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TRIM38 (NM_006355) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG201944
TRIM38 (tGFP-tagged) - Human tripartite motif containing 38 (TRIM38)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "TRIM38 or NM_006355"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TRIM38
Synonyms RNF15; RORET
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201944 representing NM_006355
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCAACCACCAGCACCAAGAAGATGATGGAGGAAGCCACCTGCTCCATCTGCCTGAGCCTGATCA
CGAACCCAGTAAGCATCAACTGTGGACACAGCTACTGCCACTTGTGTATAACAGACTTCTTTAAAAACCC
AAGCCAAAAGCAACTGAGGCAGGAGACATTCTGCTGTCCCCAGTGTCGGGCTCCATTTCATATGGATAGC
CTCCGACCCAACAAGCAGCTGGGAAGCCTCATTGAAGCCCTCAAAGAGACGGATCAAGAAATGTCATGTG
AGGAACACGGAGAGCAGTTCCACCTGTTCTGCGAAGACGAGGGGCAGCTCATCTGCTGGCGCTGTGAGCG
GGCACCACAGCACAAAGGGCACACCACAGCTCTTGTTGAAGACGTATGCCAGGGCTACAAGGAAAAGCTC
CAGAAAGCTGTGACAAAACTGAAGCAACTTGAAGACAGATGTACGGAGCAGAAGCTGTCCACAGCAATGC
GAATAACTAAATGGAAAGAGAAGGTACAGATTCAGAGACAAAAAATCCGGTCTGACTTTAAGAATCTCCA
GTGTTTCCTACATGAGGAAGAGAAGTCTTATCTCTGGAGGCTGGAGAAAGAAGAACAACAGACTCTGAGT
AGACTGAGGGACTATGAGGCTGGTCTGGGGCTGAAGAGCAATGAACTCAAGAGCCACATCCTGGAACTGG
AGGAAAAATGTCAGGGCTCAGCCCAGAAATTGCTGCAGAATGTGAATGACACTTTGAGCAGGAGTTGGGC
TGTGAAGCTGGAAACATCAGAGGCTGTCTCCTTGGAACTTCATACTATGTGCAATGTTTCCAAGCTTTAC
TTCGATGTGAAGAAAATGTTAAGGAGTCATCAAGTTAGTGTGACTCTGGATCCAGATACAGCTCATCACG
AACTAATTCTCTCTGAGGATCGGAGACAAGTGACTCGTGGATACACCCAGGAGAATCAGGACACATCTTC
CAGGAGATTTACTGCCTTCCCCTGTGTCTTGGGTTGTGAAGGCTTCACCTCAGGAAGACGTTACTTTGAA
GTGGATGTTGGCGAAGGAACCGGATGGGATTTAGGAGTTTGTATGGAAAATGTGCAGAGGGGCACTGGCA
TGAAGCAAGAGCCTCAGTCTGGATTCTGGACCCTCAGGCTGTGCAAAAAGAAAGGCTATGTAGCACTTAC
TTCTCCCCCAACTTCCCTTCATCTGCATGAGCAGCCCCTGCTTGTGGGAATTTTTCTGGACTATGAGGCC
GGAGTTGTATCCTTTTATAACGGGAATACTGGCTGCCACATCTTTACTTTCCCGAAGGCTTCCTTCTCTG
ATACTCTCCGGCCCTATTTCCAGGTTTATCAATATTCTCCTTTGTTTCTGCCTCCCCCAGGTGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201944 representing NM_006355
Red=Cloning site Green=Tags(s)

MASTTSTKKMMEEATCSICLSLITNPVSINCGHSYCHLCITDFFKNPSQKQLRQETFCCPQCRAPFHMDS
LRPNKQLGSLIEALKETDQEMSCEEHGEQFHLFCEDEGQLICWRCERAPQHKGHTTALVEDVCQGYKEKL
QKAVTKLKQLEDRCTEQKLSTAMRITKWKEKVQIQRQKIRSDFKNLQCFLHEEEKSYLWRLEKEEQQTLS
RLRDYEAGLGLKSNELKSHILELEEKCQGSAQKLLQNVNDTLSRSWAVKLETSEAVSLELHTMCNVSKLY
FDVKKMLRSHQVSVTLDPDTAHHELILSEDRRQVTRGYTQENQDTSSRRFTAFPCVLGCEGFTSGRRYFE
VDVGEGTGWDLGVCMENVQRGTGMKQEPQSGFWTLRLCKKKGYVALTSPPTSLHLHEQPLLVGIFLDYEA
GVVSFYNGNTGCHIFTFPKASFSDTLRPYFQVYQYSPLFLPPPGD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006355
ORF Size 1395 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006355.2, NP_006346.1
RefSeq Size 3286 bp
RefSeq ORF 1398 bp
Locus ID 10475
UniProt ID O00635
Cytogenetics 6p22.2
Domains PRY, RING, SPRY, zf-B_box
Protein Families Druggable Genome
Summary This gene encodes a member of the tripartite motif (TRIM) family. The encoded protein contains a RING-type zinc finger, B box-type zinc finger and SPRY domain. The function of this protein has not been identified. A pseudogene of this gene is located on the long arm of chromosome 4. provided by RefSeq, Jul 2012
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"NM_006355" in other vectors (6)
SKU Description Size Price
RC201944 TRIM38 (Myc-DDK-tagged)-Human tripartite motif containing 38 (TRIM38) 10 ug
$457.00
RC201944L1 Lenti ORF clone of Human tripartite motif containing 38 (TRIM38), Myc-DDK-tagged 10 ug
$757.00
RC201944L2 Lenti ORF clone of Human tripartite motif containing 38 (TRIM38), mGFP tagged 10 ug
$757.00
RC201944L3 Lenti ORF clone of Human tripartite motif containing 38 (TRIM38), Myc-DDK-tagged 10 ug
$757.00
RC201944L4 Lenti ORF clone of Human tripartite motif containing 38 (TRIM38), mGFP tagged 10 ug
$757.00
SC116143 TRIM38 (untagged)-Human tripartite motif containing 38 (TRIM38) 10 ug
$457.00

Plasmid Map for RG201944

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG201944
Circular map for RG201944

file-alt Citations

E3 ubiquitin ligase tripartite motif 38 negatively regulates TLR-mediated immune responses by proteasomal degradation of TNF receptor-associated factor 6 in macrophages
Zhao, Wang, Zhang et al
J. Immunol. (2012) 188 (6), 2567-74 DOI: 10.4049/jimmunol.1103255
Cited by 127
AI Summary: OriGene contributed to this study by providing the Flag-tagged TRIM38 (NM_006355) expression plasmid, which was used to investigate the regulatory role of TRIM38 in TLR-mediated immune responses.
Snippet: Their respective HRP-conjugated secondary Abs were purchased from Santa Cruz Biotechnology. Sequences, plasmid constructs, and transfection. Flag-tagged TRIM38 (NM_006355) expression plasmid was purchased from OriGene (Rockville, MD).
Showing 1-1 of 1 papers.
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