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MCEE (NM_032601) Human Untagged Clone

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SKU
SC125797
MCEE (untagged)-Human methylmalonyl CoA epimerase (MCEE)
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MCEE
Synonyms GLOD2; MCE
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_032601 edited
GCTTTCCAAAATGGCGCGGGTGCTGAAGGCTGCAGCCGCGAATGCCGTAGGGCTTTTTTC
CAGACTTCAAGCTCCCATTCCAACAGTAAGAGCTTCTTCCACATCACAGCCCTTGGATCA
AGTGACAGGTTCTGTGTGGAACCTGGGTCGACTCAACCATGTAGCCATAGCAGTGCCAGA
TTTGGAAAAGGCTGCAGCATTTTATAAGAATATTCTGGGGGCCCAGGTAAGTGAAGCGGT
CCCTCTTCCTGAACATGGAGTATCTGTTGTTTTTGTCAACCTGGGAAATACCAAGATGGA
ACTGCTTCATCCATTGGGACTTGACAGTCCAATTGCAGGTTTTCTGCAGAAAAACAAGGC
TGGAGGAATGCATCACATCTGCATCGAGGTGGATAATATTAATGCAGCTGTGATGGATTT
GAAAAAAAAGAAGATCCGCAGTCTAAGTGAAGAGGTCAAAATAGGAGCACATGGAAAACC
AGTGATTTTTCTCCATCCTAAAGACTGTGGTGGAGTCCTTGTGGAACTGGAGCAAGCTTG
ATTTATATTTGCAAGCAACTAAATTAATTGACCTGAAAAAGCCTATCAAATACTATCAAA
ATGTACTATGACATTGAGTCCTTCACTGCTTCCATCATGTAAAAGTTCACAGTTAAAGAC
TGAATTACAGAAAGATTAAAATATATACATATATAAATACATAAATATGTATATTATTTA
GATTAACAAACATATTTGTTAATTTGAATTTGAAGAAAATCTTGATTACTAATTACTTAG
GGAACATTATTAAAATCATATAGAAATAAATTATTCCTCTTCAAAAAAAAAAAAAAAAAA
AAAAAAAAAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_032601 unedited
TCACGAATTTGTAATACGCACTCACTATAGGCGGCGCGCGAATTCGCCATTACGGCCGGG
GGCTTTCCAAAATGGCGCGGGTGCTGAAGGCTGCAGCCGCGAATGCCGTAGGGCTTTTTT
CCAGACTTCAAGCTCCCATTCCAACAGTAAGAGCTTCTTCCACATCACAGCCCTTGGATC
AAGTGACAGGTTCTGTGTGGAACCTGGGTCGACTCAACCATGTAGCCATAGCAGTGCCAG
ATTTGGAAAAGGCTGCAGCATTTTATAAGAATATTCTGGGGGCCCAGGTAAGTGAAGCGG
TCCCTCTTCCTGAACATGGAGTATCTGTTGTTTTTGTCAACCTGGGAAATACCAAGATGG
AACTGCTTCATCCATTGGGACTTGACAGTCCAATTGCAGGTTTTCTGCAGAAAAACAAGG
CTGGAGGAATGCATCACATCTGCATCGAGGTGGATAATATTAATGCAGCTGTGATGGATT
TGAAAAAAAAGAAGATCCGCAGTCTAAGTGAAGAGGTCAAAATAGGAGCACATGGAAAAC
CAGTGATTTTTCTCCATCCTAAAGACTGTGGTGGAGTCCTTGTGGAACTGGAGCAAGCTT
GTTTATATTTGCAAGCACTAAATTAATTGACCTGAAAAAGGCTATCAAATACTATCAAAA
TGTCTATGACATTGAGTCCTTCACTGGGTCCATCATGGTAAAGTGCACAGTTTAAGACTG
AAGTACCGGAAAGATTAAAATTTTTACCTAATTTAAATCCCTAAATTTGGGTTTTTATTT
AAAATAACAAACCTATTTGGTAAATTTGGAGTTGGAAAAAAATCTTGGTGACCAATTGAC
TTAGGGGACACTTGTTAAATCCTGTTGAGAAAAAATTAGTCCCCTCTCCGAGAAAAAGGG
GGGGGGGANNAGACATGTGTGGGCGCCTTGGCCCTCGATTCTTATTGGGGCCGCGGGATA
AAGGTGTGCGAGAAAAACCCGG
Restriction Sites Please inquire |
ACCN NM_032601
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032601.2, NP_115990.2
RefSeq Size 850 bp
RefSeq ORF 531 bp
Locus ID 84693
UniProt ID Q96PE7
Cytogenetics 2p13.3
Protein Pathways leucine and isoleucine degradation, Metabolic pathways, Propanoate metabolism, Valine
Summary The product of this gene catalyzes the interconversion of D- and L-methylmalonyl-CoA during the degradation of branched chain amino acids. odd chain-length fatty acids, and other metabolites. Mutations in this gene result in methylmalonyl-CoA epimerase deficiency, which is presented as mild to moderate methylmalonic aciduria. provided by RefSeq, Jul 2008
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"NM_032601" in other vectors (6)
SKU Description Size Price
RC205018 MCEE (Myc-DDK-tagged)-Human methylmalonyl CoA epimerase (MCEE) 10 ug
$300.00
RC205018L1 Lenti ORF clone of Human methylmalonyl CoA epimerase (MCEE), Myc-DDK-tagged 10 ug
$600.00
RC205018L2 Lenti ORF clone of Human methylmalonyl CoA epimerase (MCEE), mGFP tagged 10 ug
$600.00
RC205018L3 Lenti ORF clone of Human methylmalonyl CoA epimerase (MCEE), Myc-DDK-tagged 10 ug
$600.00
RC205018L4 Lenti ORF clone of Human methylmalonyl CoA epimerase (MCEE), mGFP tagged 10 ug
$600.00
RG205018 MCEE (tGFP-tagged) - Human methylmalonyl CoA epimerase (MCEE) 10 ug
$489.00 $500.00

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