MAT1A (NM_000429) Human Tagged ORF Clone

  • TrueORF®
SKU
RG203765
MAT1A (tGFP-tagged) - Human methionine adenosyltransferase I, alpha (MAT1A)
$489.00 $657.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAT1A
Synonyms MAT; MATA1; SAMS; SAMS1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG203765 representing NM_000429
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGGACCGGTGGATGGCTTGTGTGACCACTCTCTAAGTGAAGGAGTCTTCATGTTCACATCGGAGT
CTGTGGGAGAGGGACACCCGGATAAGATCTGTGACCAGATCAGTGATGCAGTGCTGGATGCCCATCTCAA
GCAAGACCCCAATGCCAAGGTGGCCTGTGAGACAGTGTGCAAGACCGGCATGGTGCTGCTGTGTGGTGAG
ATCACCTCAATGGCCATGGTGGACTACCAGCGGGTGGTGAGGGACACCATCAAGCACATCGGCTACGATG
ACTCAGCCAAGGGCTTTGACTTCAAGACTTGCAACGTGCTGGTGGCTTTGGAGCAGCAATCCCCAGATAT
TGCCCAGTGCGTCCATCTGGACAGAAATGAGGAGGATGTGGGGGCAGGAGATCAGGGTTTGATGTTCGGC
TATGCCACCGACGAGACAGAGGAGTGCATGCCCCTCACCATCATCCTTGCTCACAAGCTCAACGCCCGGA
TGGCAGACCTCAGGCGCTCCGGCCTCCTCCCCTGGCTGCGGCCTGACTCTAAGACTCAGGTGACAGTTCA
GTACATGCAGGACAATGGCGCAGTCATCCCTGTGCGCATCCACACCATCGTCATCTCTGTGCAGCACAAC
GAAGACATCACGCTGGAGGAGATGCGCAGGGCCCTGAAGGAGCAAGTCATCAGGGCCGTGGTGCCGGCCA
AGTACCTGGACGAAGACACCGTCTACCACCTGCAGCCCAGTGGGCGGTTTGTCATCGGAGGTCCCCAGGG
GGATGCGGGTGTCACTGGCCGTAAGATTATTGTGGACACCTATGGCGGCTGGGGGGCTCATGGTGGTGGG
GCCTTCTCTGGGAAGGACTACACCAAGGTGGACCGCTCAGCCGCTTATGCTGCCCGCTGGGTGGCCAAGT
CTCTGGTGAAAGCAGGGCTCTGCCGGAGAGTGCTTGTCCAGGTTTCCTATGCCATTGGTGTGGCCGAGCC
GCTGTCCATTTCCATCTTCACCTACGGAACCTCTCAGAAGACAGAGCGAGAGCTGCTGGATGTGGTGCAT
AAGAACTTCGACCTCCGGCCGGGCGTCATTGTCAGGGATTTGGACTTGAAGAAGCCCATCTACCAGAAGA
CAGCATGCTACGGCCATTTCGGAAGAAGCGAGTTCCCATGGGAGGTTCCCAGGAAGCTTGTATTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG203765 representing NM_000429
Red=Cloning site Green=Tags(s)

MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGE
ITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFG
YATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHN
EDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGG
AFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVH
KNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000429
ORF Size 1185 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000429.2, NP_000420.1
RefSeq Size 3419 bp
RefSeq ORF 1188 bp
Locus ID 4143
UniProt ID Q00266
Cytogenetics 10q22.3
Domains S-AdoMet_synt
Protein Families Druggable Genome
Protein Pathways Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism
Summary This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. provided by RefSeq, Jul 2008
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_000429" in other vectors (4)
SKU Description Size Price
RC203765 MAT1A (Myc-DDK-tagged)-Human methionine adenosyltransferase I, alpha (MAT1A) 10 ug
$457.00
RC203765L3 Lenti ORF clone of Human methionine adenosyltransferase I, alpha (MAT1A), Myc-DDK-tagged 10 ug
$757.00
RC203765L4 Lenti ORF clone of Human methionine adenosyltransferase I, alpha (MAT1A), mGFP tagged 10 ug
$757.00
SC119881 MAT1A (untagged)-Human methionine adenosyltransferase I, alpha (MAT1A) 10 ug
$457.00

Plasmid Map for RG203765

Download sequence for

Cloning scheme for RG203765

Circular map for RG203765

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products