LIN37 (NM_019104) Human Tagged ORF Clone

  • TrueORF®
SKU
RG202212
LIN37 (tGFP-tagged) - Human lin-37 homolog (C. elegans) (LIN37)
  $500.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LIN37
Synonyms F25965; lin-37; ZK418.4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG202212 representing NM_019104
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCCTGTGAAGGTGAAAGTGGAGAAATCAGAGCTGGAGATGGCCAAAGCCCGGAACCAACTGGATG
CTGTCTTGCAGTGTCTGCTGGAGAAGAGTCACATGGACAGGGAGCGTCTGGATGAGGAAGCTGGGAAAAC
ACCCTCAGACACCCACAATAAGGACTGCTCCATCGCAGCCACTGGCAAAAGGCCATCTGCCCGCTTCCCC
CACCAGCGGAGGAAGAAGAGGAGGGAGATGGATGATGGGCTGGCTGAGGGAGGGCCGCAGCGATCCAACA
CATATGTGATCAAGCTGTTCGACCGGAGCGTGGACTTGGCCCAGTTCAGCGAGAACACGCCACTGTACCC
AATCTGCCGCGCCTGGATGCGCAACAGCCCCTCTGTGCGCGAGCGTGAATGCTCTCCCAGCTCACCCCTG
CCCCCGCTGCCTGAGGATGAGGAGGGCTCAGAGGTAACCAACAGCAAGAGTCGTGATGTGTACAAGCTGC
CGCCACCCACACCCCCGGGGCCACCCGGAGATGCCTGCAGATCCCGCATCCCATCTCCACTGCAGCCTGA
GATGCAGGGCACCCCTGACGATGAGCCCTCTGAGCCCGAGCCCTCACCCTCCACACTCATCTATCGCAAC
ATGCAGCGCTGGAAACGCATCCGCCAGAGGTGGAAGGAGGCCTCTCATCGGAACCAGCTTCGTTACTCAG
AAAGCATGAAGATCCTACGAGAGATGTACGAACGACAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG202212 representing NM_019104
Red=Cloning site Green=Tags(s)

MFPVKVKVEKSELEMAKARNQLDAVLQCLLEKSHMDRERLDEEAGKTPSDTHNKDCSIAATGKRPSARFP
HQRRKKRREMDDGLAEGGPQRSNTYVIKLFDRSVDLAQFSENTPLYPICRAWMRNSPSVRERECSPSSPL
PPLPEDEEGSEVTNSKSRDVYKLPPPTPPGPPGDACRSRIPSPLQPEMQGTPDDEPSEPEPSPSTLIYRN
MQRWKRIRQRWKEASHRNQLRYSESMKILREMYERQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019104
ORF Size 738 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019104.3
RefSeq Size 929 bp
RefSeq ORF 741 bp
Locus ID 55957
UniProt ID Q96GY3
Cytogenetics 19q13.12
Summary This gene encodes a protein expressed in the eye. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_019104" in other vectors (4)
SKU Description Size Price
RC202212 LIN37 (Myc-DDK-tagged)-Human lin-37 homolog (C. elegans) (LIN37) 10 ug
$300.00
RC202212L3 Lenti ORF clone of Human lin-37 homolog (C. elegans) (LIN37), Myc-DDK-tagged 10 ug
$600.00
RC202212L4 Lenti ORF clone of Human lin-37 homolog (C. elegans) (LIN37), mGFP tagged 10 ug
$600.00
SC321541 LIN37 (untagged)-Human lin-37 homolog (C. elegans) (LIN37) 10 ug
$300.00

Plasmid Map for RG202212

Download sequence for

Cloning scheme for RG202212

Circular map for RG202212

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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