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WDR77 (NM_024102) Human Untagged Clone

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SKU
SC112245
WDR77 (untagged)-Human WD repeat domain 77 (WDR77)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol WDR77
Synonyms HKMT1069; MEP-50; MEP50; Nbla10071; p44; p44/Mep50
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_024102 edited
GAATTCGGCACGAGGCTGCCGGAGCTGAGTCTCCGGCCGGCGTCCAGTTTGAGTCTAGGT
TGGAGTTGGAACCGTGGAGATGCGGAAGGAAACCCCACCCCCCCTAGTGCCCCCGGCGGC
CCGGGAGTGGAATCTTCCCCCAAATGCGCCCGCCTGCATGGAACGGCAGTTGGAGGCTGC
GCGGTACCGGTCCGATGGGGCGCTTCTCCTCGGGGCCTCCAGCCTGAGTGGGCGCTGCTG
GGCCGGCTCCCTCTGGCTTTTTAAGGACCCCTGTGCCGCCCCCAACGAAGGCTTCTGCTC
CGCCGGAGTCCAAACGGAGGCTGGAGTGGCTGACCTCACTTGGGTTGGGGAGAGAGGTAT
TCTAGTGGCCTCCGATTCAGGTGCTGTTGAATTGTGGGAACTAGATGAGAATGAGACACT
TATTGTCAGCAAGTTCTGCAAGTATGAGCATGATGACATTGTGTCTACAGTCAGTGTCTT
GAGCTCTGGCACACAAGCTGTCAGTGGTAGCAAAGACATCTGCATCAAGGTTTGGGACCT
TGCTCAGCAGGTGGTACTGAGTTCATACCGAGCTCATGCTGCTCAGGTCACTTGTGTTGC
TGCCTCTCCTCACAAGGACTCTGTGTTTCTTTCATGCAGCGAGGACAATAGAATTTTACT
CTGGGATACCCGCTGTCCCAAGCCAGCATCACAGATTGGCTGCAGTGCGCCTGGCTACCT
TCCTACCTCGCTGGCTTGGCATCCTCAGCAAAGTGAAGTCTTTGTCTTTGGTGATGAGAA
TGGGACAGTCTCCCTTGTGGACACCAAGAGTACAAGCTGTGTCCTGAGCTCAGCTGTACA
CTCCCAGTGTGTCACTGGGCTGGTGTTCTCCCCACACAGTGTTCCCTTCCTGGCCTCTCT
CAGTGAAGACTGCTCACTTGCTGTGCTGGACTCAAGCCTTTCTGAGTTGTTTAGAAGCCA
AGCCCACAGAGACTTTGTGAGAGATGCGACTTGGTCCCCGCTCAATCACTCCCTGCTTAC
CACAGTGGGCTGGGACCATCAGGTCGTCCACCACGTTGTGCCCACAGAACCTCTCCCAGC
CCCTGGACCTGCAAGTGTTACTGAGTAGATTGGATTTAAGACAAAAAGCAAGTCCCCCAT
GAGTGTCCACTTCTTTGCCCTGCCCTCTCAGCTTGTGAGACAACACAGGAGCCTTCTATA
GTATGTTGATATGCTAGATCTGTGCCGTTAATAGGCATCGTCTCTCAGCCTGAGGGAGGC
TGGATTCTGGGTTCCTGTAGTCACAGGGAGGAAAAGCTTTCTTAAAAATGGACATGTATG
TGCGTGTGAGTGTGTGTGTAGATTTATAGTTTTTGGTAGTGGCAGGAATAAAAAAAATCC
ATCCTACATCTTCCCTAAGCACTGCCTCTCTCTCACCCCCCAAAACAAGTTGACGAAAGG
GTTTTATGTAGCTGTCTATGAGGAATTGGCCGTGTCTGGGTGGGTTATGGGATGTGGGCA
TCCCTGGGTTCTTGGAAGCAGCTCTTATGCTACTCATAGAGATGGGATTGACTTTATTTT
TTTATAGTGCTTAATTCACCATTATGAGAAATGCTTCCAGTCACAAAAATGCAGCCCAGC
TCACTCTGAGGAAGAAGCAGGACTTGGTACGGTTTTACACAACTCCTTACCATTAAACTG
AATCAGAAATCCATTTTCTGGCTGAATAAAAAGTTTGGCTTGCCTGTGTAATGCCCACTC
