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PEX7 (NM_000288) Human Untagged Clone

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SKU
SC119985
PEX7 (untagged)-Human peroxisomal biogenesis factor 7 (PEX7)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PEX7
Synonyms PBD9B; PTS2R; RCDP1; RD
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_000288, the custom clone sequence may differ by one or more nucleotides


ATGAGTGCGGTGTGCGGTGGAGCGGCGCGGATGCTGCGGACGCCGGGACGCCACGGCTACGCCGCCGAGT
TCTCCCCGTACCTGCCGGGCCGCCTGGCCTGCGCCACCGCGCAGCACTACGGCATCGCGGGCTGTGGAAC
CCTACTAATATTGGATCCAGATGAAGCTGGGCTAAGGCTTTTTAGAAGCTTTGACTGGAATGATGGTTTG
TTTGATGTGACTTGGAGTGAGAACAACGAACATGTCCTCATCACCTGTAGTGGCGATGGCTCGCTGCAGC
TCTGGGACACTGCCAAAGCTGCAGGGCCACTGCAAGTCTATAAAGAACACGCTCAGGAGGTGTATAGTGT
TGATTGGAGCCAAACCAGAGGTGAACAGCTTGTGGTGTCTGGCTCATGGGATCAAACTGTCAAATTGTGG
GATCCAACTGTTGGAAAGTCTCTGTGCACCTTTAGAGGCCATGAAAGTATTATTTATAGCACAATCTGGT
CTCCCCACATCCCTGGTTGTTTTGCTTCAGCCTCAGGTGATCAGACTCTGAGAATATGGGATGTGAAGGC
AGCAGGAGTAAGAATCGTGATTCCTGCACATCAGGCAGAAATCTTGAGTTGTGACTGGTGTAAATACAAT
GAGAATTTGCTGGTGACCGGGGCGGTTGACTGTAGTTTGAGAGGCTGGGACTTAAGGAATGTACGACAAC
CAGTGTTTGAACTTCTTGGTCATACCTATGCTATTAGGAGGGTGAAATTTTCACCATTTCATGCTTCTGT
GCTGGCCTCTTGCTCGTATGATTTTACTGTAAGATTCTGGAACTTTTCAAAGCCTGACTCTCTTCTTGAA
ACAGTGGAGCATCATACAGAGTTTACTTGTGGTTTAGACTTCAGTCTTCAGAGCCCCACTCAGGTGGCTG
ACTGTTCTTGGGATGAAACAATAAAGATCTATGACCCTGCTTGTCTTACTATTCCTGCTTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_000288 unedited
AAAAAAGGTGCGNGATTAGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGCC
GACTCGGNACGGCTTCCGCGGCCGGGGCAGCGAGGGCCGGGGGCGGCGGGCGGGATGAGT
GCGGTGTGCGGTGGAGCGGCGCGGATGCTGCGGACGCCGGGACGCCACGGCTACGCCGCC
GAGTTCTCCCCGTACCTGCCGGGCCGCCTGGCCTGCGCCACCGCGCAGCACTACGGCATC
GCGGGCTGTGGAACCCTACTAATATTGGATCCAGATGAAGCTGGGCTAAGGCTTTTTAGA
AGCTTTGACTGGAATGATGGTTTGTTTGATGTGACTTGGAGTGAGAACAACGAACATGTC
CTCATCACCTGTAGTGGCGATGGCTCGCTGCAGCTCTGGGACACTGCCAAAGCTGCAGGG
CCACTGCAAGTCTATAAAGAACACGCTCAGGAGGTGTATAGTGTTGATTGGAGCCAAACC
AGAGGTGAACAGCTTGTGGTGTCTGGCTCATGGGATCAAACTGTCAAATTGTGGGATCCA
ACTGTTGGAAAGTCTCTGTGCACCTTTAGAGGCCATGAAAGTATTATTTATAGCACAATC
TGGTCTCCCCACATCCCTGGTTGTTTTGCTTCAGCCTCAGGTGATCAGACTCTGAGAATA
TGGGATGTGAAGGCAGCAGGAGTTAGAATCGGGATTCCTGCACCTNAGGCACAAATCCTG
AATTGTGACTGGTGTAAATACAATGAGTATTTGCCTGGTGACCGTGGCGGTTGACTGTAT
TTTGAGAGGCTGGCACTAAGGAATGTCCCAAACCCGTGGTTGAACTTTTTTGGTCTACCC
ATGCTTTAAGAGGGTGGAATTTTCACCATTCTAGCCTCTCGGCTGGCCCTTTGTTCGAAG
ATTTAACGCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_000288 unedited
ATGCCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGTT
TTAAATTACAAGTCTTTGTTTCCAAAAAACGTGATAAAACGTTTTGTTAATAAAAATCTC
TTGCCTTCTAAAAGGAAAGGAAATAATTTCATTTCATTGTAAATATTATCTGGGTTAGAT
ATTTTCTGAAACAAGGTCTGTCACCTAGGCTGGAATGCCTTAATCTGGGTCACTGCAGCC
TCAACTTCCCAGGATCAAGCAATCCTCCTGCCTTAGCCTCCTGAGTATCTGGGACTACAG
GCACACGTCACCATGCCTGACTACTTTGGGTTGGATACTTTTAGGGCCTATCACAAATTC
