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MAT1A (NM_000429) Human Untagged Clone

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SKU
SC119881
MAT1A (untagged)-Human methionine adenosyltransferase I, alpha (MAT1A)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MAT1A
Synonyms MAT; MATA1; SAMS; SAMS1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC119881 sequence for NM_000429 edited (data generated by NextGen Sequencing)
ATGAATGGACCGGTGGATGGCTTGTGTGACCACTCTCTAAGTGAAGGAGTCTTCATGTTC
ACATCGGAGTCTGTGGGAGAGGGACACCCGGATAAGATCTGTGACCAGATCAGTGATGCA
GTGCTGGATGCCCATCTCAAGCAAGACCCCAATGCCAAGGTGGCCTGTGAGACAGTGTGC
AAGACCGGCATGGTGCTGCTGTGTGGTGAGATCACCTCAATGGCCATGGTGGACTACCAG
CGGGTGGTGAGGGACACCATCAAGCACATCGGCTACGATGACTCAGCCAAGGGCTTTGAC
TTCAAGACTTGCAACGTGCTGGTGGCTTTGGAGCAGCAATCCCCAGATATTGCCCAGTGC
GTCCATCTGGACAGAAATGAGGAGGATGTGGGGGCAGGAGATCAGGGTTTGATGTTCGGC
TATGCCACCGACGAGACAGAGGAGTGCATGCCCCTCACCATCATCCTTGCTCACAAGCTC
AACGCCCGGATGGCAGACCTCAGGCGCTCCGGCCTCCTCCCCTGGCTGCGGCCTGACTCT
AAGACTCAGGTGACAGTTCAGTACATGCAGGACAATGGCGCAGTCATCCCTGTGCGCATC
CACACCATCGTCATCTCTGTGCAGCACAACGAAGACATCACGCTGGAGGAGATGCGCAGG
GCCCTGAAGGAGCAAGTCATCAGGGCCGTGGTGCCGGCCAAGTACCTGGACGAAGACACC
GTCTACCACCTGCAGCCCAGTGGGCGGTTTGTCATCGGAGGTCCCCAGGGGGATGCGGGT
GTCACTGGCCGTAAGATTATTGTGGACACCTATGGCGGCTGGGGGGCTCATGGTGGTGGG
GCCTTCTCTGGGAAGGACTACACCAAGGTGGACCGCTCAGCCGCATATGCTGCCCGCTGG
GTGGCCAAGTCTCTGGTGAAAGCAGGGCTCTGCCGGAGAGTGCTTGTCCAGGTTTCCTAT
GCCATTGGTGTGGCCGAGCCGCTGTCCATTTCCATCTTCACCTACGGAACCTCTCAGAAG
ACAGAGCGAGAGCTGCTGGATGTGGTGCATAAGAACTTCGACCTCCGGCCGGGCGTCATT
GTCAGGGATTTGGACTTGAAGAAGCCCATCTACCAGAAGACAGCATGCTACGGCCATTTC
GGAAGAAGCGAGTTCCCATGGGAGGTTCCCAGGAAGCTTGTATTTTAG

