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PNPLA8 (NM_015723) Human Untagged Clone

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SKU
SC101159
PNPLA8 (untagged)-Human patatin-like phospholipase domain containing 8 (PNPLA8)
  $717.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PNPLA8
Synonyms IPLA2-2; IPLA2G; iPLA2gamma; MMLA; PNPLA-gamma
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_015723, the custom clone sequence may differ by one or more nucleotides


ATGTCTATTAATCTGACTGTAGATATATATATTTACCTCCTTAGTAATGCAAGAAGTGTTTGTGGGAAGC
AGAGAAGCAAGCAACTGTATTTCTTGTTCTCACCTAAGCATTACTGGAGGATAAGCCACATCAGTCTACA
AAGAGGTTTTCATACAAACATAATAAGATGTAAATGGACCAAAAGTGAAGCACATTCTTGCAGTAAGCAC
TGTTACTCTCCAAGCAACCATGGTTTACATATTGGGATTTTGAAACTTAGCACTTCTGCTCCCAAGGGAC
TTACAAAAGTGAACATTTGTATGTCCCGTATTAAAAGTACTTTGAACTCTGTTTCAAAGGCTGTTTTTGG
CAATCAAAATGAAATGATTTCACGTTTAGCTCAATTTAAGCCAAGTTCCCAAATTTTAAGAAAAGTATCG
GATAGTGGCTGGTTAAAACAGAAAAACATCAAACAAGCCATCAAATCTCTGAAAAAATATAGTGACAAAT
CAGCAGAAAAGAGTCCTTTTCCAGAAGAGAAAAGTCACATTATAGACAAAGAAGAAGATATAGGTAAACG
CAGTCTTTTTCATTACACAAGTTCTATAACCACAAAATTTGGAGACTCATTCTACTTTTTATCAAATCAT
ATTAATTCATATTTCAAACGTAAGGAAAAAATGTCTCAACAAAAGGAAAATGAACATTTCCGGGACAAAT
CAGAACTTGAAGATAAAAAGGTAGAAGAGGGGAAATTAAGATCTCCAGATCCTGGCATCCTGGCTTATAA
GCCAGGCTCAGAATCTGTACATACGGTGGACAAGCCTACAAGTCCTTCTGCGATACCTGATGTTCTTCAA
GTTTCAACTAAACAAAGTATTGCTAACTTTCTTTCTCGTCCCACGGAAGGTGTACAAGCTTTAGTAGGTG
GTTATATTGGTGGACTTGTCCCCAAATTAAAGTATGATTCAAAGAGTCAGTCAGAAGAACAGGAAGAGCC
TGCTAAAACTGATCAGGCTGTCAGCAAAGACAGAAATGCAGAGGAGAAAAAGCGTTTATCTCTTCAGCGA
GAAAAGATTATCGCAAGGGTGAGTATTGATAACAGGACCCGGGCATTAGTTCAGGCATTAAGAAGAACAA
CTGACCCAAAGCTCTGCATTACTAGGGTTGAAGAACTGACTTTTCATCTTCTAGAATTTCCTGAAGGAAA
AGGAGTGGCTGTCAAGGAAAGAATTATTCCATATTTATTACGACTGAGACAAATTAAGGATGAAACTCTT
CAGGCTGCAGTTAGAGAAATTTTGGCCCTAATTGGCTATGTGGATCCAGTGAAAGGGAGAGGAATCCGAA
TTCTCTCAATTGATGGTGGAGGAACAAGGGGCGTGGTTGCTCTCCAGACCCTACGAAAATTAGTTGAACT
TACTCAGAAGCCAGTTCATCAGCTCTTTGATTACATTTGTGGTGTAAGCACAGGTGCCATATTAGCTTTC
ATGTTGGGGTTGTTTCATATGCCCTTGGATGAATGTGAGGAACTTTATCGAAAATTAGGATCAGATGTAT
TTTCACAAAATGTCATTGTTGGAACAGTAAAAATGAGTTGGAGCCATGCATTTTATGACAGTCAAACATG
GGAAAACATTCTTAAGGATAGGATGGGATCTGCACTGATGATTGAAACAGCAAGAAACCCCACATGTCCT
AAGGTAGCTGCTGTAAGTACCATAGTAAATAGAGGGATAACACCCAAAGCTTTTGTGTTCAGAAACTATG
GTCATTTTCCTGGAATCAACTCTCATTATTTGGGAGGCTGTCAGTATAAAATGTGGCAGGCCATTAGAGC
CTCATCTGCTGCTCCAGGCTACTTTGCAGAATATGCATTGGGAAATGATCTTCATCAAGATGGAGGTTTG
CTTCTGAATAACCCTTCGGCATTAGCTATGCATGAGTGTAAATGTCTTTGGCCAGATGTGCCGTTAGAGT
GCATAGTATCCCTGGGCACTGGACGTTATGAGAGTGATGTGAGAAACACGGTAACATACACAAGCTTGAA
AACTAAACTTTCTAATGTTATCAACAGTGCTACAGATACAGAAGAAGTCCATATAATGCTTGATGGCCTG
TTACCTCCTGACACCTATTTTAGATTCAATCCTGTAATGTGTGAAAACATACCTCTAGATGAAAGTCGAA
ATGAAAAGCTGGATCAGCTGCAGTTGGAAGGGTTGAAATACATAGAAAGAAATGAACAAAAAATGAAAAA
AGTTGCAAAAATATTAAGTCAAGAAAAAACAACTCTGCAGAAAATTAATGATTGGATAAAATTAAAAACT
GATATGTATGAAGGACTTCCATTCTTTTCAAAATTGTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_015723 unedited
GTGCACATATTTGTATACGAACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGTTTGT
GTTGGAAGCTCAGCTGATGCAGGCCGGTTGGAGTGGACGTCATTGCCGGGAACGAGCGAG
TCGCCGCTGCAGCCCTAGTGACTGCGGCCTGCATCCCGTAATGCAAGAAGTGTTTGTGGG
AAGCAGAGAAGCAAGCAACTGTATTTCTTGTTCTCACCTAAGCATTACTGGAGGATAAGC
CACATCAGTCTACAAAGAGGTTTTCATACAAACATAATAAGATGTAAATGGACCAAAAGT
