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EEF1AKMT3 (NM_015433) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG203680
METTL21B (tGFP-tagged) - Human methyltransferase like 21B (METTL21B), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $500.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EEF1AKMT3
Synonyms FAM119B; METTL21B
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG203680 representing NM_015433
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACCCCGGCCCAGATCCCGAATCTGAGTCGGAATCGGTGTTCCCGCGGGAGGTCGGGCTCTTTG
CAGACTCTTACTCGGAGAAGAGCCAGTTCTGTTTCTGTGGGCATGTGCTGACCATCACGCAGAACTTTGG
GTCCCGCCTCGGGGTGGCGGCGCGCGTGTGGGACGCGGCCCTGAGCCTGTGCAATTATTTCGAGAGTCAA
AATGTGGATTTCCGAGGCAAGAAGGTGATCGAACTGGGTGCGGGGACAGGCATCGTGGGGATCTTGGCAG
CGCTGCAGGGGGGGGATGTTACCATCACTGACCTGCCCCTGGCCCTAGAACAGATCCAGGGCAACGTCCA
GGCCAATGTGCCAGCTGGAGGCCAGGCCCAGGTGCGTGCCTTGTCCTGGGGGATTGACCATCATGTCTTC
CCTGCAAACTATGACCTGGTGCTGGGGGCTGATATCGTGTACCTGGAACCCACCTTCCCTCTGCTGCTGG
GGACCCTCCAACACCTGTGCAGGCCCCATGGCACCATCTATCTGGCCTCCAAGATGAGAAAGGAGCATGG
GACAGAGAGCTTCTTTCAGCACCTCCTGCCCCAGCATTTCCAACTGGAGCTGGCTCAGCGGGATGAGGAT
GAAAATGTCAACATCTATAGGGCCAGGCACAGGGAACCAAGACCTGCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG203680 representing NM_015433
Red=Cloning site Green=Tags(s)

MADPGPDPESESESVFPREVGLFADSYSEKSQFCFCGHVLTITQNFGSRLGVAARVWDAALSLCNYFESQ
NVDFRGKKVIELGAGTGIVGILAALQGGDVTITDLPLALEQIQGNVQANVPAGGQAQVRALSWGIDHHVF
PANYDLVLGADIVYLEPTFPLLLGTLQHLCRPHGTIYLASKMRKEHGTESFFQHLLPQHFQLELAQRDED
ENVNIYRARHREPRPA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015433
ORF Size 678 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015433.3
RefSeq Size 2717 bp
RefSeq ORF 681 bp
Locus ID 25895
UniProt ID Q96AZ1
Cytogenetics 12q14.1
Summary Protein-lysine methyltransferase that selectively methylates EEF1A1 and EEF1A2 at 'Lys-165' in an aminoacyl-tRNA and GTP-dependent manner. EEF1A1 methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation.UniProtKB/Swiss-Prot Function
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"NM_015433" in other vectors (4)
SKU Description Size Price
RC203680 METTL21B (Myc-DDK-tagged)-Human methyltransferase like 21B (METTL21B), transcript variant 1 10 ug
$300.00
RC203680L3 Lenti ORF clone of Human methyltransferase like 21B (METTL21B), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC203680L4 Lenti ORF clone of Human methyltransferase like 21B (METTL21B), transcript variant 1, mGFP tagged 10 ug
$600.00
SC126219 METTL21B (untagged)-Human methyltransferase like 21B (METTL21B), transcript variant 1 10 ug
$300.00

Plasmid Map for RG203680

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RG203680
Circular map for RG203680

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