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EEF1AKMT3 (NM_015433) Human Untagged Clone

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SKU
SC126219
METTL21B (untagged)-Human methyltransferase like 21B (METTL21B), transcript variant 1
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EEF1AKMT3
Synonyms FAM119B; METTL21B
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_015433 edited
CTCAGGGAGGCGGGGCTCGTTCCACAGGGACACCACGACGGCTCGCGGCCCCCAGCCTCT
ACCCCGCTCCGGATCCGGGATCTGAGCGCCGGCCGCGGTGCCCAGGCACTCCCTTGGCGG
GCCGGATGGCGGACCCCGGCCCAGATCCCGAATCTGAGTCGGAATCGGTGTTCCCGCGGG
AGGTCGGGCTCTTTGCAGACTCTTACTCGGAGAAGAGCCAGTTCTGTTTCTGTGGGCATG
TGCTGACCATCACGCAGAACTTTGGGTCCCGCCTCGGGGTGGCGGCGCGCGTGTGGGACG
CGGCCCTGAGCCTGTGCAATTATTTCGAGAGTCAAAATGTGGATTTCCGAGGCAAGAAGG
TGATCGAACTGGGTGCGGGGACAGGCATCGTGGGGATCTTGGCAGCGCTGCAGGGGGGGG
ATGTTACCATCACTGACCTGCCCCTGGCCCTAGAACAGATCCAGGGCAACGTCCAGGCCA
ATGTGCCAGCTGGAGGCCAGGCCCAGGTGCGTGCCTTGTCCTGGGGGATTGACCATCATG
TCTTCCCTGCAAACTATGACCTGGTGCTGGGGGCTGATATCGTGTACCTGGAACCCACCT
TCCCTCTGCTGCTGGGGACCCTCCAACACCTGTGCAGGCCCCATGGCACCATCTATCTGG
CCTCCAAGATGAGAAAGGAGCATGGGACAGAGAGCTTCTTTCAGCACCTCCTGCCCCAGC
ATTTCCAACTGGAGCTGGCTCAGCGGGATGAGGATGAAAATGTCAACATCTATAGGGCCA
GGCACAGGGAACCAAGACCTGCTTGACATCACCCTTGCTGTTCTTCTCAATCTCTTGCTC
TAATGAAGGAACTGTGTATCTCAAAAACCATATTTCCAGAGCCACAAACATGAGGACCAA
AAAGGATGGATTTCCCTGGCCTCTCTCACTTTCCTCCTTCCCTCTTTGTTACCCCAGATA
CTCTTGGGCAGGTGCAGTGAGGTGCCTGCTTACAAGGAATCCCAGAGTTCTGGCAGCACT
AGTGTTAGAACACCAAGGAGGTTCCTGGCTCCTGTTCTCAGAGAAGGAGGATGGTAAGGT
ATCCAGGATTTTTAGGGGGTAAGGGAGATATATGGGATGTGAGAGCAGTGATTGTAGACA
GACTGTCACTGATCACTTCCATTCCTGTTGCTGTTTACACAGAATGGACTTTAAAAAAAT
TCTGTGGGATTTTTTTTTCTTTTTTTCTTTTTTTTTGAGACAGGGTCTCACTCTGTCATC
CAGGCTGGAGTGCAGTGGTGTGATCACAGCTCACTGCAGCCTCAACCTCTGAAGGCCCAG
ATGATCTTCCTGCCTCAGCTCCCCAAGTAGCTGGGACTAGAGGCATGCGCCACCACACCC
GGCTAATTTTTGTATTTTCAGTAGAGACAGGGTTTCGCCATGTTGCCCAGGGTGGTCTCA
AACTCCTGGGCTCAAGTGATCCACCCGCCTTGCCTCCCAAAGTGCTGGGATTGCAGGCAT
GGCCCACTGTGCTAGCCTAGGATTTTTTTTTTTTTTTAAGAAAGAATTAAGCCTTAAAAA
TCTATTGTGCAAGAACCCAACTTTTACTCAGGAGCTGAGAAGGAAGAAGCCTCAAACCAC
CCATTTCCTGCTTTTTAGAGATAGAGTCTAGTGGAACCATGGCTCTAACTGGGTCAAGTC
CTCCTAGCTCTGAAGCTAAGGCTGCAGAGCTGTCCTTTGGACTGTATTTCTTCATGCTTT
CCAGAGTTAGAGCCTTGATCACGGAACATCATTGTGTGCAGAAGAATACTGTACCTAGTA
CTAAGTTCTTTTCATCTTTGATTTTTCTTTAGCTTCATCAGTCCTTCCTTCCTAATCTCT
AATCCCCTGAAGCCTAACACAGTTCCTAATGTGTTTTGCCTTAACCTCTTTTCTCTTCCC
ACATTGGTACTTCTGGTTGGGGCTCTCTTTACTTACTGCTTAGGCACATAGTCACCTTCT
CTGGACTCCCTGCTTCCATGCTTTCTCCTTCAGTCCATTCCGCAAACTCTTGTGATCTTC
CAAAATTAGCTCTGATTAGCTTTCACTTCCTGTAGAAGCAAGAACAAGCTCCTCTGCTTG
