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CENTB1 (ACAP1) (NM_014716) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG203724
ACAP1 (tGFP-tagged) - Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$789.00 $878.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CENTB1
Synonyms CENTB1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG203724 representing NM_014716
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGTCAAGCTGGATTTCGAGGAGTGTCTCAAGGACTCACCCCGTTTCCGAGCCTCTATTGAGCTGG
TGGAAGCCGAAGTGTCAGAATTGGAGACCCGTCTGGAAAAGCTCCTGAAACTGGGCACTGGTCTCCTGGA
AAGTGGGCGCCATTACCTTGCTGCCAGCCGCGCCTTCGTTGTCGGCATTTGTGACCTGGCCCGCCTGGGT
CCACCAGAGCCCATGATGGCGGAGTGTCTGGAAAAATTCACCGTGAGCCTGAACCACAAGCTGGACAGCC
ATGCGGAGCTTCTAGATGCCACCCAACACACACTGCAGCAGCAGATCCAGACCCTGGTCAAGGAAGGTCT
GCGGGGTTTCCGAGAGGCTCGCCGGGATTTCTGGCGGGGGGCTGAGAGCCTGGAGGCTGCCCTGACCCAC
AACGCAGAGGTTCCCAGGCGCCGGGCCCAGGAGGCAGAAGAGGCAGGAGCTGCTTTGAGGACGGCTCGAG
CTGGGTACCGGGGACGGGCACTGGATTATGCCCTGCAGATCAACGTGATTGAGGACAAGAGGAAGTTTGA
CATCATGGAGTTTGTGCTGCGTTTGGTGGAGGCCCAGGCTACCCATTTCCAGCAGGGCCATGAGGAGCTG
AGCCGGCTGTCCCAGTATCGAAAGGAGCTGGGCGCCCAGTTGCACCAGCTGGTCTTGAATTCAGCACGAG
AGAAGAGGGACATGGAGCAGAGACACGTGCTGCTGAAACAGAAGGAGCTGGGTGGGGAGGAGCCAGAACC
AAGCTTAAGAGAGGGGCCTGGTGGCCTGGTGATGGAAGGACATCTCTTCAAACGGGCCAGCAACGCATTT
AAGACCTGGAGCAGACGCTGGTTCACCATTCAGAGCAACCAACTGGTTTACCAGAAGAAGTACAAGGACC
CTGTGACTGTGGTGGTGGATGACCTTCGTCTCTGCACAGTGAAACTCTGCCCTGACTCAGAAAGGCGGTT
CTGCTTTGAGGTGGTGTCCACCAGCAAGTCCTGCCTCCTCCAGGCTGACTCAGAGCGCCTCCTGCAGCTG
TGGGTCAGTGCTGTGCAGAGCAGCATTGCTTCTGCCTTCAGTCAGGCTCGCCTTGATGACAGCCCCCGGG
GTCCAGGCCAGGGCTCAGGACACCTGGCCATAGGCTCTGCTGCCACCCTGGGCTCTGGTGGAATGGCCAG
GGGAAGGGAGCCTGGGGGAGTCGGGCACGTGGTGGCCCAGGTCCAGAGTGTGGATGGCAATGCCCAGTGC
TGCGACTGCCGGGAGCCAGCCCCGGAGTGGGCCAGCATCAACCTTGGTGTCACCCTCTGCATTCAGTGTT
CCGGCATCCACAGGAGCCTTGGTGTTCACTTCTCCAAAGTCCGGTCTCTGACCCTTGACTCATGGGAGCC
AGAACTAGTGAAGCTCATGTGTGAGCTGGGAAATGTCATCATCAACCAGATCTATGAGGCCCGCGTGGAG
GCCATGGCAGTGAAGAAACCAGGGCCCAGCTGCTCCCGGCAGGAGAAGGAGGCCTGGATTCACGCTAAAT
ACGTGGAGAAGAAGTTCCTGACCAAGCTGCCTGAGATTCGAGGGCGAAGAGGTGGCCGGGGGCGCCCAAG
GGGGCAGCCTCCTGTGCCCCCAAAGCCTTCCATCAGGCCCCGGCCAGGGAGCTTGAGATCCAAGCCAGAG
CCCCCCTCTGAGGACCTGGGAAGCCTGCACCCTGGGGCCCTACTGTTTCGAGCGTCTGGGCATCCTCCAT
CTCTTCCCACCATGGCTGATGCCCTTGCCCATGGAGCTGATGTCAACTGGGTCAATGGGGGCCAAGATAA
TGCCACACCGCTGATCCAGGCCACAGCTGCTAATTCTCTTCTGGCCTGTGAGTTTCTCCTCCAGAACGGG
GCGAACGTGAACCAAGCGGACAGTGCGGGCCGGGGCCCGCTGCACCACGCAACCATTCTTGGCCACACGG
GGCTCGCCTGCCTGTTCCTGAAACGGGGAGCTGATCTGGGGGCTCGAGACTCTGAAGGCAGGGACCCTCT
GACCATCGCCATGGAAACAGCCAACGCTGACATCGTCACCCTGCTACGACTGGCAAAGATGAGGGAGGCT
GAAGCGGCCCAGGGGCAGGCAGGAGATGAGACGTATCTTGACATCTTCCGCGACTTCTCCCTCATGGCGT
CAGACGACCCGGAGAAGCTGAGCCGTCGCAGTCATGACCTCCACACGCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG203724 representing NM_014716
Red=Cloning site Green=Tags(s)

MTVKLDFEECLKDSPRFRASIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLG
PPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTH
NAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEEL
SRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELGGEEPEPSLREGPGGLVMEGHLFKRASNAF
KTWSRRWFTIQSNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCLLQADSERLLQL
WVSAVQSSIASAFSQARLDDSPRGPGQGSGHLAIGSAATLGSGGMARGREPGGVGHVVAQVQSVDGNAQC
CDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVE
AMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGRPRGQPPVPPKPSIRPRPGSLRSKPE
PPSEDLGSLHPGALLFRASGHPPSLPTMADALAHGADVNWVNGGQDNATPLIQATAANSLLACEFLLQNG
ANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDSEGRDPLTIAMETANADIVTLLRLAKMREA
EAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014716
ORF Size 2220 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014716.4
RefSeq Size 2498 bp
RefSeq ORF 2223 bp
Locus ID 9744
UniProt ID Q15027
Cytogenetics 17p13.1
Domains ANK, ArfGap, PH
Protein Pathways Endocytosis
Summary GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.UniProtKB/Swiss-Prot Function
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"NM_014716" in other vectors (5)
SKU Description Size Price
RC203724 ACAP1 (Myc-DDK-tagged)-Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1) 10 ug
$678.00
RC203724L3 Lenti ORF clone of Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1), Myc-DDK-tagged 10 ug
$978.00
RC203724L4 Lenti ORF clone of Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1), mGFP tagged 10 ug
$978.00
SC114871 ACAP1 (untagged)-Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1) 10 ug
$679.00
SC322463 ACAP1 (untagged)-Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1) 10 ug
$679.00

Plasmid Map for RG203724

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG203724
Circular map for RG203724

file-alt Citations

A Brucella effector modulates the Arf6-Rab8a GTPase cascade to promote intravacuolar replication
Borghesan, Smith, Myeni et al
EMBO J (2021)
Cited by 21
AI Summary: OriGene’s pCMV6-AC-GFP-ACAP1 plasmid (Cat# RG203724) was used as the source of human ACAP1 cDNA for cloning and expression studies that helped elucidate the interaction between the Brucella effector BspF and ACAP1 in modulating host membrane transport.
Snippet: Human ACAP1 cDNA was originally amplified from pCMV6-AC-GFP-ACAP1 (OriGene #RG203724) using primers RC451 and RC452 and cloned into pCMV-myc (Clontech) using the EcoRI restriction site to generate pCMV-myc-ACAP1
Showing 1-1 of 1 papers.
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