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PNPLA8 (NM_015723) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG205193
PNPLA8 (tGFP-tagged) - Human patatin-like phospholipase domain containing 8 (PNPLA8)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$789.00 $916.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PNPLA8
Synonyms IPLA2-2; IPLA2G; iPLA2gamma; MMLA; PNPLA-gamma
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG205193 representing NM_015723
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTATTAATCTGACTGTAGATATATATATTTACCTCCTTAGTAATGCAAGAAGTGTTTGTGGGAAGC
AGAGAAGCAAGCAACTGTATTTCTTGTTCTCACCTAAGCATTACTGGAGGATAAGCCACATCAGTCTACA
AAGAGGTTTTCATACAAACATAATAAGATGTAAATGGACCAAAAGTGAAGCACATTCTTGCAGTAAGCAC
TGTTACTCTCCAAGCAACCATGGTTTACATATTGGGATTTTGAAACTTAGCACTTCTGCTCCCAAGGGAC
TTACAAAAGTGAACATTTGTATGTCCCGTATTAAAAGTACTTTGAACTCTGTTTCAAAGGCTGTTTTTGG
CAATCAAAATGAAATGATTTCACGTTTAGCTCAATTTAAGCCAAGTTCCCAAATTTTAAGAAAAGTATCG
GATAGTGGCTGGTTAAAACAGAAAAACATCAAACAAGCCATCAAATCTCTGAAAAAATATAGTGACAAAT
CAGCAGAAAAGAGTCCTTTTCCAGAAGAGAAAAGTCACATTATAGACAAAGAAGAAGATATAGGTAAACG
CAGTCTTTTTCATTACACAAGTTCTATAACCACAAAATTTGGAGACTCATTCTACTTTTTATCAAATCAT
ATTAATTCATATTTCAAACGTAAGGAAAAAATGTCTCAACAAAAGGAAAATGAACATTTCCGGGACAAAT
CAGAACTTGAAGATAAAAAGGTAGAAGAGGGGAAATTAAGATCTCCAGATCCTGGCATCCTGGCTTATAA
GCCAGGCTCAGAATCTGTACATACGGTGGACAAGCCTACAAGTCCTTCTGCGATACCTGATGTTCTTCAA
GTTTCAACTAAACAAAGTATTGCTAACTTTCTTTCTCGTCCCACGGAAGGTGTACAAGCTTTAGTAGGTG
GTTATATTGGTGGACTTGTCCCCAAATTAAAGTATGATTCAAAGAGTCAGTCAGAAGAACAGGAAGAGCC
TGCTAAAACTGATCAGGCTGTCAGCAAAGACAGAAATGCAGAGGAGAAAAAGCGTTTATCTCTTCAGCGA
GAAAAGATTATCGCAAGGGTGAGTATTGATAACAGGACCCGGGCATTAGTTCAGGCATTAAGAAGAACAA
CTGACCCAAAGCTCTGCATTACTAGGGTTGAAGAACTGACTTTTCATCTTCTAGAATTTCCTGAAGGAAA
AGGAGTGGCTGTCAAGGAAAGAATTATTCCATATTTATTACGACTGAGACAAATTAAGGATGAAACTCTT
CAGGCTGCAGTTAGAGAAATTTTGGCCCTAATTGGCTATGTGGATCCAGTGAAAGGGAGAGGAATCCGAA
TTCTCTCAATTGATGGTGGAGGAACAAGGGGCGTGGTTGCTCTCCAGACCCTACGAAAATTAGTTGAACT
TACTCAGAAGCCAGTTCATCAGCTCTTTGATTACATTTGTGGTGTAAGCACAGGTGCCATATTAGCTTTC
ATGTTGGGGTTGTTTCATATGCCCTTGGATGAATGTGAGGAACTTTATCGAAAATTAGGATCAGATGTAT
TTTCACAAAATGTCATTGTTGGAACAGTAAAAATGAGTTGGAGCCATGCATTTTATGACAGTCAAACATG
GGAAAACATTCTTAAGGATAGGATGGGATCTGCACTGATGATTGAAACAGCAAGAAACCCCACATGTCCT
AAGGTAGCTGCTGTAAGTACCATAGTAAATAGAGGGATAACACCCAAAGCTTTTGTGTTCAGAAACTATG
GTCATTTTCCTGGAATCAACTCTCATTATTTGGGAGGCTGTCAGTATAAAATGTGGCAGGCCATTAGAGC
CTCATCTGCTGCTCCAGGCTACTTTGCAGAATATGCATTGGGAAATGATCTTCATCAAGATGGAGGTTTG
CTTCTGAATAACCCTTCGGCATTAGCTATGCATGAGTGTAAATGTCTTTGGCCAGATGTGCCGTTAGAGT
GCATAGTATCCCTGGGCACTGGACGTTATGAGAGTGATGTGAGAAACACGGTAACATACACAAGCTTGAA
AACTAAACTTTCTAATGTTATCAACAGTGCTACAGATACAGAAGAAGTCCATATAATGCTTGATGGCCTG
TTACCTCCTGACACCTATTTTAGATTCAATCCTGTAATGTGTGAAAACATACCTCTAGATGAAAGTCGAA
ATGAAAAGCTGGATCAGCTGCAGTTGGAAGGGTTGAAATACATAGAAAGAAATGAACAAAAAATGAAAAA
AGTTGCAAAAATATTAAGTCAAGAAAAAACAACTCTGCAGAAAATTAATGATTGGATAAAATTAAAAACT
GATATGTATGAAGGACTTCCATTCTTTTCAAAATTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG205193 representing NM_015723
Red=Cloning site Green=Tags(s)

