TIMP1 (NM_003254) Human Tagged ORF Clone
$650.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TIMP1 |
| Synonyms | CLGI; EPA; EPO; HCI; TIMP; TIMP-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | |
| ACCN | NM_003254 |
| ORF Size | 621 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003254.1 |
| RefSeq Size | 931 bp |
| RefSeq ORF | 624 bp |
| Locus ID | 7076 |
| UniProt ID | P01033 |
| Cytogenetics | Xp11.3 |
| Domains | NTR |
| Protein Families | Druggable Genome, Secreted Protein |
| Summary | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003254" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201548 | TIMP1 (Myc-DDK-tagged)-Human TIMP metallopeptidase inhibitor 1 (TIMP1) | 10 ug |
$450.00
|
|
| RC201548L1 | Lenti ORF clone of Human TIMP metallopeptidase inhibitor 1 (TIMP1), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC201548L2 | Lenti ORF clone of Human TIMP metallopeptidase inhibitor 1 (TIMP1), mGFP tagged | 10 ug |
$750.00
|
|
| RC201548L3 | Lenti ORF clone of Human TIMP metallopeptidase inhibitor 1 (TIMP1), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC201548L4 | Lenti ORF clone of Human TIMP metallopeptidase inhibitor 1 (TIMP1), mGFP tagged | 10 ug |
$750.00
|
|
| SC118082 | TIMP1 (untagged)-Human TIMP metallopeptidase inhibitor 1 (TIMP1) | 10 ug |
$300.00
|
|
Citations
Substrate Stiffness-Driven Membrane Tension Modulates Vesicular Trafficking via Caveolin-1
Lachowski, Matellan, Gopal et al
ACS Nano (2022) 16 (3), 4322-4337 DOI: 10.1021/acsnano.1c10534
Lachowski, Matellan, Gopal et al
ACS Nano (2022) 16 (3), 4322-4337 DOI: 10.1021/acsnano.1c10534
Cited by 76
AI Summary: OriGene products contributed to this study by providing the Caveolin-1 overexpression plasmid (Cat# RC217445) and TIMP-1 overexpression plasmid (Cat# RC210726), which were used to investigate how increased levels of these proteins affect vesicular trafficking and secretion in hepatic stellate cells under varying substrate stiffness conditions.
Snippet: in-1 Overexpression in Hepatic Stellate Cells Hepatic stellate cells (HSCs) are involved in wound healing in response to liver injury but can become dysregulated in HCC and promote desmoplasia and tumorigenesis.5,6 Given their central role in fibrosis, cancer development, and matrix remodeling, HSCs are an ideal model cell to investigate the upregulation of TIMP-1 and caveolin-1 in liver disease
Angiogenic factor-driven inflammation promotes extravasation of human proangiogenic monocytes to tumours
Sidibe, Ropraz, Jemelin et al
Nat Commun (2018) 9 (1), 355 DOI: 10.1038/s41467-017-02610-0
Sidibe, Ropraz, Jemelin et al
Nat Commun (2018) 9 (1), 355 DOI: 10.1038/s41467-017-02610-0
AI Summary: OriGene's TIMP1-GFP and empty-GFP vectors were used to generate stably transfected DLD1 cell lines, enabling the study of how TIMP1 overexpression affects the proangiogenic function of CD16+ monocytes in tumour models.
Snippet: the involvement of matrix metalloproteinases (MMPs) in proangiogenic function of CD16+ monocytes, DLD1 cells were stably transfected with TIMP1-GFP or empty-GFP vectors with neomycin resistance gene (Origene). … TIMP1 overexpression was confirmed by quantitative PCR analysis;To study the involvement of matrix metalloproteinases (MMPs) in proangiogenic function of CD16+ monocytes, DLD1 cells were stably transfected with TIMP1-GFP or empty-GFP vectors with neomycin resistance gene (Origene). …
Identification of a cytogenetic and molecular subgroup of acute myeloid leukemias showing sensitivity to L-Asparaginase
Bertuccio, Serravalle, Astolfi et al
Oncotarget (2017) 8 (66), 109915-109923 DOI: 10.18632/oncotarget.18565
Bertuccio, Serravalle, Astolfi et al
Oncotarget (2017) 8 (66), 109915-109923 DOI: 10.18632/oncotarget.18565
Snippet: … To study the involvement of matrix metalloproteinases (MMPs) in proangiogenic function of CD16+ monocytes, DLD1 cells were stably transfected with TIMP1-GFP or empty-GFP vectors with neomycin resistance gene (Origene). … TIMP1 overexpression was confirmed by quantitative PCR analysis. The effect of TIMP1 overexpression on DLD1
Down-regulation of TIMP-1 inhibits cell migration, invasion, and metastatic colonization in lung adenocarcinoma
Chang, Chiu, Cheng et al
Tumour Biol. (2015)
Chang, Chiu, Cheng et al
Tumour Biol. (2015)
Cited by 26
AI Summary: In this study, TIMP-1 plasmids (human gene BC000866) from OriGene were used to overexpress TIMP-1 in lung adenocarcinoma cells, enabling investigation of TIMP-1’s role in cell migration, invasion, and metastatic colonization.
Snippet: TIMP-1 overexpression in CL1-0 cells was achieved by transient transfection with TIMP-1 plas- mids (human gene BC000866, OriGene Technologies, Inc., Rockville, MD, USA) using TurboFect transfection reagent following the manufacturer's instructions (Thermo Fisher ... Related articles All 3 versions Cite SaveSaving
Showing 1-4 of 4 papers.
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