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MCU (NM_138357) Human Untagged Clone

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SKU
SC100813
MCU (untagged)-Human coiled-coil domain containing 109A (CCDC109A)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MCU
Synonyms C10orf42; CCDC109A; HsMCU
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC100813 sequence for NM_138357 edited (data generated by NextGen Sequencing)
ATGGCGGCCGCCGCAGGTAGATCGCTCCTGCTGCTCCTCTCCTCTCGGGGCGGCGGCGGC
GGGGGCGCCGGCGGCTGCGGGGCGCTGACTGCCGGCTGCTTCCCTGGGCTGGGCGTCAGC
CGCCACCGGCAGCAGCAGCACCACCGGACGGTACACCAGAGGATCGCTTCCTGGCAGAAT
TTGGGAGCTGTTTATTGCAGCACTGTTGTGCCCTCTGATGATGTTACAGTGGTTTATCAA
AATGGGTTACCTGTGATATCTGTGAGGCTACCATCCCGGCGTGAACGCTGTCAGTTCACA
CTCAAGCCTATCTCTGACTCTGTTGGTGTATTTTTACGACAACTGCAAGAAGAGGATCGG
GGAATTGACAGAGTTGCTATCTATTCACCAGATGGTGTTCGCGTTGCTGCTTCAACAGGA
ATAGACCTCCTCCTCCTTGATGACTTTAAGCTGGTCATTAATGACTTAACATACCACGTA
CGACCACCAAAAAGAGACCTCTTAAGTCATGAAAATGCAGCAACGCTGAATGATGTAAAG
ACATTGGTCCAGCAACTATACACCACACTGTGCATTGAGCAGCACCAGTTAAACAAGGAA
AGGGAGCTTATTGAAAGACTAGAGGATCTCAAAGAGCAGCTGGCTCCCCTGGAAAAGGTA
CGAATTGAGATTAGCAGAAAAGCTGAGAAGAGGACCACTTTGGTGCTATGGGGTGGCCTT
GCCTACATGGCCACACAGTTTGGCATTTTGGCCCGGCTTACCTGGTGGGAATATTCCTGG
GACATCATGGAGCCAGTAACATACTTCATCACTTATGGAAGTGCCATGGCAATGTATGCA
TATTTTGTAATGACACGCCAGGAATATGTTTATCCAGAAGCCAGAGACAGACAATACTTA
CTATTTTTCCATAAAGGAGCCAAAAAGTCACGTTTTGACCTAGAGAAATACAATCAACTC
AAGGATGCAATTGCTCAGGCAGAAATGGACCTTAAGAGACTGAGAGACCCATTACAAGTA
CATCTGCCTCTCCGACAAATTGGTGAAAAAGATTGA