MSINLTVDIYIYLLSNARSVCGKQRSKQLYFLFSPKHYWRISHISLQRGFHTNIIRCKWTKSEAHSCSKH
CYSPSNHGLHIGILKLSTSAPKGLTKVNICMSRIKSTLNSVSKAVFGNQNEMISRLAQFKPSSQILRKVS
DSGWLKQKNIKQAIKSLKKYSDKSAEKSPFPEEKSHIIDKEEDIGKRSLFHYTSSITTKFGDSFYFLSNH
INSYFKRKEKMSQQKENEHFRDKSELEDKKVEEGKLRSPDPGILAYKPGSESVHTVDKPTSPSAIPDVLQ
VSTKQSIANFLSRPTEGVQALVGGYIGGLVPKLKYDSKSQSEEQEEPAKTDQAVSKDRNAEEKKRLSLQR
EKIIARVSIDNRTRALVQALRRTTDPKLCITRVEELTFHLLEFPEGKGVAVKERIIPYLLRLRQIKDETL
QAAVREILALIGYVDPVKGRGIRILSIDGGGTRGVVALQTLRKLVELTQKPVHQLFDYICGVSTGAILAF
MLGLFHMPLDECEELYRKLGSDVFSQNVIVGTVKMSWSHAFYDSQTWENILKDRMGSALMIETARNPTCP
KVAAVSTIVNRGITPKAFVFRNYGHFPGINSHYLGGCQYKMWQAIRASSAAPGYFAEYALGNDLHQDGGL
LLNNPSALAMHECKCLWPDVPLECIVSLGTGRYESDVRNTVTYTSLKTKLSNVINSATDTEEVHIMLDGL
LPPDTYFRFNPVMCENIPLDESRNEKLDQLQLEGLKYIERNEQKMKKVAKILSQEKTTLQKINDWIKLKT
DMYEGLPFFSKL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015723
ORF Size 2346 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015723.5
RefSeq Size 3548 bp
RefSeq ORF 2349 bp
Locus ID 50640
UniProt ID Q9NP80
Cytogenetics 7q31.1
Summary This gene encodes a member of the patatin-like phospholipase domain containing protein family. Members of this family are phospholipases which catalyze the cleavage of fatty acids from membrane phospholipids. The product of this gene is a calcium-independent phospholipase. Mutations in this gene have been associated with mitochondrial myopathy with lactic acidosis. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, May 2015
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"NM_015723" in other vectors (6)
SKU Description Size Price
RC205193 PNPLA8 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 8 (PNPLA8) 10 ug
$589.00 $716.00
RC205193L1 Lenti ORF clone of Human patatin-like phospholipase domain containing 8 (PNPLA8), Myc-DDK-tagged 10 ug
$1,016.00
RC205193L2 Lenti ORF clone of Human patatin-like phospholipase domain containing 8 (PNPLA8), mGFP tagged 10 ug
$1,016.00
RC205193L3 Lenti ORF clone of Human patatin-like phospholipase domain containing 8 (PNPLA8), Myc-DDK-tagged 10 ug
$1,016.00
RC205193L4 Lenti ORF clone of Human patatin-like phospholipase domain containing 8 (PNPLA8), mGFP tagged 10 ug
$1,016.00
SC101159 PNPLA8 (untagged)-Human patatin-like phospholipase domain containing 8 (PNPLA8) 10 ug
$717.00

Plasmid Map for RG205193

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG205193
Circular map for RG205193

file-alt Citations

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