GTACTTAGTCTGTGTCCATGTGATCCAAAATTACCTTCCTATTGCATTTCCTACTCCTTT
GCTACGTGTATTGGACCTCTGGCCAAATTACAAAAGATGTACTCTTTGTTCTCTAAAACC
ACACTAAAGTTTTCTGACTTCTTTCACACTCTTTATGATAATCCTCTGCCTGAAAGGCTA
TTGCCACCTGTCAAAATCTTCATTATTCTTTGCCTCTTTCCTGAGCCTCTTGCTTGAATG
TGATTTCTTTCTCCCTGAGACCCATAAGGTTCATCTTATGGGACCTTAATTTGTCTTGCA
TGGACATTTGTGATTCTCATTTTCTCACCATTAAATTACCTTGGAGGGTAGGGCTGTGGT
TTACTCCTGTCTAAACCCCTGAAGCCTAACACAGTTCCTGGCACACAACAGGTGTTCAAC
AGATATTGTGCTCATTCAATAAATATTTACTCGCTTTGTTTAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_015433 unedited
GGTTTAGATTTGTAAACGACTTATATAGGCGAGCCGCGAATCAAATCTGGTACCGGTCCG
GAATTCCCGGGATATCGTCGACCCACGCGTCCGCTCAGGGAGGCGGGGCTCGTTCCACAG
GGACACCACGACGGCTCGCGGCCCCCAGCCTCTACCCCGCTCCGGATCCGGGATCTGAGC
GCCGGCCGCGGTGCCCAGGCACTCCCTTGGCGGGCCGGATGGCGGACCCCGGCCCAGATC
CCGAATCTGAGTCGGAATCGGTGTTCCCGCGGGAGGTCGGGCTCTTTGCAGACTCTTACT
CGGAGAAGAGCCAGTTCTGTTTCTGTGGGCATGTGCTGACCATCACGCAGAACTTTGGGT
CCCGCCTCGGGGTGGCGGCGCGCGTGTGGGACGCGGCCCTGAGCCTGTGCAATTATTTCG
AGAGTCAAAATGTGGATTTCCGAGGCAAGAAGGTGATCGAACTGGGTGCGGGGACAGGCA
TCGTGGGGATCTTGGCAGCGCTGCAGGGGGGGGATGTTACCATCACTGACCTGCCCCTGG
CCCTAGAACAGATCCAGGGCAACGTCCAGGCCAATGTGCCAGCTGGAGGCCAGGCCCAGG
TGCGTGCCTTGTCCTGNGGGATTGACCATCATGTCTTCCCTGCAAACTATGACCTGGTGC
TGGGGGCTGATATCGTGTACCTGGAACCCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC
ACCTGTGCAGGCCCCATGGCACCATCTATCTGGCCTCCAAGATGAGAAAGGAGCATGGGA
CAGAGAGCTTCTTTCAGCACCTCCTGCCCCAGCATTTCCAACTGGAGCTGGCTCACCGGG
ATGAAGATGAAAATGTCAACATCTATAGGGCCAGGCACAGGGAACCAAGACCTGCTTGAC
ATCACCCTT
Restriction Sites NotI-NotI |
ACCN NM_015433
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015433.2, NP_056248.2
RefSeq Size 2717 bp
RefSeq ORF 681 bp
Locus ID 25895
UniProt ID Q96AZ1
Cytogenetics 12q14.1
Summary Protein-lysine methyltransferase that selectively methylates EEF1A1 and EEF1A2 at 'Lys-165' in an aminoacyl-tRNA and GTP-dependent manner. EEF1A1 methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longer isoform (a).
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Documents
Resources
"NM_015433" in other vectors (4)
SKU Description Size Price
RC203680 METTL21B (Myc-DDK-tagged)-Human methyltransferase like 21B (METTL21B), transcript variant 1 10 ug
$300.00
RC203680L3 Lenti ORF clone of Human methyltransferase like 21B (METTL21B), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC203680L4 Lenti ORF clone of Human methyltransferase like 21B (METTL21B), transcript variant 1, mGFP tagged 10 ug
$600.00
RG203680 METTL21B (tGFP-tagged) - Human methyltransferase like 21B (METTL21B), transcript variant 1 10 ug
$500.00

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