MCU (NM_138357) Human Tagged ORF Clone

  • TrueORF®
SKU
RG206952
MCU (tGFP-tagged) - Human coiled-coil domain containing 109A (CCDC109A)
$489.00 $657.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MCU
Synonyms C10orf42; CCDC109A; HsMCU
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG206952 representing NM_138357
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCGCCGCAGGTAGATCGCTCCTGCTGCTCCTCTCCTCTCGGGGCGGCGGCGGCGGGGGCGCCG
GCGGCTGCGGGGCGCTGACTGCCGGCTGCTTCCCTGGGCTGGGCGTCAGCCGCCACCGGCAGCAGCAGCA
CCACCGGACGGTACACCAGAGGATCGCTTCCTGGCAGAATTTGGGAGCTGTTTATTGCAGCACTGTTGTG
CCCTCTGATGATGTTACAGTGGTTTATCAAAATGGGTTACCTGTGATATCTGTGAGGCTACCATCCCGGC
GTGAACGCTGTCAGTTCACACTCAAGCCTATCTCTGACTCTGTTGGTGTATTTTTACGACAACTGCAAGA
AGAGGATCGGGGAATTGACAGAGTTGCTATCTATTCACCAGATGGTGTTCGCGTTGCTGCTTCAACAGGA
ATAGACCTCCTCCTCCTTGATGACTTTAAGCTGGTCATTAATGACTTAACATACCACGTACGACCACCAA
AAAGAGACCTCTTAAGTCATGAAAATGCAGCAACGCTGAATGATGTAAAGACATTGGTCCAGCAACTATA
CACCACACTGTGCATTGAGCAGCACCAGTTAAACAAGGAAAGGGAGCTTATTGAAAGACTAGAGGATCTC
AAAGAGCAGCTGGCTCCCCTGGAAAAGGTACGAATTGAGATTAGCAGAAAAGCTGAGAAGAGGACCACTT
TGGTGCTATGGGGTGGCCTTGCCTACATGGCCACACAGTTTGGCATTTTGGCCCGGCTTACCTGGTGGGA
ATATTCCTGGGACATCATGGAGCCAGTAACATACTTCATCACTTATGGAAGTGCCATGGCAATGTATGCA
TATTTTGTAATGACACGCCAGGAATATGTTTATCCAGAAGCCAGAGACAGACAATACTTACTATTTTTCC
ATAAAGGAGCCAAAAAGTCACGTTTTGACCTAGAGAAATACAATCAACTCAAGGATGCAATTGCTCAGGC
AGAAATGGACCTTAAGAGACTGAGAGACCCATTACAAGTACATCTGCCTCTCCGACAAATTGGTGAAAAA
GAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG206952 representing NM_138357
Red=Cloning site Green=Tags(s)

MAAAAGRSLLLLLSSRGGGGGGAGGCGALTAGCFPGLGVSRHRQQQHHRTVHQRIASWQNLGAVYCSTVV
PSDDVTVVYQNGLPVISVRLPSRRERCQFTLKPISDSVGVFLRQLQEEDRGIDRVAIYSPDGVRVAASTG
IDLLLLDDFKLVINDLTYHVRPPKRDLLSHENAATLNDVKTLVQQLYTTLCIEQHQLNKERELIERLEDL
KEQLAPLEKVRIEISRKAEKRTTLVLWGGLAYMATQFGILARLTWWEYSWDIMEPVTYFITYGSAMAMYA
YFVMTRQEYVYPEARDRQYLLFFHKGAKKSRFDLEKYNQLKDAIAQAEMDLKRLRDPLQVHLPLRQIGEK
D

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138357
ORF Size 1053 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138357.2
RefSeq Size 2978 bp
RefSeq ORF 1056 bp
Locus ID 90550
UniProt ID Q8NE86
Cytogenetics 10q22.1
Domains DUF607
Protein Families Transmembrane
Summary This gene encodes a calcium transporter that localizes to the mitochondrial inner membrane. The encoded protein interacts with mitochondrial calcium uptake 1. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2012
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Documents
Resources
"NM_138357" in other vectors (6)
SKU Description Size Price
RC206952 MCU (Myc-DDK-tagged)-Human coiled-coil domain containing 109A (CCDC109A) 10 ug
$457.00
RC206952L1 Lenti ORF clone of Human coiled-coil domain containing 109A (CCDC109A), Myc-DDK-tagged 10 ug
$757.00
RC206952L2 Lenti ORF clone of Human coiled-coil domain containing 109A (CCDC109A), mGFP tagged 10 ug
$757.00
RC206952L3 Lenti ORF clone of Human coiled-coil domain containing 109A (CCDC109A), Myc-DDK-tagged 10 ug
$757.00
RC206952L4 Lenti ORF clone of Human coiled-coil domain containing 109A (CCDC109A), mGFP tagged 10 ug
$757.00
SC100813 MCU (untagged)-Human coiled-coil domain containing 109A (CCDC109A) 10 ug
$457.00

Plasmid Map for RG206952

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Cloning scheme for RG206952

Circular map for RG206952

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