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Lamin B Receptor (LBR) (NM_002296) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG201990
LBR (tGFP-tagged) - Human lamin B receptor (LBR), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$489.00 $772.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Lamin B Receptor
Synonyms C14SR; DHCR14B; LMN2R; PHA; PHASK; TDRD18
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201990 representing NM_002296
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAAGTAGGAAATTTGCCGATGGTGAAGTGGTAAGAGGTCGATGGCCTGGGAGTTCACTTTATTATG
AAGTAGAAATTCTGAGCCACGACAGCACCTCCCAGCTTTACACTGTGAAGTATAAAGATGGAACAGAGCT
TGAATTGAAAGAGAATGATATTAAGCCTTTAACTTCCTTTAGGCAAAGGAAAGGTGGCTCAACTTCCAGT
TCCCCTTCCAGACGCCGAGGGAGTCGATCAAGGTCACGCTCCCGATCCCCTGGTCGACCACCTAAAAGTG
CCCGCCGATCTGCTTCTGCTTCCCACCAGGCCGACATTAAGGAAGCAAGGAGGGAAGTGGAAGTTAAATT
GACTCCGCTGATTCTGAAGCCATTTGGAAATAGCATCAGCAGATATAATGGGGAGCCTGAGCATATTGAG
AGAAATGACGCACCTCATAAAAATACACAGGAAAAATTCAGTTTGTCACAAGAAAGCAGTTACATAGCAA
CACAGTATAGCCTTCGTCCAAGAAGAGAAGAAGTCAAATTAAAAGAAATAGATTCTAAGGAAGAAAAATA
CGTTGCAAAAGAACTGGCAGTGAGAACCTTTGAAGTGACCCCCATCCGGGCAAAGGACTTGGAGTTTGGA
GGAGTACCTGGTGTGTTTCTCATCATGTTTGGCCTGCCTGTGTTCCTCTTCCTGTTGCTGTTGATGTGTA
AACAGAAAGATCCCAGTCTTCTGAATTTCCCTCCTCCTTTGCCAGCTTTGTATGAGTTATGGGAAACCAG
AGTATTTGGGGTCTACCTCCTGTGGTTTTTGATTCAAGTCCTGTTCTACCTACTGCCAATTGGAAAGGTT
GTAGAAGGAACGCCTCTTATTGATGGAAGAAGACTCAAGTATAGATTAAATGGATTCTATGCTTTTATCC
TGACATCTGCAGTCATCGGAACATCTCTCTTCCAGGGCGTAGAGTTTCATTACGTGTACAGTCATTTTCT
TCAGTTTGCACTTGCGGCCACTGTTTTTTGTGTGGTCTTGAGTGTGTATCTCTACATGCGCTCTTTGAAA
GCGCCCCGGAATGACCTGTCGCCTGCCAGCTCTGGAAATGCTGTCTATGATTTCTTCATTGGCCGTGAAT
TAAACCCTCGAATTGGTACTTTTGATCTCAAATACTTTTGTGAATTGCGCCCCGGATTGATTGGATGGGT
GGTTATTAACTTGGTGATGCTTTTGGCTGAAATGAAAATACAGGACCGCGCTGTTCCATCCTTGGCCATG
ATTTTAGTTAATAGTTTCCAGCTTCTCTATGTGGTGGATGCTCTCTGGAATGAGGAAGCGTTGTTGACGA
CCATGGACATCATCCACGATGGATTTGGATTCATGCTGGCTTTTGGAGACTTGGTGTGGGTTCCCTTTAT
TTACAGCTTCCAAGCCTTTTATTTAGTCAGTCATCCAAATGAAGTGTCTTGGCCAATGGCTTCTCTAATT
ATTGTTCTGAAACTTTGTGGTTATGTAATCTTCCGAGGTGCAAATTCTCAGAAAAATGCATTCCGGAAAA
ATCCCAGTGATCCAAAGCTTGCACATTTAAAAACCATTCATACTTCAACGGGAAAAAATCTTCTAGTTTC
TGGATGGTGGGGCTTTGTTCGCCACCCCAATTACTTGGGTGATCTCATCATGGCCTTGGCGTGGTCCCTC
CCATGTGGTTTTAACCACATTCTGCCTTATTTCTACATAATTTATTTCACCATGTTGCTTGTCCACCGAG
AAGCTCGTGACGAGTACCACTGTAAGAAGAAATACGGCGTGGCTTGGGAAAAGTACTGTCAGCGTGTGCC
CTACCGTATATTTCCATACATCTAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201990 representing NM_002296
Red=Cloning site Green=Tags(s)

MPSRKFADGEVVRGRWPGSSLYYEVEILSHDSTSQLYTVKYKDGTELELKENDIKPLTSFRQRKGGSTSS
SPSRRRGSRSRSRSRSPGRPPKSARRSASASHQADIKEARREVEVKLTPLILKPFGNSISRYNGEPEHIE
RNDAPHKNTQEKFSLSQESSYIATQYSLRPRREEVKLKEIDSKEEKYVAKELAVRTFEVTPIRAKDLEFG
GVPGVFLIMFGLPVFLFLLLLMCKQKDPSLLNFPPPLPALYELWETRVFGVYLLWFLIQVLFYLLPIGKV
VEGTPLIDGRRLKYRLNGFYAFILTSAVIGTSLFQGVEFHYVYSHFLQFALAATVFCVVLSVYLYMRSLK
APRNDLSPASSGNAVYDFFIGRELNPRIGTFDLKYFCELRPGLIGWVVINLVMLLAEMKIQDRAVPSLAM
ILVNSFQLLYVVDALWNEEALLTTMDIIHDGFGFMLAFGDLVWVPFIYSFQAFYLVSHPNEVSWPMASLI
IVLKLCGYVIFRGANSQKNAFRKNPSDPKLAHLKTIHTSTGKNLLVSGWWGFVRHPNYLGDLIMALAWSL
PCGFNHILPYFYIIYFTMLLVHREARDEYHCKKKYGVAWEKYCQRVPYRIFPYIY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002296
ORF Size 1845 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002296.4
RefSeq Size 3806 bp
RefSeq ORF 1848 bp
Locus ID 3930
UniProt ID Q14739
Cytogenetics 1q42.12
Domains ERG4_ERG24, TUDOR
Protein Families Druggable Genome, Transmembrane
Summary The protein encoded by this gene belongs to the ERG4/ERG24 family. It localized in the nuclear envelope inner membrane and anchors the lamina and the heterochromatin to the membrane. It may mediate interaction between chromatin and lamin B. Mutations of this gene has been associated with autosomal recessive HEM/Greenberg skeletal dysplasia. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_002296" in other vectors (6)
SKU Description Size Price
RC201990 LBR (Myc-DDK-tagged)-Human lamin B receptor (LBR), transcript variant 1 10 ug
$572.00
RC201990L1 Lenti ORF clone of Human lamin B receptor (LBR), transcript variant 1, Myc-DDK-tagged 10 ug
$872.00
RC201990L2 Lenti ORF clone of Human lamin B receptor (LBR), transcript variant 1, mGFP tagged 10 ug
$872.00
RC201990L3 Lenti ORF clone of Human lamin B receptor (LBR), transcript variant 1, Myc-DDK-tagged 10 ug
$872.00
RC201990L4 Lenti ORF clone of Human lamin B receptor (LBR), transcript variant 1, mGFP tagged 10 ug
$872.00
SC109384 LBR (untagged)-Human lamin B receptor (LBR), transcript variant 1 10 ug
$573.00

Plasmid Map for RG201990

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG201990
Circular map for RG201990

file-alt Citations

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