CD9 (NM_001769) Human Tagged ORF Clone
$489.00
$500.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CD9 |
| Synonyms | BTCC-1; DRAP-27; MIC3; MRP-1; TSPAN-29; TSPAN29 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | |
| ACCN | NM_001769 |
| ORF Size | 684 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001769.4 |
| RefSeq Size | 1246 bp |
| RefSeq ORF | 687 bp |
| Locus ID | 928 |
| UniProt ID | P21926 |
| Cytogenetics | 12p13.31 |
| Domains | transmembrane4 |
| Protein Families | Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Hematopoietic cell lineage |
| Summary | This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. provided by RefSeq, Jan 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001769" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202000 | CD9 (Myc-DDK-tagged)-Human CD9 molecule (CD9) | 10 ug |
$300.00
|
|
| RC202000L1 | Lenti ORF clone of Human CD9 molecule (CD9), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC202000L2 | Lenti ORF clone of Human CD9 molecule (CD9), mGFP tagged | 10 ug |
$600.00
|
|
| RC202000L3 | Lenti ORF clone of Human CD9 molecule (CD9), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC202000L4 | Lenti ORF clone of Human CD9 molecule (CD9), mGFP tagged | 10 ug |
$600.00
|
|
| SC119043 | CD9 (untagged)-Human CD9 molecule (CD9) | 10 ug |
$300.00
|
|
| SC322329 | CD9 (untagged)-Human CD9 molecule (CD9) | 10 ug |
$300.00
|
|
Citations
ER stress disrupts insulin release in murine models of type 2 diabetes by impairing retromer action and constitutive secretion
Sue, Thai, Boslem et al
Cell Rep (2025) 44 (5), 115691 DOI: 10.1016/j.celrep.2025.115691
Sue, Thai, Boslem et al
Cell Rep (2025) 44 (5), 115691 DOI: 10.1016/j.celrep.2025.115691
Cited by 7
Ceramide-mediated orchestration of oxidative stress response through filopodia-derived small extracellular vesicles
Quadri, Elsherbini, Crivelli et al
J Extracell Vesicles (2024) 13 (7), e12477 DOI: 10.1002/jev2.12477
Quadri, Elsherbini, Crivelli et al
J Extracell Vesicles (2024) 13 (7), e12477 DOI: 10.1002/jev2.12477
Cited by 27
AI Summary: In this study, the OriGene CD9 (NM_001769) Human Tagged ORF Clone (tGFP-tagged, RG202000) was used to label and track small extracellular vesicles, enabling the authors to investigate the role of ceramide and sphingomyelinases in oxidative stress response and vesicle-mediated cell death.
Snippet: Hela (ATCC CCL‐2) N2a cells ATCC Neuro‐2a (ATCC CCL‐131) John Wiley & Sons, Ltd. PLASMID AND SIRNA SOURCE IDENTIFIER CD9 (NM_001769) Human Tagged ORF Clone (tGFP‐tagged) Origene RG202000 Homo sapiens sphingomyelin phosphodiesterase 1 (SMPD1), transcript variant 1 (ASM) Genecopoeia EX‐T8358‐M03 Mus musculus sphingomyelin phosphodiesterase 3, neutral (Sm
HIV-1-induced nuclear invaginations mediated by VAP-A, ORP3, and Rab7 complex explain infection of activated T cells
Santos, Rappa, Karbanová et al
Nat Commun (2023) 14 (1), 4588 DOI: 10.1038/s41467-023-40227-8
Santos, Rappa, Karbanová et al
Nat Commun (2023) 14 (1), 4588 DOI: 10.1038/s41467-023-40227-8
Cited by 17
AI Summary: OriGene's CD9-GFP plasmid (Cat# RG202000) was used in this study to express GFP-tagged CD9 in cells, enabling the investigation of HIV-1-induced nuclear envelope invaginations and the cellular mechanisms underlying viral nuclear entry.
