TIMP1 (NM_003254) Human Untagged Clone

SKU SC118082

TIMP1 (untagged)-Human TIMP metallopeptidase inhibitor 1 (TIMP1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TIMP1
• Synonyms: CLGI; EPA; EPO; HCI; TIMP; TIMP-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118082 sequence for NM_003254 edited (data generated by NextGen Sequencing); ATGGCCCCCTTTGAGCCCCTGGCTTCTGGCATCCTGTTGTTGCTGTGGCTGATAGCCCCC; AGCAGGGCCTGCACCTGTGTCCCACCCCACCCACAGACGGCCTTCTGCAATTCCGACCTC; GTCATCAGGGCCAAGTTCGTGGGGACACCAGAAGTCAACCAGACCACCTTATACCAGCGT; TATGAGATCAAGATGACCAAGATGTATAAAGGGTTCCAAGCCTTAGGGGATGCCGCTGAC; ATCCGGTTCGTCTACACCCCCGCCATGGAGAGTGTCTGCGGATACTTCCACAGGTCCCAC; AACCGCAGCGAGGAGTTTCTCATTGCTGGAAAACTGCAGGATGGACTCTTGCACATCACT; ACCTGCAGTTTCGTGGCTCCCTGGAACAGCCTGAGCTTAGCTCAGCGCCGGGGCTTCACC; AAGACCTACACTGTTGGCTGTGAGGAATGCACAGTGTTTCCCTGTTTATCCATCCCCTGC; AAACTGCAGAGTGGCACTCATTGCTTGTGGACGGACCAGCTCCTCCAAGGCTCTGAAAAG; GGCTTCCAGTCCCGTCACCTTGCCTGCCTGCCTCGGGAGCCAGGGCTGTGCACCTGGCAG; TCCCTGCGGTCCCAGATAGCCTGA; ; Clone variation with respect to NM_003254.2; 372 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003254 unedited; GGTAACATCTTGGATTTTGTAATCACGACTTACTATNAGGGCGGCACGCGCAATTCGGCA; CGAGGCCCAGCGCCCAGAANAAACACCAGAANAACCCACCATGGCCCCCTTTGAGCCCCT; GGCTTCTGGCATCCTGTTGTTGCTGTGGCTGATAGCCCCCAGCAGGGCCTGCACCTGTGT; CCCACCCCACCCACAGACGGCCTTCTGCAATTCCGACCTCGTCATCAGGGCCAAGTTCGT; GGGGACACCAGAAGTCAACCAGACCACCTTATACCAGCGTTATGAGATCAAGATGACCAA; GATGTATAAAGGGTTCCAAGCCTTAGGGGATGCCGCTGACATCCGGTTCGTCTACACCCC; CGCCATGGAGAGTGTCTGCGGATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCT; CATTGCTGGAAAACTGCAGGATGGACTCTTGCACATCACTACCTGCAGTTTCGTGGCTCC; CTGGAACAGCCTGAGCTTAGCTCAGCGCCGGGGCTTCACCAAGACCTACACTGTTGGCTG; TGAGGAATGCACAGTGTTTCCCTGTTTATCCATCCCCTGCAAACTGCAGAGTGGCACTCA; TTGCTTGTGGACGGACCAGCTCCTCCAAGGCTCTGAAAAGGGCTTCCAGTCCCGTCACCT; TGCCTGCCTGCCTCGGGAGCCAGGGCTGTGCACCTGGCAGTCCCTGCGGTCCCAGATAGC; CTGAATCCTGCCCGGAGTGGAAGCTGAAGCCTGCACAGTGTCCACCCTGTTCCCACTCCC; ATCTTTCTTNCCCGGACAATGAATANAGAGTTACCACCCAGCAAAAAAAAAAAAAAAAAA; CTCGACTCTAGATTGCGGCCCGCGTCATAGCTGTTTCCTGAACAGACCCNGGNTGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003254 unedited; CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTGCTGGGTGGTAACTCTTTATTTC; ATTGTCCGGAAGAAAGAGGGAGTGGGAACAGGGTGGACACTGTGCAGGCTTCAGCTTCCA; CTCCGGGCAGGATTCAGGCTATCTGGGACCGCAGGGACTGCCAGGTGCACAGCCCTGGCT; CCCGAGGCAGGCAGGCAAGGTGACGGGACTGGAAGCCCTTTTCAGAGCCTTGGAGGAGCT; GGTCCGTCCACAAGCAATGAGTGCCACTCTGCAGTTTGCAGGGGATGGATAAACAGGGAA; ACACTGTGCATTCCTCACAGCCAACAGTGTAGGTCTTGGTGAAGCCCCGGCGCTGAGCTA; AGCTCAGGCTGTTCCAGGGAGCCACGAAACTGCAGGTAGTGATGTGCAAGAGTCCATCCT; GCAGTTTTCCAGCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGC; AGACACTCTCCATGGCGGGGGTGTAGACCAACCGGATGTCANCGGCATCCCCTAAGGCTT; GGAACCCTTTATACATCTTGGCCATCTTGATCTCATAACGCTGGTATAAGGTGGTCTGGT; TGACTTCTGGTGTCCCCACAAACTTGGCCCTGATGACCAGGTCCGAACTGCCACAACGCC; GCCTGTGGGTGGCGCGGGACACAGGTGCAAGCCCTGCTGGGGGCTATCAACCCCCGCAAC; CCAAGATGCCAAACCCAGGGGCTCAAAGGGGCTCTGGGGGGTTCCCCTGTGCTTTCTTGG; ACGCTGGCTCCGGCCAACCCCGGGCCCGCCACANCCAGCCGATTCAAAATCCCGACGGTT; TCTAAACAAGTTCCGTTTATATAACTTCCCAGTACCCCCCCCCCCCATCGCCCCACGGGC; GCGGTCTTCGAATTCCGACAACACTGTTCCCTTGGCCCCACAAACCCCCTGGTCTTCAGG; GTCGCCCCTCTCAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_003254
• Insert Size: 624 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003254.2, NP_003245.1
• RefSeq Size: 931 bp
• RefSeq ORF: 624 bp
• Locus ID: 7076
• UniProt ID: P01033
• Cytogenetics: Xp11.3
• Domains: NTR
• Protein Families: Druggable Genome, Secreted Protein
• Summary: This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]