Clone variation with respect to NM_138357.1
5' Read Nucleotide Sequence
>OriGene 5' read for NM_138357 unedited
CCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTC
GGCACGAGGTGAGAGATGGCGGCCGCCGCAGGTAGATCGCTCCTGCTGCTCCTCTCCTCT
CGGGGCGGCGGCGGCGGGGGCGCCGGCGGCTGCGGGGCGCTGACTGCCGGCTGCTTCCCT
GGGCTGGGCGTCAGCCGCCACCGGCAGCAGCAGCACCACCGGACGGTACACCAGAGGATC
GCTTCCTGGCAGAATTTGGGAGCTGTTTATTGCAGCACTGTTGTGCCCTCTGATGATGTT
ACAGTGGTTTATCAAAATGGGTTACCTGTGATATCTGTGAGGCTACCATCCCGGCGTGAA
CGCTGTCAGTTCACACTCAAGCCTATCTCTGACTCTGTTGGTGTATTTTTACGACAACTG
CAAGAAGAGGATCGGGGAATTGACAGAGTTGCTATCTATTCACCAGATGGTGTTCGCGTT
GCTGCTTCAACAGGAATAGACCTCCTCCTCCTTGATGACTTTAAGCTGGTCATTAATGAC
TTAACATACCACGTACGACCACCAAAAAGAGACCTCTTAAGTCATGAAAATGCAGCAACG
CTGAATGATGTAAAGACATTGGTCCAGCAACTATACACCACACTGTGCATTGAGCAGCAC
CAGTTAAACAAGGAAAGGGAGCTTATTGAANGACTAGAGGATCTCANAGAGCAGCTGGCT
CCCCTGGNANAGGGTACGAATGAGATTAGCAGAAAAGCTGAGAAGAGGACCACTTTGGTG
CTATGGGGTGGNCCTGCCTACATGGCCACACAGTTGGNCATTNNTGCCCCNGCTTACCTG
NTGGGGAANTATCCTGGGACATCATGGNAGCCAGTACCATACTTCATCACTTATGGNAAG
TGCATGGNNCATGTATGCCATATTTTGTATAACCGCCCAGNATATGTTTTNCCANACN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_138357 unedited
NCCTCCTACTATGNACCGCGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTAAAAT
TGAATAAGTATTTATTGTTCCAGAAGGTACATTGCTTTTTATACATATTTCATAAATGAT
ACAGGATTTTACACATGTTCAATAGTTTGCTATTTGTTTAAAGAACTTTTCTCCATGTCC
TCCCTCCCTTGCTTCCCACACACCCCAAAAAGATTTGCTCGTTGCACTGTGCTGTTTCTT
CTTAAAAACCCTATGCACAAACAGGATAAATGGGTTAAAAATAATTCACACAACTTAACA
AAAATAAATGAGGGAAGGAGCTGACGACTGGGGGTGGTGGAGGGAGAAGGAAGTGGGCGG
AGGGTCAGGAAGTGGAGACATGCATCCTTTCATTCCGTCATGCCAATGAAAGGGGTGTTG
GCAGGAGTAGCAGCAGCAGCAGCAATCCTGGCTGGCCTTCAACCTTTGCAAGGCAACTGG
AGTCTGGGTTTGTCATTTAAAGTTTTTCTTTTCATACACAATGATCCCCAAGGAGGAAGA
AAAAGAGAAAGAAACTCCTTGCCACAATGAGGAAGGATGAATCACGCTAAAGAAAAGGCC
CATCAGTGGTGTGGCAGCCCCCATCATTCTTTCCATCCTGCCCCTACAGCAGTTAAAAAC
AGGATATTAGACACAGATTCCTTTGACTCTTCAAGCGCAGATGTCATCTGGAAATATGGG
AGAAAAAGTTTTGACAAGTATAAGATTAATTCTCTTTCCCTCAATTTAAGATAAGAAAAA
AAAAGGTGAAAAAAAGGGGCCTTAAANGAAACCCTAAAAATGTATCCCCTATTAAGAATA
AATGGACCCCTTGAGAACCCCCACCTTGATTTACACTCGAATTTTGGGAGTGGGAAG
Restriction Sites NotI-NotI |
ACCN NM_138357
Insert Size 3000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138357.1, NP_612366.1
RefSeq Size 2978 bp
RefSeq ORF 1056 bp
Locus ID 90550
UniProt ID Q8NE86
Cytogenetics 10q22.1
Domains DUF607
Protein Families Transmembrane
Summary This gene encodes a calcium transporter that localizes to the mitochondrial inner membrane. The encoded protein interacts with mitochondrial calcium uptake 1. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2012
Transcript Variant: This variant (1) encodes the longest isoform (1).
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Documents
Resources
"NM_138357" in other vectors (6)
SKU Description Size Price
RC206952 MCU (Myc-DDK-tagged)-Human coiled-coil domain containing 109A (CCDC109A) 10 ug
$457.00
RC206952L1 Lenti ORF clone of Human coiled-coil domain containing 109A (CCDC109A), Myc-DDK-tagged 10 ug
$757.00
RC206952L2 Lenti ORF clone of Human coiled-coil domain containing 109A (CCDC109A), mGFP tagged 10 ug
$757.00
RC206952L3 Lenti ORF clone of Human coiled-coil domain containing 109A (CCDC109A), Myc-DDK-tagged 10 ug
$757.00
RC206952L4 Lenti ORF clone of Human coiled-coil domain containing 109A (CCDC109A), mGFP tagged 10 ug
$757.00
RG206952 MCU (tGFP-tagged) - Human coiled-coil domain containing 109A (CCDC109A) 10 ug
$489.00 $657.00

file-alt Citations

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