RASA1 (NM_002890) Human Tagged ORF Clone

SKU RG205473

RASA1 (tGFP-tagged) - Human RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: RASA1
• Synonyms: CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP; p120RASGAP; PKWS; RASA; RASGAP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205473 representing NM_002890; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGATGGCGGCCGAGGCCGGCAGTGAGGAGGGCGGCCCGGTAACAGCCGGAGCTGGAGGAGGCGGCGCGG; CAGCGGGCTCCAGTGCCTATCCCGCAGTGTGTCGGGTGAAGATACCCGCGGCCCTGCCTGTGGCAGCCGC; CCCCTATCCTGGGCTGGTGGAGACCGGAGTGGCTGGAACTCTGGGTGGCGGAGCCGCTTTGGGGTCAGAG; TTCCTAGGAGCCGGGTCTGTGGCAGGGGCACTGGGGGGAGCTGGACTGACAGGGGGAGGTACTGCTGCTG; GCGTAGCTGGTGCTGCTGCTGGCGTGGCCGGTGCTGCTGTTGCTGGACCTAGTGGAGACATGGCTCTCAC; CAAACTGCCCACTTCGTTGCTTGCTGAGACTCTCGGGCCAGGCGGCGGTTTTCCCCCTCTGCCCCCTCCC; CCTTACCTGCCCCCTTTGGGGGCGGGCCTCGGGACAGTGGACGAAGGTGACTCTCTGGATGGACCAGAAT; ACGAGGAGGAAGAGGTGGCCATACCGTTGACCGCTCCTCCAACTAACCAGTGGTATCACGGAAAACTTGA; CAGAACGATAGCAGAAGAACGCCTCAGGCAGGCAGGGAAGTCTGGCAGTTATCTTATAAGAGAGAGTGAT; CGGAGGCCAGGGTCCTTTGTACTTTCATTTCTTAGCCAGATGAATGTTGTCAACCATTTTAGGATTATTG; CTATGTGTGGAGATTACTACATTGGTGGAAGACGTTTTTCTTCACTGTCAGACCTAATAGGTTATTACAG; TCATGTTTCTTGTTTGCTTAAAGGAGAAAAATTACTTTACCCAGTTGCACCACCAGAGCCAGTAGAAGAT; AGAAGGCGTGTACGAGCTATTCTACCTTACACAAAAGTACCAGACACTGATGAAATAAGTTTCTTAAAAG; GAGATATGTTCATTGTTCATAATGAATTAGAAGATGGATGGATGTGGGTTACAAATTTAAGAACAGATGA; ACAAGGCCTTATTGTTGAAGACCTAGTAGAAGAGGTGGGCCGGGAAGAAGATCCACATGAAGGAAAAATA; TGGTTCCATGGGAAGATTTCCAAACAGGAAGCTTATAATTTACTAATGACAGTTGGTCAAGTCTGCAGTT; TTCTTGTGAGGCCCTCAGATAATACTCCTGGCGATTATTCACTTTATTTCCGGACCAATGAAAATATTCA; GCGATTTAAAATATGTCCAACGCCAAACAATCAGTTTATGATGGGAGGCCGGTATTATAACAGCATTGGG; GACATCATAGATCACTATCGAAAAGAACAGATTGTTGAAGGATATTATCTTAAGGAACCTGTACCAATGC; AGGATCAAGAACAAGTACTCAATGACACAGTGGATGGCAAGGAAATCTATAATACCATCCGTCGTAAAAC; AAAGGATGCCTTTTATAAAAACATTGTTAAGAAAGGTTATCTTCTGAAAAAGGGCAAAGGAAAACGTTGG; AAAAATTTATATTTTATCTTAGAGGGTAGTGATGCCCAACTTATTTATTTTGAAAGCGAAAAACGAGCTA; CCAAACCAAAAGGATTAATAGATCTCAGTGTATGTTCTGTCTATGTCGTTCATGATAGTCTCTTTGGCAG; GCCAAACTGTTTTCAGATAGTAGTTCAGCACTTTAGTGAAGAACATTACATCTTTTACTTTGCAGGAGAA; ACTCCAGAACAAGCAGAGGATTGGATGAAAGGTCTGCAGGCATTTTGCAATTTACGGAAAAGTAGTCCAG; GGACATCCAATAAACGCCTTCGTCAGGTCAGCAGCCTTGTTTTACATATTGAAGAAGCCCATAAACTCCC; AGTAAAACATTTTACTAATCCATATTGTAACATCTACCTGAATAGTGTCCAAGTAGCAAAAACTCATGCA; AGGGAAGGGCAAAACCCAGTATGGTCAGAAGAGTTTGTCTTTGATGATCTTCCTCCTGACATCAATAGAT; TTGAAATAACTCTTAGTAATAAAACAAAGAAAAGCAAAGATCCTGATATCTTATTTATGCGCTGCCAGTT; GAGCCGATTACAGAAAGGGCATGCCACAGATGAATGGTTTCTGCTCAGCTCCCATATACCATTAAAAGGT; ATTGAACCAGGGTCCCTGCGTGTTCGAGCACGATACTCTATGGAAAAAATCATGCCAGAAGAAGAGTACA; GTGAATTTAAAGAGCTTATACTGCAAAAGGAACTTCATGTAGTCTATGCTTTATCACATGTATGTGGACA; AGACCGAACACTACTGGCCAGCATCCTACTGAGGATTTTTCTTCACGAAAAGCTTGAATCGTTGTTGTTA; TGCACACTAAATGACAGAGAAATAAGCATGGAAGATGAAGCCACTACCCTATTTCGAGCCACAACACTTG; CAAGCACCTTGATGGAGCAGTATATGAAAGCCACTGCTACACAGTTTGTTCATCATGCTTTGAAAGACTC; TATTTTAAAGATAATGGAAAGCAAGCAGTCTTGTGAGTTAAGTCCATCAAAGTTAGAAAAAAATGAAGAT; GTGAACACTAATTTAACACACCTATTGAACATACTTTCAGAGCTTGTGGAGAAAATATTCATGGCTTCAG; AAATACTTCCACCGACATTGAGATATATTTATGGGTGTTTACAGAAATCTGTTCAGCATAAGTGGCCTAC; AAATACCACCATGAGAACAAGAGTTGTTAGTGGTTTTGTTTTTCTTCGACTCATCTGTCCTGCCATCCTG; AATCCACGGATGTTCAATATCATCTCAGATTCTCCATCTCCTATTGCTGCAAGAACACTGATATTAGTGG; CTAAATCTGTGCAGAACTTAGCAAATCTTGTGGAATTTGGAGCTAAGGAGCCCTACATGGAAGGTGTCAA; TCCATTCATCAAAAGCAACAAACATCGTATGATCATGTTTTTAGATGAACTTGGGAATGTACCTGAACTT; CCGGACACTACAGAGCATTCTAGAACGGACCTGTCCCGTGATTTAGCAGCATTGCATGAGATTTGCGTGG; CTCATTCAGATGAACTTCGAACGCTCAGTAATGAGCGTGGTGCACAGCAGCACGTATTGAAAAAGCTTCT; GGCTATAACAGAACTGCTTCAACAAAAACAAAACCAGTATACAAAAACCAATGATGTCAGG; ; AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205473 representing NM_002890; Red=Cloning site Green=Tags(s); ; MMAAEAGSEEGGPVTAGAGGGGAAAGSSAYPAVCRVKIPAALPVAAAPYPGLVETGVAGTLGGGAALGSE; FLGAGSVAGALGGAGLTGGGTAAGVAGAAAGVAGAAVAGPSGDMALTKLPTSLLAETLGPGGGFPPLPPP; PYLPPLGAGLGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESD; RRPGSFVLSFLSQMNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVED; RRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKI; WFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGRYYNSIG; DIIDHYRKEQIVEGYYLKEPVPMQDQEQVLNDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRW; KNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGE; TPEQAEDWMKGLQAFCNLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHA; REGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKG; IEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLRIFLHEKLESLLL; CTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQSCELSPSKLEKNED; VNTNLTHLLNILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNTTMRTRVVSGFVFLRLICPAIL; NPRMFNIISDSPSPIAARTLILVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPEL; PDTTEHSRTDLSRDLAALHEICVAHSDELRTLSNERGAQQHVLKKLLAITELLQQKQNQYTKTNDVR; ; SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002890
• ORF Size: 3141 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002890.3
• RefSeq Size: 4307 bp
• RefSeq ORF: 3144 bp
• Locus ID: 5921
• UniProt ID: P20936
• Cytogenetics: 5q14.3
• Domains: C2, PH, RasGAP, SH2, SH3
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, MAPK signaling pathway
• Summary: The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012]