CENTB1 (ACAP1) (NM_014716) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC203724L4
Lenti ORF clone of Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1), mGFP tagged
  $978.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CENTB1
Synonyms CENTB1
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC203724).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014716
ORF Size 2220 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014716.2
RefSeq Size 2523 bp
RefSeq ORF 2223 bp
Locus ID 9744
UniProt ID Q15027
Cytogenetics 17p13.1
Domains ANK, ArfGap, PH
Protein Pathways Endocytosis
MW 81.5 kDa
Summary GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_014716" in other vectors (5)
SKU Description Size Price
RC203724 ACAP1 (Myc-DDK-tagged)-Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1) 10 ug
$678.00
RC203724L3 Lenti ORF clone of Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1), Myc-DDK-tagged 10 ug
$978.00
RG203724 ACAP1 (tGFP-tagged) - Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1) 10 ug
$789.00 $878.00
SC114871 ACAP1 (untagged)-Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1) 10 ug
$679.00
SC322463 ACAP1 (untagged)-Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1) 10 ug
$679.00

Plasmid Map for RC203724L4

Download sequence for

Cloning scheme for RC203724L4

Circular map for RC203724L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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