFGQVPDQPAGLRLFTVQIPHKRLRQPPPC
YLTAWDGSNFLPLRTKSCGHEVVSCLDVSESGTFLGLGTVTGSVAIYIAFSLQCLYYVREAHGIVVTDVA
FLPEKGRGPELLGSHETALFSVAVDSRCQLHLLPSRRSVPVWLLLLLCVGLIIVTILLLQSAFPGFL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_013388
ORF Size 1251 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013388.6
RefSeq Size 2211 bp
RefSeq ORF 1254 bp
Locus ID 10113
UniProt ID Q9HCU5
Cytogenetics 2p23.3
Domains WD40
Protein Families Transcription Factors, Transmembrane
Summary This gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. This protein may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p. This gene overlaps the abhydrolase domain containing 1 (ABHD1) gene on the opposite strand. [provided by RefSeq, Jul 2008]
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SKU Description Size Price
RC208351 PREB (Myc-DDK-tagged)-Human prolactin regulatory element binding (PREB) 10 ug
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RC208351L1 Lenti ORF clone of Human prolactin regulatory element binding (PREB), Myc-DDK-tagged 10 ug
$757.00
RC208351L2 Lenti ORF clone of Human prolactin regulatory element binding (PREB), mGFP tagged 10 ug
$757.00
RC208351L3 Lenti ORF clone of Human prolactin regulatory element binding (PREB), Myc-DDK-tagged 10 ug
$757.00
RC208351L4 Lenti ORF clone of Human prolactin regulatory element binding (PREB), mGFP tagged 10 ug
$757.00
SC319881 PREB (untagged)-Human prolactin regulatory element binding (PREB) 10 ug
$457.00

Citations

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