UBE2U (NM_152489) Human Untagged Clone

SKU
SC306416
UBE2U (untagged)-Human ubiquitin-conjugating enzyme E2U (putative) (UBE2U)
  $330.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol UBE2U
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC306416 representing NM_152489.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCACGGCAGAGCTTACCTCTTGCTGCACAGAGACTTCTGTGATCTCAAGGAGAACAATTATAAGGGT
ATCACTGCTAAGCCTGTAAGTGAAGATATGATGGAATGGGAAGTTGAAATTGAAGGTCTACAGAATTCA
GTTTGGCAGGGTTTAGTCTTCCAACTGACAATACATTTTACATCGGAGTACAACTATGCTCCTCCAGTT
GTGAAATTTATAACAATTCCGTTTCATCCAAATGTAGACCCACACACTGGTCAGCCCTGTATAGACTTT
TTGGACAACCCTGAGAAGTGGAATACAAACTATACATTGAGCAGCATCTTACTTGCCCTACAGGTTATG
CTTTCTAATCCAGTGCTAGAGAATCCAGTGAATTTGGAAGCAGCCAGAATACTGGTTAAAGATGAATCT
CTGTACAGAACAATTCTAAGACTTTTCAACAGGCCATTACAAATGAAAGATGACAGCCAGGAGTTACCT
AAAGACCCACGTAAATGTATCAGACCAATTAAAACAACCTCATTTAGTGATTACTACCAGACATGGTCC
AGAATAGCTACATCAAAAGCCACAGAATACTACAGAACTCCATTGCTCAAAGTTCCAAATTTCATTGGA
CAGTATTACAAATGGAAGAAAATGGATCTACAGCATCAGAAAGAATGGAATTTAAAATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_152489
Insert Size 681 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152489.1
RefSeq Size 1163 bp
RefSeq ORF 681 bp
Locus ID 148581
UniProt ID Q5VVX9
Cytogenetics 1p31.3
Protein Pathways Ubiquitin mediated proteolysis
MW 26.7 kDa
Summary Catalyzes the covalent attachment of ubiquitin to other proteins.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_152489" in other vectors (6)
SKU Description Size Price
RC206711 UBE2U (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2U (putative) (UBE2U) 10 ug
$289.00 $300.00
RC206711L1 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2U (putative) (UBE2U), Myc-DDK-tagged 10 ug
$600.00
RC206711L2 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2U (putative) (UBE2U), mGFP tagged 10 ug
$600.00
RC206711L3 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2U (putative) (UBE2U), Myc-DDK-tagged 10 ug
$600.00
RC206711L4 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2U (putative) (UBE2U), mGFP tagged 10 ug
$600.00
RG206711 UBE2U (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2U (putative) (UBE2U) 10 ug
$489.00 $500.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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