CCTTCCCCCTGGCTCCCTAGTGATGGGACATATATGAGAGAGAAGTGTTTTTCTATCATA
GACACCATAGGGGAAAGTTTGGGGATGAAGGAGAGCTTAAAGGTGTTTCAATTAAGTTAG
AAAACTGACACAGGCTGTTGAGAATTCTTTGCCACTTTTCCCACCCCAAAACAGCATGGG
GCCTGACATCTTCTGCCCTGGTCCCCTTTCTCTTGATGTGGAAAGTCTGAATGCAGTATT
TATAGACTTCTAAGGTTTTAAAATCCAGTATCAAGAAGAAAATCAGAAATACTGGTTGGT
GAAATAAAGAGTTTAGGCATTGTTAAAAAAAAAAAAAAAAAAAAACTCGAC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_024102 unedited
TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCCGGAGCTGAGTCTCCGG
CCGGCGTCCAGTTTGAGTCTAGGTTGGAGTTGGAACCGTGGAGATGCGGAAGGAAACCCC
ACCCCCCCTAGTGCCCCCGGCGGCCCGGGAGTGGAATCTTCCCCCAAATGCGCCCGCCTG
CATGGAACGGCAGTTGGAGGCTGCGCGGTACCGGTCCGATGGGGCGCTTCTCCTCGGGGC
CTCCAGCCTGAGTGGGCGCTGCTGGGCCGGCTCCCTCTGGCTTTTTAAGGACCCCTGTGC
CGCCCCCAACGAAGGCTTCTGCTCCGCCGGAGTCCAAACGGAGGCTGGAGTGGCTGACCT
CACTTGGGTTGGGGAGAGAGGTATTCTAGTGGCCTCCGATTCAGGTGCTGTTGAATTGTG
GGAACTAGATGAGAATGAGACACTTATTGTCAGCAAGTTCTGCAAGTATGAGCATGATGA
CATTGTGTCTACAGTCAGTGTCTTGAGCTCTGGCACACAAGCTGTCAGTGGTAGCAAAGA
CATCTGCATCAAGGTTTGGGACCTTGCTCAGCAGGTGGTACTGAGTTCATACCGAGCTCA
TGCTGCTCANGTCACTTGTGTTGCTGCCTCTCCTCACAAGACTCTGTGTTTCTTTCATGC
AGCGAGGACAATAGAATTNTACTCTGGGATACCCGCTGTCCCNAGCCAGCATCACAGATT
GGCTGCAGTGCGCCTGGCTACCTTNCTACCTCGCTGGCTTGGCATCCTCACANAGTGAAG
TCTTTGCTTTGGTGATGAGATGGGGACAGTCTCCCTTGGGGCACCCAGATAAAACCTGTG
TCCTGAGCTCAACTGACACTCCCATGTGTCCTGGGGGTGGTGGTCCTCCCCACAAGGTTC
CTTCCTGGCCTTTTAAAGAAACAGCCCATTCTTGGCTGACTAGACTTT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_024102 unedited
NNTTTTTGACTATGNACCCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTTTT
TTAACAATGCCTAAACTCTTTATTTCACCAACCAGTATTTCTGATTTTCTTCTTGATACT
GGATTTTAAAACCTTAGAAGTCTATAAATACTGCATTCAGACTTTCCACATCAAGAGAAA
GGGGACCAGGGCAGAAGATGTCAGGCCCCATGCTGTTTTGGGGTGGGAAAAGTGGCAAAG
AATTCTCAACAGCCTGTGTCAGTTTTCTAACTTAATTGAAACACCTTTAAGCTCTCCTTC
ATCCCCAAACTTTCCCCTATGGTGTCTATGATAGAAAAACACTTCTCTCTCATATATGTC
CCATCACTAGGGAGCCAGGGGGAAGGGAGTGGGCATTACACAGGCAAGCCAAACTTTTTA
TTCAGCCAGAAAATGGATTTCTGATTCAGTTTAATGGTAAGGAGTTGTGTAAAACCGTAC
CAAGTCCTGCTTCTTCCTCAGAGTGAGCTGGGCTGCATTTTTGTGACTGGAAGCATTTCT
CATAATGGTGAATTAAGCACTATAAAAAAATAAAGTCAATCCCATCTCTATGAGTAGCAT
AAGAGCTGCTTCCAAGAACCCAGGGATGCCCACATCCCATAACCCACCCAGACACGGCCA
ATTCCTCATAGACAGCTACATAAAACCCTTTCGTCAACTTGTTTTGGGGGGTGAGAGAGA
