IMPDH2 (NM_000884) Human Tagged ORF Clone
- TrueORF®
SKU
RG202977
IMPDH2 (tGFP-tagged) - Human IMP (inosine 5'-monophosphate) dehydrogenase 2 (IMPDH2)
$489.00
$679.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | IMPDH2 |
| Synonyms | IMPD2; IMPDH-II |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000884 |
| ORF Size | 1542 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000884.3 |
| RefSeq Size | 1712 bp |
| RefSeq ORF | 1545 bp |
| Locus ID | 3615 |
| UniProt ID | P12268 |
| Cytogenetics | 3p21.31 |
| Domains | CBS, IMPDH |
| Protein Families | Druggable Genome |
| Protein Pathways | Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism |
| Summary | This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000884" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202977 | IMPDH2 (Myc-DDK-tagged)-Human IMP (inosine 5'-monophosphate) dehydrogenase 2 (IMPDH2) | 10 ug |
$479.00
|
|
| RC202977L1 | Lenti ORF clone of Human IMP (inosine 5'-monophosphate) dehydrogenase 2 (IMPDH2), Myc-DDK-tagged | 10 ug |
$779.00
|
|
| RC202977L2 | Lenti ORF clone of Human IMP (inosine 5'-monophosphate) dehydrogenase 2 (IMPDH2), mGFP tagged | 10 ug |
$779.00
|
|
| RC202977L3 | Lenti ORF clone of Human IMP (inosine 5'-monophosphate) dehydrogenase 2 (IMPDH2), Myc-DDK-tagged | 10 ug |
$779.00
|
|
| RC202977L4 | Lenti ORF clone of Human IMP (inosine 5'-monophosphate) dehydrogenase 2 (IMPDH2), mGFP tagged | 10 ug |
$779.00
|
|
| SC119585 | IMPDH2 (untagged)-Human IMP (inosine 5'-monophosphate) dehydrogenase 2 (IMPDH2) | 10 ug |
$479.00
|
|
Citations
Assembly of IMPDH2-based, CTPS-based, and mixed rod/ring structures is dependent on cell type and conditions of induction
Keppeke, Calise, Chan et al
J Genet Genomics (2015) 42 (6), 287-99 DOI: 10.1016/j.jgg.2015.04.002
Keppeke, Calise, Chan et al
J Genet Genomics (2015) 42 (6), 287-99 DOI: 10.1016/j.jgg.2015.04.002
Cited by 61
AI Summary: In this study, OriGene products—specifically the NHA-CTPS1 construct—were used to transfect cells and investigate the role of CTPS1 in the formation of rod/ring structures under various induction conditions.
Assembly of IMPDH2-Based, CTPS-Based, and Mixed Rod/Ring Structures Is Dependent on Cell Type and Conditions of Induction
Keppeke, Calise, Chan
Journal of Genetics and Genomics (2015)
Keppeke, Calise, Chan
Journal of Genetics and Genomics (2015)
Microinjection of specific anti-IMPDH2 antibodies induces disassembly of cytoplasmic rods/rings that are primarily stationary and stable structures
Keppeke, Andrade, Grieshaber et al
Cell Biosci (2015) 5 (1), 1 DOI: 10.1186/2045-3701-5-1
Keppeke, Andrade, Grieshaber et al
Cell Biosci (2015) 5 (1), 1 DOI: 10.1186/2045-3701-5-1
Cited by 83
AI Summary: OriGene products contributed to this study by providing the GFP-tagged IMPDH2 plasmid (RG208442), which was used to transfect COS-7 and HeLa cells and visualize the dynamics and disassembly of RR structures upon microinjection of anti-IMPDH2 antibodies.
Induction of cytoplasmic rods and rings structures by inhibition of the CTP and GTP synthetic pathway in mammalian cells
Carcamo, Satoh, Kasahara et al
PLoS One (2011) 6 (12), e29690 DOI: 10.1371/journal.pone.0029690
Carcamo, Satoh, Kasahara et al
PLoS One (2011) 6 (12), e29690 DOI: 10.1371/journal.pone.0029690
Cited by 213
AI Summary: OriGene products contributed to this study by providing the IMPDH2 shRNA (OriGene, Cat# TG310327) for gene knockdown experiments, which helped demonstrate that IMPDH2 depletion enhanced rods and rings (RR) formation upon Ribavirin treatment.
Showing 1-4 of 4 papers.
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