WDR77 (NM_024102) Human Tagged ORF Clone

  • TrueORF®
SKU
RG204532
WDR77 (tGFP-tagged) - Human WD repeat domain 77 (WDR77)
  $657.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol WDR77
Synonyms HKMT1069; MEP-50; MEP50; Nbla10071; p44; p44/Mep50
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG204532 representing NM_024102
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGAAGGAAACCCCACCCCCCCTAGTGCCCCCGGCGGCCCGGGAGTGGAATCTTCCCCCAAATGCGC
CCGCCTGCATGGAACGGCAGTTGGAGGCTGCGCGGTACCGGTCCGATGGGGCGCTTCTCCTCGGGGCCTC
CAGCCTGAGTGGGCGCTGCTGGGCCGGCTCCCTCTGGCTTTTTAAGGACCCCTGTGCCGCCCCCAACGAA
GGCTTCTGCTCCGCCGGAGTCCAAACGGAGGCTGGAGTGGCTGACCTCACTTGGGTTGGGGAGAGAGGTA
TTCTAGTGGCCTCCGATTCAGGTGCTGTTGAATTGTGGGAACTAGATGAGAATGAGACACTTATTGTCAG
CAAGTTCTGCAAGTATGAGCATGATGACATTGTGTCTACAGTCAGTGTCTTGAGCTCTGGCACACAAGCT
GTCAGTGGTAGCAAAGACATCTGCATCAAGGTTTGGGACCTTGCTCAGCAGGTGGTACTGAGTTCATACC
GAGCTCATGCTGCTCAGGTCACTTGTGTTGCTGCCTCTCCTCACAAGGACTCTGTGTTTCTTTCATGCAG
CGAGGACAATAGAATTTTACTCTGGGATACCCGCTGTCCCAAGCCAGCATCACAGATTGGCTGCAGTGCG
CCTGGCTACCTTCCTACCTCGCTGGCTTGGCATCCTCAGCAAAGTGAAGTCTTTGTCTTTGGTGATGAGA
ATGGGACAGTCTCCCTTGTGGACACCAAGAGTACAAGCTGTGTCCTGAGCTCAGCTGTACACTCCCAGTG
TGTCACTGGGCTGGTGTTCTCCCCACACAGTGTTCCCTTCCTGGCCTCTCTCAGTGAAGACTGCTCACTT
GCTGTGCTGGACTCAAGCCTTTCTGAGTTGTTTAGAAGCCAAGCCCACAGAGACTTTGTGAGAGATGCGA
CTTGGTCCCCGCTCAATCACTCCCTGCTTACCACAGTGGGCTGGGACCATCAGGTCGTCCACCACGTTGT
GCCCACAGAACCTCTCCCAGCCCCTGGACCTGCAAGTGTTACTGAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG204532 representing NM_024102
Red=Cloning site Green=Tags(s)

MRKETPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGALLLGASSLSGRCWAGSLWLFKDPCAAPNE
GFCSAGVQTEAGVADLTWVGERGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVSVLSSGTQA
VSGSKDICIKVWDLAQQVVLSSYRAHAAQVTCVAASPHKDSVFLSCSEDNRILLWDTRCPKPASQIGCSA
PGYLPTSLAWHPQQSEVFVFGDENGTVSLVDTKSTSCVLSSAVHSQCVTGLVFSPHSVPFLASLSEDCSL
AVLDSSLSELFRSQAHRDFVRDATWSPLNHSLLTTVGWDHQVVHHVVPTEPLPAPGPASVTE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024102
ORF Size 1026 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024102.4
RefSeq Size 2428 bp
RefSeq ORF 1029 bp
Locus ID 79084
UniProt ID Q9BQA1
Cytogenetics 1p13.2
Domains WD40
Protein Families Stem cell - Pluripotency
Summary The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Nov 2015
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Documents
Resources
"NM_024102" in other vectors (6)
SKU Description Size Price
RC204532 WDR77 (Myc-DDK-tagged)-Human WD repeat domain 77 (WDR77) 10 ug
$457.00
RC204532L1 Lenti ORF clone of Human WD repeat domain 77 (WDR77), Myc-DDK-tagged 10 ug
$757.00
RC204532L2 Lenti ORF clone of Human WD repeat domain 77 (WDR77), mGFP tagged 10 ug
$757.00
RC204532L3 Lenti ORF clone of Human WD repeat domain 77 (WDR77), Myc-DDK-tagged 10 ug
$757.00
RC204532L4 Lenti ORF clone of Human WD repeat domain 77 (WDR77), mGFP tagged 10 ug
$757.00
SC112245 WDR77 (untagged)-Human WD repeat domain 77 (WDR77) 10 ug
$457.00

Plasmid Map for RG204532

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Cloning scheme for RG204532

Circular map for RG204532

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