Clone variation with respect to NM_000429.2
426 t=>c;870 a=>g;882 t=>c
5' Read Nucleotide Sequence
>OriGene 5' read for NM_000429 unedited
NGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGCTCAAAATGTTCACTGGAA
AAAAGCGTGAGTGGAGAAGTGTGAGAAGATGAATGGACCGGTGGATGGCTTGTGTGACCA
CTCTCTAAGTGAAGGAGTCTTCATGTTCACATCGGAGTCTGTGGGAGAGGGACACCCGGA
TAAGATCTGTGACCAGATCAGTGATGCAGTGCTGGATGCCCATCTCAAGCAAGACCCCAA
TGCCAAGGTGGCCTGTGAGACAGTGTGCAAGACCGGCATGGTGCTGCTGTGTGGTGAGAT
CACCTCAATGGCCATGGTGGACTACCAGCGGGTGGTGAGGGACACCATCAAGCACATCGG
CTACGATGACTCAGCCAAGGGCTTTGACTTCAAGACTTGCAACGTGCTGGTGGCTTTGGA
GCAGCAATCCCCAGATATTGCCCAGTGCGTCCATCTGGACAGAAATGAGGAGGATGTGGG
GGCAGGAGATCAGGGTTTGATGTTCGGCTATGCCACCGACGAGACAGAGGAGTGCATGCC
CCTCACCATCATCCTTGCTCACAAGCTCAACGCCCGGATGGCAGACCTCANGCGCTTCGG
CCTCCTNCCCTGGCTGCGGCCTGACTCTAAGACTCANGTGACAGTTCAGTACATGCAGGA
CATGGGCGCAGTCATCCCTGTGCGCATNCACACATCGTCATCTCTGTGCAGCACAACGAA
GACATCACGCTGGNAGAGATGCGCANGGCCCCTGAGGAGCAGTCATCGGGCCCGTGGNTG
CCGNCCAGTACCTGNACGAAGACACCGTCTACCCCTGCAGCCAGTGGNGCGGTTNGTCAT
CGGAGGTCCCAGGGGATGGCGGNTGTCACTGCCGTAGAATATTGTGGACACTATGGCGGC
TGGGGCTATTGGGGGGGTGGGGCTCTNCTGGAAGGACTACACAAGTGGACCGTCACCGCT
ATCTGCCCCTGGTGGCAGTCTTGNGN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_000429 unedited
NAAGGTTTTTACTNTGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTT
TTTAGTTGCTCCTTGAGTTTTTATTCATTGTTTTAGCAGGAACTATTAACAAATAAAGGC
TTTAGTTTCATACAAACAAACTGACAAAAAAGATCTTATTTCTCTAGAGGGTAGGGAAAG
TATAAAATTCTGAATTTTCATGTCGAGTGTGAGCCAAGTTAGAGGAACTTGGCCACCTGC
AAACCACTCCCTCCCTCCATGGGAAGGAATCTGAGGCTTCCTAGGTGACCAGGAGCCGGG
CTTCTTTTGTTGCCTTAATTTCCTTTCACCTGAGAGAAAATGAAAGCCAGGGTCTTTGTG
CTCCAGGCCAAGGCCCAGATGCTGGCTGCCTGCTCCCCAGATGAGACTTCCTGGGCTGCC
TCTCCCAGCACCATGTACCCTAGAGAGAGGCCAAGTCCCCAGTGTTCCCCTGGGGCCCAT
GGAGGCCCCTGCCTGAGCCTGTGGTCCTGGGAGCTGGGGAGAGGTANAGATGCTGCTGTC
AGAAGTCCAGGCTAAGGACAAGTGGGGCANGTANGGCANGTGCTGCTGGCCATGGATGGA
AAGGCANNGCAGTTTCCAGACCTTAGCANGCCAAGGACTGCTCANGCCCCTTGGGAGCAG
ACCCGCTCCCAGACAGTACCTGGNCAAAGCAGAATCTNCTCATGACGTCTCAAAGCCTGT
CCGACACCAGAAGGCAGCCCCGCCTATTTACATGAGGCCCATAGACCAGCCTNCCCTGAT
CCCGAAAGGGCACTCACTTCCTTCAATGCCTCAGNCTGCAGGGGATACAGGGACCANNGA
AAGAGAAAGACTGAGGCCTCTGTCTAGGGNGCTGCCTNTGTGCTGGAACAAGTGCTGCTC
TGCTCCAGAGCCAGATAGACTGGACTGACT
Restriction Sites NotI-NotI |
ACCN NM_000429
Insert Size 3520 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000429.2, NP_000420.1
RefSeq Size 3419 bp
RefSeq ORF 1188 bp
Locus ID 4143
UniProt ID Q00266
Cytogenetics 10q22.3
Domains S-AdoMet_synt
Protein Families Druggable Genome
Protein Pathways Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism
Summary This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_000429" in other vectors (4)
SKU Description Size Price
RC203765 MAT1A (Myc-DDK-tagged)-Human methionine adenosyltransferase I, alpha (MAT1A) 10 ug
$457.00
RC203765L3 Lenti ORF clone of Human methionine adenosyltransferase I, alpha (MAT1A), Myc-DDK-tagged 10 ug
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RC203765L4 Lenti ORF clone of Human methionine adenosyltransferase I, alpha (MAT1A), mGFP tagged 10 ug
$757.00
RG203765 MAT1A (tGFP-tagged) - Human methionine adenosyltransferase I, alpha (MAT1A) 10 ug
$489.00 $657.00

file-alt Citations

Methionine Adenosyltransferase 1a (MAT1A) Enhances Cell Survival During Chemotherapy Treatment and is Associated with Drug Resistance in Bladder Cancer PDX Mice
Martin, Hum, Sebastian et al
Int J Mol Sci (2019) 20 (20) DOI: 10.3390/ijms20204983
AI Summary: In this study, OriGene's MAT1A cDNA clone (RC200774) was used to overexpress MAT1A in bladder cancer cells, enabling the researchers to demonstrate that increased MAT1A levels enhance cell survival and drug resistance during chemotherapy.
Snippet: … Our work strengthens this observation and provides new mechanistic evidence that alterations of this pathway directly affect drug tolerance to gemcitabine and contributes to the emergence of drug relapse in bladder cancer patient derived xenograft models. Here we show that elevated levels of MAT1A
Human liver methionine cycle: MAT1A and GNMT gene resequencing, functional genomics, and hepatic genotype-phenotype correlation
Ji, Nordgren, Chai et al
Drug Metab. Dispos. (2012) 40 (10), 1984-92 DOI: 10.1124/dmd.112.046953
Cited by 16
AI Summary: OriGene’s MAT1A clone (SC119881) was used to generate mammalian expression constructs for functional studies of gene variants in this paper.
Snippet: Mammalian expression constructs were created for wild-type (WT) MAT1A by subcloning the open reading frame of MAT1A from the Origene clone SC119881 (Origene, Rockville, MD) into pcDNA3.1D/V5-His-TOPO (Invitrogen, Carlsbad, CA) in frame with the V5-His tag, and
Showing 1-2 of 2 papers.
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