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TIMP1 (NM_003254) Human Untagged Clone

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SKU
SC118082
TIMP1 (untagged)-Human TIMP metallopeptidase inhibitor 1 (TIMP1)
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TIMP1
Synonyms CLGI; EPA; EPO; HCI; TIMP; TIMP-1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118082 sequence for NM_003254 edited (data generated by NextGen Sequencing)
ATGGCCCCCTTTGAGCCCCTGGCTTCTGGCATCCTGTTGTTGCTGTGGCTGATAGCCCCC
AGCAGGGCCTGCACCTGTGTCCCACCCCACCCACAGACGGCCTTCTGCAATTCCGACCTC
GTCATCAGGGCCAAGTTCGTGGGGACACCAGAAGTCAACCAGACCACCTTATACCAGCGT
TATGAGATCAAGATGACCAAGATGTATAAAGGGTTCCAAGCCTTAGGGGATGCCGCTGAC
ATCCGGTTCGTCTACACCCCCGCCATGGAGAGTGTCTGCGGATACTTCCACAGGTCCCAC
AACCGCAGCGAGGAGTTTCTCATTGCTGGAAAACTGCAGGATGGACTCTTGCACATCACT
ACCTGCAGTTTCGTGGCTCCCTGGAACAGCCTGAGCTTAGCTCAGCGCCGGGGCTTCACC
AAGACCTACACTGTTGGCTGTGAGGAATGCACAGTGTTTCCCTGTTTATCCATCCCCTGC
AAACTGCAGAGTGGCACTCATTGCTTGTGGACGGACCAGCTCCTCCAAGGCTCTGAAAAG
GGCTTCCAGTCCCGTCACCTTGCCTGCCTGCCTCGGGAGCCAGGGCTGTGCACCTGGCAG
TCCCTGCGGTCCCAGATAGCCTGA

Clone variation with respect to NM_003254.2
372 t=>c
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003254 unedited
GGTAACATCTTGGATTTTGTAATCACGACTTACTATNAGGGCGGCACGCGCAATTCGGCA
CGAGGCCCAGCGCCCAGAANAAACACCAGAANAACCCACCATGGCCCCCTTTGAGCCCCT
GGCTTCTGGCATCCTGTTGTTGCTGTGGCTGATAGCCCCCAGCAGGGCCTGCACCTGTGT
CCCACCCCACCCACAGACGGCCTTCTGCAATTCCGACCTCGTCATCAGGGCCAAGTTCGT
GGGGACACCAGAAGTCAACCAGACCACCTTATACCAGCGTTATGAGATCAAGATGACCAA
GATGTATAAAGGGTTCCAAGCCTTAGGGGATGCCGCTGACATCCGGTTCGTCTACACCCC
CGCCATGGAGAGTGTCTGCGGATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCT
CATTGCTGGAAAACTGCAGGATGGACTCTTGCACATCACTACCTGCAGTTTCGTGGCTCC
CTGGAACAGCCTGAGCTTAGCTCAGCGCCGGGGCTTCACCAAGACCTACACTGTTGGCTG
TGAGGAATGCACAGTGTTTCCCTGTTTATCCATCCCCTGCAAACTGCAGAGTGGCACTCA
TTGCTTGTGGACGGACCAGCTCCTCCAAGGCTCTGAAAAGGGCTTCCAGTCCCGTCACCT
TGCCTGCCTGCCTCGGGAGCCAGGGCTGTGCACCTGGCAGTCCCTGCGGTCCCAGATAGC
CTGAATCCTGCCCGGAGTGGAAGCTGAAGCCTGCACAGTGTCCACCCTGTTCCCACTCCC
ATCTTTCTTNCCCGGACAATGAATANAGAGTTACCACCCAGCAAAAAAAAAAAAAAAAAA
CTCGACTCTAGATTGCGGCCCGCGTCATAGCTGTTTCCTGAACAGACCCNGGNTGA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003254 unedited
CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTGCTGGGTGGTAACTCTTTATTTC
ATTGTCCGGAAGAAAGAGGGAGTGGGAACAGGGTGGACACTGTGCAGGCTTCAGCTTCCA
CTCCGGGCAGGATTCAGGCTATCTGGGACCGCAGGGACTGCCAGGTGCACAGCCCTGGCT
CCCGAGGCAGGCAGGCAAGGTGACGGGACTGGAAGCCCTTTTCAGAGCCTTGGAGGAGCT
GGTCCGTCCACAAGCAATGAGTGCCACTCTGCAGTTTGCAGGGGATGGATAAACAGGGAA
ACACTGTGCATTCCTCACAGCCAACAGTGTAGGTCTTGGTGAAGCCCCGGCGCTGAGCTA
AGCTCAGGCTGTTCCAGGGAGCCACGAAACTGCAGGTAGTGATGTGCAAGAGTCCATCCT
GCAGTTTTCCAGCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGC
AGACACTCTCCATGGCGGGGGTGTAGACCAACCGGATGTCANCGGCATCCCCTAAGGCTT
GGAACCCTTTATACATCTTGGCCATCTTGATCTCATAACGCTGGTATAAGGTGGTCTGGT
TGACTTCTGGTGTCCCCACAAACTTGGCCCTGATGACCAGGTCCGAACTGCCACAACGCC
GCCTGTGGGTGGCGCGGGACACAGGTGCAAGCCCTGCTGGGGGCTATCAACCCCCGCAAC
CCAAGATGCCAAACCCAGGGGCTCAAAGGGGCTCTGGGGGGTTCCCCTGTGCTTTCTTGG
ACGCTGGCTCCGGCCAACCCCGGGCCCGCCACANCCAGCCGATTCAAAATCCCGACGGTT
TCTAAACAAGTTCCGTTTATATAACTTCCCAGTACCCCCCCCCCCCATCGCCCCACGGGC
GCGGTCTTCGAATTCCGACAACACTGTTCCCTTGGCCCCACAAACCCCCTGGTCTTCAGG
GTCGCCCCTCTCAT
Restriction Sites NotI-NotI |
ACCN NM_003254
Insert Size 624 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003254.2, NP_003245.1
RefSeq Size 931 bp
RefSeq ORF 624 bp
Locus ID 7076
UniProt ID P01033
Cytogenetics Xp11.3
Domains NTR
Protein Families Druggable Genome, Secreted Protein
Summary This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_003254" in other vectors (6)
SKU Description Size Price
RC201548 TIMP1 (Myc-DDK-tagged)-Human TIMP metallopeptidase inhibitor 1 (TIMP1) 10 ug
$450.00
RC201548L1 Lenti ORF clone of Human TIMP metallopeptidase inhibitor 1 (TIMP1), Myc-DDK-tagged 10 ug
$750.00
RC201548L2 Lenti ORF clone of Human TIMP metallopeptidase inhibitor 1 (TIMP1), mGFP tagged 10 ug
$750.00
RC201548L3 Lenti ORF clone of Human TIMP metallopeptidase inhibitor 1 (TIMP1), Myc-DDK-tagged 10 ug
$750.00
RC201548L4 Lenti ORF clone of Human TIMP metallopeptidase inhibitor 1 (TIMP1), mGFP tagged 10 ug
$750.00
RG201548 TIMP1 (tGFP-tagged) - Human TIMP metallopeptidase inhibitor 1 (TIMP1) 10 ug
$650.00