Snippet: … Here, we report that in HeLa cells and Save;terminal GFP-tagged fusion protein versions of human VAP-A (GenBank Accession Number NM_194434.2;#HG11412-ACG;Sino Biological, Beijing, China) or CD9 (GenBank Accession Number NM_001769;#RG202000, OriGene Technologies, Rockville, MD) carried in the vectors pCMV3-C-GFPSpark (VAP-A-GFP plasmid) and pCMV6-AC-GFP (CD9-GFP plasmid), which contain the hygromycin and neomycin resistance genes, respect
Novel Isolation Method Reveals Sex-Specific Composition and Neurotoxicity of Small Extracellular Vesicles in a Mouse Model of Alzheimer's Disease
Elsherbini, Zhu, Quadri et al
Cells (2023) 12 (12) DOI: 10.3390/cells12121623
Elsherbini, Zhu, Quadri et al
Cells (2023) 12 (12) DOI: 10.3390/cells12121623
Cited by 12
AI Summary: OriGene’s CD9 turboGFP-tagged plasmid (OriGene-RG202000) was used to transfect astrocytes, enabling visualization and analysis of extracellular vesicle formation and protein interactions in the study.
Snippet: cells were passed to 24-well plates containing uncoated glass coverslips and cultured in DMEM (Life Technologies, Grand Island, NY, USA). Astrocytes were transfected with CD9 turboGFP-tagged plasmid (Origene-RG202000) using Lipofectamine 3000 Transfection Reagent (ThermoFisher- L3000008) according to the manufacturer’s protocol. The following day, cells were treated with 100 nM HiLyte Fluo
Anti-Human CD9 Fab Fragment Antibody Blocks the Extracellular Vesicle-Mediated Increase in Malignancy of Colon Cancer Cells
Santos, Rappa, Fontana et al
Cells (2022) 11 (16) DOI: 10.3390/cells11162474
Santos, Rappa, Fontana et al
Cells (2022) 11 (16) DOI: 10.3390/cells11162474
Cited by 24
AI Summary: OriGene’s pCMV6-AC-GFP plasmid (#RG202000), encoding CD9 with a C-terminal GFP tag, was used to transfect SW620 cells, enabling the study of CD9’s role in extracellular vesicle-mediated malignancy in colon cancer cells.
Snippet: … Plasmid and Transfection SW620 cells were transfected with pCMV6-AC-GFP plasmid encoding for CD9 with a GFP tag at its C-terminus under the control of the cytomegalovirus promoter (#RG202000;OriGene Technologies, Rockville, MD, USA) using Lipofectamine 3000 (#L3000008, Thermo Fisher Scientific) in a 1:2 DNA/lipid ratio. Cells expressing the neomycin resistance gene were selected by introd;SW620 cells were transfected with pCMV6-AC-GFP plasmid encoding for CD9 with a GFP tag at its C-terminus under the control of the cytomegalovirus promoter (#RG202000;OriGene Technologies, Rockville, MD, USA) using Lipofectamine 3000 (#L3000008, Thermo Fisher Scientific) in a 1:2 DNA/lipid ratio. …
Itraconazole inhibits nuclear delivery of extracellular vesicle cargo by disrupting the entry of late endosomes into the nucleoplasmic reticulum
Santos, Rappa, Karbanová et al
J Extracell Vesicles (2021) 10 (10), e12132 DOI: 10.1002/jev2.12132
Santos, Rappa, Karbanová et al
J Extracell Vesicles (2021) 10 (10), e12132 DOI: 10.1002/jev2.12132
Cited by 28
AI Summary: OriGene’s C-terminal GFP-tagged CD9 fusion protein (catalog #RG202000) was used in this study to visualize and track extracellular vesicle components, aiding in the investigation of how itraconazole disrupts nuclear delivery of EV cargo.
Snippet: C-terminal green fluorescent protein (GFP)-tagged fusion protein versions of CD9 (RG202000;OriGene Technologies, Rockville, MD, USA) or VAP-A (HG11412-ACG;Sino Biological, Beijing, China) were expressed in vectors pCMV6-AC-GFP or pCMV3-C-GFPSpark
Showing 1-6 of 10 papers.
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