PEX7 (NM_000288) Human Tagged ORF Clone

  • TrueORF®
SKU
RG202432
PEX7 (tGFP-tagged) - Human peroxisomal biogenesis factor 7 (PEX7)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PEX7
Synonyms PBD9B; PTS2R; RCDP1; RD
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG202432 representing NM_000288
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGCGGTGTGCGGTGGAGCGGCGCGGATGCTGCGGACGCCGGGACGCCACGGCTACGCCGCCGAGT
TCTCCCCGTACCTGCCGGGCCGCCTGGCCTGCGCCACCGCGCAGCACTACGGCATCGCGGGCTGTGGAAC
CCTACTAATATTGGATCCAGATGAAGCTGGGCTAAGGCTTTTTAGAAGCTTTGACTGGAATGATGGTTTG
TTTGATGTGACTTGGAGTGAGAACAACGAACATGTCCTCATCACCTGTAGTGGCGATGGCTCGCTGCAGC
TCTGGGACACTGCCAAAGCTGCAGGGCCACTGCAAGTCTATAAAGAACACGCTCAGGAGGTGTATAGTGT
TGATTGGAGCCAAACCAGAGGTGAACAGCTTGTGGTGTCTGGCTCATGGGATCAAACTGTCAAATTGTGG
GATCCAACTGTTGGAAAGTCTCTGTGCACCTTTAGAGGCCATGAAAGTATTATTTATAGCACAATCTGGT
CTCCCCACATCCCTGGTTGTTTTGCTTCAGCCTCAGGTGATCAGACTCTGAGAATATGGGATGTGAAGGC
AGCAGGAGTAAGAATCGTGATTCCTGCACATCAGGCAGAAATCTTGAGTTGTGACTGGTGTAAATACAAT
GAGAATTTGCTGGTGACCGGGGCGGTTGACTGTAGTTTGAGAGGCTGGGACTTAAGGAATGTACGACAAC
CAGTGTTTGAACTTCTTGGTCATACCTATGCTATTAGGAGGGTGAAATTTTCACCATTTCATGCTTCTGT
GCTGGCCTCTTGCTCGTATGATTTTACTGTAAGATTCTGGAACTTTTCAAAGCCTGACTCTCTTCTTGAA
ACAGTGGAGCATCATACAGAGTTTACTTGTGGTTTAGACTTCAGTCTTCAGAGCCCCACTCAGGTGGCTG
ACTGTTCTTGGGATGAAACAATAAAGATCTATGACCCTGCTTGTCTTACTATTCCTGCT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG202432 representing NM_000288
Red=Cloning site Green=Tags(s)

MSAVCGGAARMLRTPGRHGYAAEFSPYLPGRLACATAQHYGIAGCGTLLILDPDEAGLRLFRSFDWNDGL
FDVTWSENNEHVLITCSGDGSLQLWDTAKAAGPLQVYKEHAQEVYSVDWSQTRGEQLVVSGSWDQTVKLW
DPTVGKSLCTFRGHESIIYSTIWSPHIPGCFASASGDQTLRIWDVKAAGVRIVIPAHQAEILSCDWCKYN
ENLLVTGAVDCSLRGWDLRNVRQPVFELLGHTYAIRRVKFSPFHASVLASCSYDFTVRFWNFSKPDSLLE
TVEHHTEFTCGLDFSLQSPTQVADCSWDETIKIYDPACLTIPA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000288
ORF Size 969 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000288.4
RefSeq Size 1451 bp
RefSeq ORF 972 bp
Locus ID 5191
UniProt ID O00628
Cytogenetics 6q23.3
Domains WD40
Protein Families Druggable Genome
Summary This gene encodes the cytosolic receptor for the set of peroxisomal matrix enzymes targeted to the organelle by the peroxisome targeting signal 2 (PTS2). Defects in this gene cause peroxisome biogenesis disorders (PBDs), which are characterized by multiple defects in peroxisome function. There are at least 14 complementation groups for PBDs, with more than one phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene have been associated with PBD complementation group 11 (PBD-CG11) disorders, rhizomelic chondrodysplasia punctata type 1 (RCDP1), and Refsum disease (RD). provided by RefSeq, Oct 2008
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_000288" in other vectors (7)
SKU Description Size Price
RC202432 PEX7 (Myc-DDK-tagged)-Human peroxisomal biogenesis factor 7 (PEX7) 10 ug
$300.00
RC202432L1 Lenti ORF clone of Human peroxisomal biogenesis factor 7 (PEX7), Myc-DDK-tagged 10 ug
$600.00
RC202432L2 Lenti ORF clone of Human peroxisomal biogenesis factor 7 (PEX7), mGFP tagged 10 ug
$600.00
RC202432L3 Lenti ORF clone of Human peroxisomal biogenesis factor 7 (PEX7), Myc-DDK-tagged 10 ug
$600.00
RC202432L4 Lenti ORF clone of Human peroxisomal biogenesis factor 7 (PEX7), mGFP tagged 10 ug
$600.00
SC119985 PEX7 (untagged)-Human peroxisomal biogenesis factor 7 (PEX7) 10 ug
$300.00
SC324400 PEX7 (untagged)-Human peroxisomal biogenesis factor 7 (PEX7) 10 ug
$300.00

Plasmid Map for RG202432

Download sequence for

Cloning scheme for RG202432

Circular map for RG202432

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products