file-alt Citations

Functionally enhanced cell spheroids for stem cell therapy: Role of TIMP1 in the survival and therapeutic effectiveness of stem cell spheroids
Choi, Chung, Oh et al
Acta Biomater (2023) 166, 454-469 DOI: 10.1016/j.actbio.2023.05.033
Cited by 19
AI Summary: In this study, OriGene's pCMV6-XL5 vector containing TIMP1 cDNA (SC118082) and the pCMV6-XL5 control vector were used to genetically overexpress or control TIMP1 levels in stem cell spheroids, enabling the researchers to demonstrate TIMP1’s key role in enhancing cell survival and therapeutic efficacy.
Snippet: … We developed cell Save;hASCs were transfected with a pCMV6-XL5 vector containing TIMP1 cDNA (SC118082;OriGene Technologies, Rockville, MD, USA) or pCMV6-XL5 control vector (PCMV6XL5;OriGene) using Lipofectamine 3000 (Thermo Fisher Scientific), according to the
Intrinsic dynamics study identifies two amino acids of TIMP-1 critical for its LRP-1-mediated endocytosis in neurons
Verzeaux, Belloy, Thevenard-Devy et al
Sci Rep (2017) 7 (1), 5375 DOI: 10.1038/s41598-017-05039-z
AI Summary: OriGene’s pCMV6-XL5 vector containing TIMP-1 cDNA was used as the template to generate TIMP-1 mutants, enabling the study of how specific amino acids affect TIMP-1’s endocytosis in neurons.
Snippet: The pCMV6-XL5 vector containing TIMP-1 cDNA was obtained from Origene (Origene, Rockville, USA). Mutated TIMP-1 forms were generated at the F12 and K47 positions using the QuickChange II Site-directed Mutagenesis kit (Stratagene, Agilent Technologies, Les Ulis, France) with the following primers
FigureFigureFigure
Posttranslational regulation of tissue inhibitor of metalloproteinase-7 by calcium-dependent vesicular exocytosis
Dranoff, JA;Bhatia, N;Fausther et al
Physiological Reports (2013)
AI Summary: OriGene contributed to this study by providing the TIMP-7 expression plasmid (Cat# RC217654) and anti-TIMP-7 antibody (Cat# TA326086), which were used to investigate the posttranslational regulation and detection of TIMP-7 in calcium-dependent vesicular exocytosis experiments.
Hyaluronan synthase 2 (HAS2) promotes breast cancer cell invasion by suppression of tissue metalloproteinase inhibitor 1 (TIMP-1)
Bernert, Porsch, Heldin
J. Biol. Chem. (2011) 286 (49), 42349-59 DOI: 10.1074/jbc.M111.278598
Cited by 11
AI Summary: OriGene’s pCMV6-XL5-TIMP-1 plasmid was used to overexpress TIMP-1 in breast cancer cells, helping researchers demonstrate that restoring TIMP-1 levels could rescue the loss of invasive capability caused by HAS2 knockdown.
Snippet: cells were incubated for 24 h. In some of the experiments, Matrigel-embedded MDA-MB-231- BM cells with HAS2 knocked down or not ... using SiLentFect transfection reagent (Bio-Rad) or enhanced by transfection with 0.5 μg of pCMV6-XL5-TIMP-1 (OriGene) using Lipofectamine
Showing 1-4 of 4 papers.
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