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MCEE (NM_032601) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG205018
MCEE (tGFP-tagged) - Human methylmalonyl CoA epimerase (MCEE)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "MCEE or NM_032601"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MCEE
Synonyms GLOD2; MCE
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG205018 representing NM_032601
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCGGGTGCTGAAGGCTGCAGCCGCGAATGCCGTAGGGCTTTTTTCCAGACTTCAAGCTCCCATTC
CAACAGTAAGAGCTTCTTCCACATCACAGCCCTTGGATCAAGTGACAGGTTCTGTGTGGAACCTGGGTCG
ACTCAACCATGTAGCCATAGCAGTGCCAGATTTGGAAAAGGCTGCAGCATTTTATAAGAATATTCTGGGG
GCCCAGGTAAGTGAAGCGGTCCCTCTTCCTGAACATGGAGTATCTGTTGTTTTTGTCAACCTGGGAAATA
CCAAGATGGAACTGCTTCATCCATTGGGACTTGACAGTCCAATTGCAGGTTTTCTGCAGAAAAACAAGGC
TGGAGGAATGCATCACATCTGCATCGAGGTGGATAATATTAATGCAGCTGTGATGGATTTGAAAAAAAAG
AAGATCCGCAGTCTAAGTGAAGAGGTCAAAATAGGAGCACATGGAAAACCAGTGATTTTTCTCCATCCTA
AAGACTGTGGTGGAGTCCTTGTGGAACTGGAGCAAGCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG205018 representing NM_032601
Red=Cloning site Green=Tags(s)

MARVLKAAAANAVGLFSRLQAPIPTVRASSTSQPLDQVTGSVWNLGRLNHVAIAVPDLEKAAAFYKNILG
AQVSEAVPLPEHGVSVVFVNLGNTKMELLHPLGLDSPIAGFLQKNKAGGMHHICIEVDNINAAVMDLKKK
KIRSLSEEVKIGAHGKPVIFLHPKDCGGVLVELEQA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032601
ORF Size 528 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032601.2, NP_115990.2
RefSeq Size 850 bp
RefSeq ORF 531 bp
Locus ID 84693
UniProt ID Q96PE7
Cytogenetics 2p13.3
Protein Pathways leucine and isoleucine degradation, Metabolic pathways, Propanoate metabolism, Valine
Summary The product of this gene catalyzes the interconversion of D- and L-methylmalonyl-CoA during the degradation of branched chain amino acids. odd chain-length fatty acids, and other metabolites. Mutations in this gene result in methylmalonyl-CoA epimerase deficiency, which is presented as mild to moderate methylmalonic aciduria. provided by RefSeq, Jul 2008
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"NM_032601" in other vectors (6)
SKU Description Size Price
RC205018 MCEE (Myc-DDK-tagged)-Human methylmalonyl CoA epimerase (MCEE) 10 ug
$300.00
RC205018L1 Lenti ORF clone of Human methylmalonyl CoA epimerase (MCEE), Myc-DDK-tagged 10 ug
$600.00
RC205018L2 Lenti ORF clone of Human methylmalonyl CoA epimerase (MCEE), mGFP tagged 10 ug
$600.00
RC205018L3 Lenti ORF clone of Human methylmalonyl CoA epimerase (MCEE), Myc-DDK-tagged 10 ug
$600.00
RC205018L4 Lenti ORF clone of Human methylmalonyl CoA epimerase (MCEE), mGFP tagged 10 ug
$600.00
SC125797 MCEE (untagged)-Human methylmalonyl CoA epimerase (MCEE) 10 ug
$300.00

Plasmid Map for RG205018

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RG205018
Circular map for RG205018

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