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UBE2U (NM_152489) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG206711
UBE2U (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2U (putative) (UBE2U)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "UBE2U or NM_152489"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol UBE2U
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG206711 representing NM_152489
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACGGCAGAGCTTACCTCTTGCTGCACAGAGACTTCTGTGATCTCAAGGAGAACAATTATAAGGGTA
TCACTGCTAAGCCTGTAAGTGAAGATATGATGGAATGGGAAGTTGAAATTGAAGGTCTACAGAATTCAGT
TTGGCAGGGTTTAGTCTTCCAACTGACAATACATTTTACATCGGAGTACAACTATGCTCCTCCAGTTGTG
AAATTTATAACAATTCCGTTTCATCCAAATGTAGACCCACACACTGGTCAGCCCTGTATAGACTTTTTGG
ACAACCCTGAGAAGTGGAATACAAACTATACATTGAGCAGCATCTTACTTGCCCTACAGGTTATGCTTTC
TAATCCAGTGCTAGAGAATCCAGTGAATTTGGAAGCAGCCAGAATACTGGTTAAAGATGAATCTCTGTAC
AGAACAATTCTAAGACTTTTCAACAGGCCATTACAAATGAAAGATGACAGCCAGGAGTTACCTAAAGACC
CACGTAAATGTATCAGACCAATTAAAACAACCTCATTTAGTGATTACTACCAGACATGGTCCAGAATAGC
TACATCAAAAGCCACAGAATACTACAGAACTCCATTGCTCAAAGTTCCAAATTTCATTGGACAGTATTAC
AAATGGAAGAAAATGGATCTACAGCATCAGAAAGAATGGAATTTAAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG206711 representing NM_152489
Red=Cloning site Green=Tags(s)

MHGRAYLLLHRDFCDLKENNYKGITAKPVSEDMMEWEVEIEGLQNSVWQGLVFQLTIHFTSEYNYAPPVV
KFITIPFHPNVDPHTGQPCIDFLDNPEKWNTNYTLSSILLALQVMLSNPVLENPVNLEAARILVKDESLY
RTILRLFNRPLQMKDDSQELPKDPRKCIRPIKTTSFSDYYQTWSRIATSKATEYYRTPLLKVPNFIGQYY
KWKKMDLQHQKEWNLK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152489
ORF Size 678 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152489.3
RefSeq Size 1163 bp
RefSeq ORF 681 bp
Locus ID 148581
UniProt ID Q5VVX9
Cytogenetics 1p31.3
Protein Pathways Ubiquitin mediated proteolysis
Summary Catalyzes the covalent attachment of ubiquitin to other proteins.UniProtKB/Swiss-Prot Function
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"NM_152489" in other vectors (6)
SKU Description Size Price
RC206711 UBE2U (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2U (putative) (UBE2U) 10 ug
$289.00 $300.00
RC206711L1 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2U (putative) (UBE2U), Myc-DDK-tagged 10 ug
$600.00
RC206711L2 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2U (putative) (UBE2U), mGFP tagged 10 ug
$600.00
RC206711L3 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2U (putative) (UBE2U), Myc-DDK-tagged 10 ug
$600.00
RC206711L4 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2U (putative) (UBE2U), mGFP tagged 10 ug
$600.00
SC306416 UBE2U (untagged)-Human ubiquitin-conjugating enzyme E2U (putative) (UBE2U) 10 ug
$330.00

Plasmid Map for RG206711

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RG206711
Circular map for RG206711

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