Claudin 2 (CLDN2) (NM_020384) Human Tagged ORF Clone
$489.00
$500.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Claudin 2 |
| Synonyms | OAZON |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_020384 |
| ORF Size | 690 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_020384.4 |
| RefSeq Size | 2959 bp |
| RefSeq ORF | 693 bp |
| Locus ID | 9075 |
| UniProt ID | P57739 |
| Cytogenetics | Xq22.3 |
| Protein Families | Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Tight junction |
| Summary | This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.provided by RefSeq, Jan 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_020384" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204199 | CLDN2 (Myc-DDK-tagged)-Human claudin 2 (CLDN2), transcript variant 1 | 10 ug |
$300.00
|
|
| RC204199L1 | Lenti ORF clone of Human claudin 2 (CLDN2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC204199L2 | Lenti ORF clone of Human claudin 2 (CLDN2), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RC204199L3 | Lenti ORF clone of Human claudin 2 (CLDN2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC204199L4 | Lenti ORF clone of Human claudin 2 (CLDN2), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| SC304755 | CLDN2 (untagged)-Human claudin 2 (CLDN2), transcript variant 1 | 10 ug |
$330.00
|
|
| SC320930 | CLDN2 (untagged)-Human claudin 2 (CLDN2), transcript variant 1 | 10 ug |
$300.00
|
|
Citations
Autophagy prevents ER stress-induced Tight Junction barrier disruption via claudin-2 homeostasis
Priya, Saha, Ganapathy et al
bioRxiv (2026)
Priya, Saha, Ganapathy et al
bioRxiv (2026)
AI Summary: OriGene’s CLDN2 ORF in pCMV6-AC-GFP plasmid (RG204199) was used to overexpress claudin-2 in Caco-2 cells, enabling the authors to study the effects of claudin-2 on ER stress and tight junction barrier function.
Snippet: he gene knockout was further confirmed using western blot analysis. CLDN2 ORF in pCMV6-AC-GFP (Origene, RG204199) and corresponding control plasmid were used to transfect Caco-2 cells using Lipofectamine 2000 (Invitrogen,
AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability
Ganapathy, Saha, Suchanec et al
Autophagy (2021)
Ganapathy, Saha, Suchanec et al
Autophagy (2021)
Cited by 97
Caveolin 1 is Associated with Upregulated Claudin 2 in Necrotizing Enterocolitis
Ares, Buonpane, Sincavage et al
Sci Rep (2019) 9 (1), 4982 DOI: 10.1038/s41598-019-41442-4
Ares, Buonpane, Sincavage et al
Sci Rep (2019) 9 (1), 4982 DOI: 10.1038/s41598-019-41442-4
AI Summary: OriGene’s Claudin 2 (CLDN2) Human Tagged ORF Clone (catalog #RG204199) was used to transfect cells, enabling the study of claudin 2’s role in intestinal barrier dysfunction in necrotizing enterocolitis.
Snippet: Lipofectamine 2000 (LifeTech, Elmhurst, IL) was used to transfect with the plasmid: Claudin 2 (CLDN2) (NM_020384) Human Tagged ORF Clone, transcript variant 1 (Origene, Rockville, MD, # RG204199) for 72 hours followed by stable selection with gentamycin
Claudin-2 Expression Levels in Ulcerative Colitis: Development and Validation of an In-Situ Hybridisation Assay for Therapeutic Studies
Randall, Henderson, Reens et al
PLoS ONE (2016) 11 (9), e0162076 DOI: 10.1371/journal.pone.0162076
Randall, Henderson, Reens et al
PLoS ONE (2016) 11 (9), e0162076 DOI: 10.1371/journal.pone.0162076
Cited by 21
AI Summary: OriGene’s human claudin-2 cDNA ORF clone (RG204199) was used to transiently transfect CHO-K1 cells, providing a positive control for validating claudin-2 detection assays in this study.
Snippet: CHO-K1 cells were cultured to approximately 50% confluency, in T175 flasks, prior to transiently transfecting with human claudin-2 cDNA ORF clone (RG204199, Origene) using Lipofectamine 2000 (11668027, Invitrogen) according to the manufacturer's instructions ... Cite SaveSaving
Showing 1-4 of 4 papers.
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