CDS2 (NM_003818) Human Tagged ORF Clone

SKU RG206807

CDS2 (tGFP-tagged) - Human CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2 (CDS2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: CDS2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206807 representing NM_003818; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACAGAGCTGAGGCAGAGGGTGGCCCATGAGCCGGTTGCGCCACCCGAGGACAAGGAGTCAGAGTCAG; AAGCAAAGGTAGATGGAGAGACTGCATCGGACAGTGAGAGCCGGGCAGAATCCGCACCCCTGCCAGTCTC; TGCAGATGATACCCCGGAGGTCCTCAATAGGGCCCTTTCCAACTTGTCTTCAAGATGGAAGAACTGGTGG; GTGAGAGGCATCCTGACTTTGGCCATGATTGCATTTTTCTTCATCATCATTTACCTGGGACCAATGGTTT; TGATGATAATCGTGATGTGCGTTCAGATTAAGTGTTTCCATGAGATAATCACTATTGGCTACAACGTCTA; CCACTCATATGATCTGCCCTGGTTCAGGACGCTCAGCTGGTACTTTCTCCTGTGTGTAAACTATTTCTTC; TATGGTGAGACAGTGACGGATTACTTCTTCACCCTGGTCCAGAGAGAAGAGCCTTTGCGGATTCTCAGTA; AATACCACCGGTTCATTTCCTTTACTCTCTATCTAATAGGATTCTGCATGTTTGTACTGAGTCTGGTCAA; GAAGCATTATCGACTGCAGTTCTACATGTTTGGCTGGACCCATGTGACATTGCTGATTGTTGTAACACAG; TCACATCTTGTTATCCACAACCTATTTGAAGGAATGATCTGGTTCATTGTCCCCATATCTTGTGTGATCT; GTAATGACATCATGGCCTATATGTTTGGCTTTTTCTTTGGTCGGACCCCACTCATCAAGCTGTCCCCGAA; GAAGACCTGGGAAGGCTTCATTGGGGGCTTCTTTGCTACTGTGGTGTTTGGCCTTCTGCTGTCCTATGTG; ATGTCCGGGTACAGATGCTTTGTCTGCCCTGTGGAGTACAACAATGACACCAACAGCTTCACTGTGGACT; GTGAGCCCTCGGACCTGTTTCGCCTGCAGGAGTACAACATTCCTGGGGTGATCCAGTCAGTCATTGGCTG; GAAAACGGTCCGGATGTACCCCTTCCAGATTCACAGCATCGCTCTCTCCACCTTTGCCTCGCTCATTGGC; CCCTTTGGAGGATTCTTCGCAAGTGGATTCAAACGAGCCTTTAAAATCAAAGACTTTGCCAATACCATTC; CTGGCCATGGAGGCATCATGGATCGCTTTGACTGCCAGTATCTGATGGCCACCTTTGTCAATGTATACAT; CGCCAGTTTTATCAGAGGCCCTAACCCAAGCAAACTGATTCAGCAGTTCCTGACTTTACGGCCAGATCAG; CAGCTCCACATCTTCAACACGCTGCGGTCTCATCTGATCGACAAAGGGATGCTGACATCCACCACAGAGG; ACGAG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206807 representing NM_003818; Red=Cloning site Green=Tags(s); ; MTELRQRVAHEPVAPPEDKESESEAKVDGETASDSESRAESAPLPVSADDTPEVLNRALSNLSSRWKNWW; VRGILTLAMIAFFFIIIYLGPMVLMIIVMCVQIKCFHEIITIGYNVYHSYDLPWFRTLSWYFLLCVNYFF; YGETVTDYFFTLVQREEPLRILSKYHRFISFTLYLIGFCMFVLSLVKKHYRLQFYMFGWTHVTLLIVVTQ; SHLVIHNLFEGMIWFIVPISCVICNDIMAYMFGFFFGRTPLIKLSPKKTWEGFIGGFFATVVFGLLLSYV; MSGYRCFVCPVEYNNDTNSFTVDCEPSDLFRLQEYNIPGVIQSVIGWKTVRMYPFQIHSIALSTFASLIG; PFGGFFASGFKRAFKIKDFANTIPGHGGIMDRFDCQYLMATFVNVYIASFIRGPNPSKLIQQFLTLRPDQ; QLHIFNTLRSHLIDKGMLTSTTEDE; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003818
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003818.4
• RefSeq Size: 2711 bp
• RefSeq ORF: 1338 bp
• Locus ID: 8760
• UniProt ID: O95674
• Cytogenetics: 20p12.3
• Domains: CTP_transf_1
• Protein Families: Transmembrane
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Summary: Breakdown products of phosphoinositides are ubiquitous second messengers that function downstream of many G protein-coupled receptors and tyrosine kinases regulating cell growth, calcium metabolism, and protein kinase C activity. This gene encodes an enzyme which regulates the amount of phosphatidylinositol available for signaling by catalyzing the conversion of phosphatidic acid to CDP-diacylglycerol. This enzyme is an integral membrane protein localized to two subcellular domains, the matrix side of the inner mitochondrial membrane where it is thought to be involved in the synthesis of phosphatidylglycerol and cardiolipin and the cytoplasmic side of the endoplasmic reticulum where it functions in phosphatidylinositol biosynthesis. Two genes encoding this enzyme have been identified in humans, one mapping to human chromosome 4q21 and a second to 20p13. [provided by RefSeq, Jul 2008]