GGCAGTGCTTANGGAAGATGTAGGATGGATTTTTTTTATTCCTGCCACTACCAAAACTAT
AAATCTCACACACACTCACACGCACATACATGTCCATTNTTAAGAAAGCTTTTCCTCCCT
GTGACTACNGAAACCCAGAATCCAGCCTCCCCTCAGCTGAGAGACGATGCCCTATTNACG
CACAGATCTAGCATATCAACATACTATAGAAGGGCTCTGTGA
Restriction Sites NotI-NotI |
ACCN NM_024102
Insert Size 2210 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024102.2, NP_077007.1
RefSeq Size 2428 bp
RefSeq ORF 1029 bp
Locus ID 79084
UniProt ID Q9BQA1
Cytogenetics 1p13.2
Domains WD40
Protein Families Stem cell - Pluripotency
Summary The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Nov 2015
Transcript Variant: This variant (2) uses an alternate in-frame splice junction in the 3' end compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.
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Documents
Resources
"NM_024102" in other vectors (6)
SKU Description Size Price
RC204532 WDR77 (Myc-DDK-tagged)-Human WD repeat domain 77 (WDR77) 10 ug
$457.00
RC204532L1 Lenti ORF clone of Human WD repeat domain 77 (WDR77), Myc-DDK-tagged 10 ug
$757.00
RC204532L2 Lenti ORF clone of Human WD repeat domain 77 (WDR77), mGFP tagged 10 ug
$757.00
RC204532L3 Lenti ORF clone of Human WD repeat domain 77 (WDR77), Myc-DDK-tagged 10 ug
$757.00
RC204532L4 Lenti ORF clone of Human WD repeat domain 77 (WDR77), mGFP tagged 10 ug
$757.00
RG204532 WDR77 (tGFP-tagged) - Human WD repeat domain 77 (WDR77) 10 ug
$657.00

file-alt Citations

PRMT5- mediated symmetric arginine dimethylation is attenuated by mutant huntingtin and is impaired in Huntington's Disease (HD)
Ratovitski, Arbez, Stewart et al
Cell Cycle (2015)
Cited by 74
AI Summary: OriGene products contributed human PRMT5 cDNA and human MEP50 cDNA subcloned into pcDNA expression vectors, which were used for ectopic expression studies to investigate their roles in Huntington's Disease pathogenesis.
Snippet: HttN511–8Q/52Q constructs were produced from N511–82Q by random contractions of polyQ repeat in bacterial cells. HttN586–20Q and Htt-N586– 82Q plasmids were described previously.18 Human PRMT5 cDNA subcloned into pcDNA expression vector was obtained from Origene. …
Showing 1-1 of 1 papers.
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