GAAGCACATTCTTGCAGTAAGCACTGTTACTCTCCAAGCAACCATGGTTTACATATTGGG
ATTTTGAAACTTAGCACTTCTGCTCCCAAGGGACTTACAAAAGTGAACATTTGTATGTCC
CGTATTAAAAGTACTTTGAACTCTGTTTCAAAGGCTGTTTTTGGCAATCAAAATGAAATG
ATTTCACGTTTAGCTCAATTTAAGCCAAGTTCCCAAATTTTAAGAAAAGTATCGGATAGT
GGCTGGTTAAAACAGAAAAACATCAAACAAGCCATCAAATCTCTGAAAAAATATAGTGAC
AAATCAGCAGAAAAGAGTCCTTTTCCAGAAGAGAAAAGTCACATTATAGACAAAGAAGAA
GATATAGGTAAACGCAGTCTTTTTCATTACACAAGTTCTATAACCACAAAATTTGGAGAC
TCATTCTACTTTTTATCANATCATATTAATTCATATNTCAAACGTAAGGAAAAAATGTCT
CAACAAAGGGANATGAACATTTCCGGGACANATCAGAACTTTGAGATAAAGGTAGAAGAG
GGGAAATAAGATCTNCAGACCTGGCATCCCTGCTATAGCCAGCTCAAATCTGTCATACGG
TGGACAGCCTACAGTCCT
3' Read Nucleotide Sequence
>OriGene 3' genomic read for NM_015723 unedited
TAACTTATGGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTGAGATGG
AGTCTCGCTGTGTCGCCTAGGCTGGAGTGCACTGGCGCTATCTGGCTCACTGCAACCTCC
GTCTCTCAGGTTCAAGCCATTCCCCTGCCTTAGCCTCCCGAGTAGCCGGGACCACAGGTG
TGCACCACCATGCCGGGCTAATGCTTGCACCCTTATTAGAGACGAGGTTTCACCGTGCTG
GCCAGGCTGGTCCCGGACTCCCGACGTCAGGTGATCTGCCCGCCTCGGCCTCCCAAAGTG
CTGGGATTACAGGCGTGAGCCACCGCGCCAGGCAACTAACCTTTTATTTTTAAACATACT
GCTGACAGGTGTATTCAAAGGCTAAAGGGGAGCTACATTATGAACAAAAATTAATTTTAA
AATGACATTAGCCTTTTAACAACAGATTATTTAAATAGTTTATTTTTGTTAATGATAGGA
ATATCTCCTCAGTAAGTTCAAACCATTTTATAACAGGGAAGAATAAGCTAGTGTTATATC
TGGAAAAGTTAATATAGCATTATCTTGAAATTTCAGGGGTAAAAAGTGGATGACTGGAAC
TTCACCTTTTTTAACCAAACAATGTTAAAATAAACATATCTGAATAGAAAACTGTGTCTG
GTTCTTTTTTATGTCAAATGTGAAATACTTTAATAGAGAAAGATCCATTTCTCTGCATCT
ATTTAGATGATATTACAATTAACAGCAGTGTGGATTGGAGAACATAGCTGTGAGAACATA
GGTAATGTAAATTCAAGAACAAATACCTTCTTCATATGACATATAATCTATGATCACATA
GAAATCACATGGATCTTGAAGACTAAAGTGAAANTCCCAGGACAATTTATCTTTCGGTTT
CATGCTCGGACATATCTTATATATTTNCAACCTTGACAATTAACACCATGTTTCTAAAC
Restriction Sites NotI-NotI |
ACCN NM_015723
Insert Size 3510 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015723.2, NP_056538.1
RefSeq Size 3548 bp
RefSeq ORF 2349 bp
Locus ID 50640
UniProt ID Q9NP80
Cytogenetics 7q31.1
Summary This gene encodes a member of the patatin-like phospholipase domain containing protein family. Members of this family are phospholipases which catalyze the cleavage of fatty acids from membrane phospholipids. The product of this gene is a calcium-independent phospholipase. Mutations in this gene have been associated with mitochondrial myopathy with lactic acidosis. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, May 2015
Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_015723" in other vectors (6)
SKU Description Size Price
RC205193 PNPLA8 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 8 (PNPLA8) 10 ug
$589.00 $716.00
RC205193L1 Lenti ORF clone of Human patatin-like phospholipase domain containing 8 (PNPLA8), Myc-DDK-tagged 10 ug
$1,016.00
RC205193L2 Lenti ORF clone of Human patatin-like phospholipase domain containing 8 (PNPLA8), mGFP tagged 10 ug
$1,016.00
RC205193L3 Lenti ORF clone of Human patatin-like phospholipase domain containing 8 (PNPLA8), Myc-DDK-tagged 10 ug
$1,016.00
RC205193L4 Lenti ORF clone of Human patatin-like phospholipase domain containing 8 (PNPLA8), mGFP tagged 10 ug
$1,016.00
RG205193 PNPLA8 (tGFP-tagged) - Human patatin-like phospholipase domain containing 8 (PNPLA8) 10 ug
$789.00 $916.00

file-alt Citations

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