TGCTGATACAGAGAACACCCATTGTAGACCCACTTTTACTGACCACACCTTGGTGTAATG
CCATTAGTTCATATAGAGGCAAGCAGCAAGAAAAAAGGCTGTCAAACTCATCAGTGTGAC
AACTCTAAGGATGTGGTTAAGCACCATGTTCCCTGCAGTATACTTAGGTTAAGCAGTGTC
CTCTTTATGGGTCCTCTCAAAAGCAATCTGTATTGCTGATTCAAGAACCAAAAAAAAGTG
GGCACTGTGGCATGTCCCCATATCCCCACGTAATTGGGAGCCTGAGGGGGAAGGAGGGCT
TCAGCCTAGGATTCAAAACCCGTTTTGGGCAACATAGTGAGGCCCCATGCCTGACCCCCT
TTTGTTGCCTGTGCCCAGCAAGTGGGCAGGGCCCAACCCTGCCCCAAGGCCAAAACCTGC
CCCTCTGGCCCTAGCCCCTGACCTGCGAAACTGGTCACAATTCCTTTATCTTGAACCCCC
AAACACTCACCGGCAATTTTTGGCCCCACCCCTATAACCTTTCAGGACCTTGCGGGATTC
CTACACAACCCCCTTCTTTGCCC
Restriction Sites NotI-NotI |
ACCN NM_000288
Insert Size 3270 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000288.1, NP_000279.1
RefSeq Size 1451 bp
RefSeq ORF 972 bp
Locus ID 5191
UniProt ID O00628
Cytogenetics 6q23.3
Domains WD40
Protein Families Druggable Genome
Summary This gene encodes the cytosolic receptor for the set of peroxisomal matrix enzymes targeted to the organelle by the peroxisome targeting signal 2 (PTS2). Defects in this gene cause peroxisome biogenesis disorders (PBDs), which are characterized by multiple defects in peroxisome function. There are at least 14 complementation groups for PBDs, with more than one phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene have been associated with PBD complementation group 11 (PBD-CG11) disorders, rhizomelic chondrodysplasia punctata type 1 (RCDP1), and Refsum disease (RD). provided by RefSeq, Oct 2008
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Documents
Resources
"NM_000288" in other vectors (7)
SKU Description Size Price
RC202432 PEX7 (Myc-DDK-tagged)-Human peroxisomal biogenesis factor 7 (PEX7) 10 ug
$300.00
RC202432L1 Lenti ORF clone of Human peroxisomal biogenesis factor 7 (PEX7), Myc-DDK-tagged 10 ug
$600.00
RC202432L2 Lenti ORF clone of Human peroxisomal biogenesis factor 7 (PEX7), mGFP tagged 10 ug
$600.00
RC202432L3 Lenti ORF clone of Human peroxisomal biogenesis factor 7 (PEX7), Myc-DDK-tagged 10 ug
$600.00
RC202432L4 Lenti ORF clone of Human peroxisomal biogenesis factor 7 (PEX7), mGFP tagged 10 ug
$600.00
RG202432 PEX7 (tGFP-tagged) - Human peroxisomal biogenesis factor 7 (PEX7) 10 ug
$500.00
SC324400 PEX7 (untagged)-Human peroxisomal biogenesis factor 7 (PEX7) 10 ug
$300.00

file-alt Citations

A PEX7-centered perspective on the peroxisomal targeting signal type 2-mediated protein import pathway
Rodrigues, Alencastre, Francisco et al
Mol Cell Biol (2014) 34 (15), 2917-28 DOI: 10.1128/MCB.01727-13
AI Summary: In this study, OriGene products—specifically, the PEX7 (OriGene, RC217654) and PEX5 (OriGene, RC204326) expression plasmids—were used to investigate the mechanisms of PEX7-dependent peroxisomal protein import and the distinct export pathways of PEX7 and PEX5.
Showing 1-1 of 1 papers.
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