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PRSS56 (NM_001195129) Human Tagged ORF Clone

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SKU
RC231333
PRSS56 (Myc-DDK-tagged)-Human protease, serine, 56 (PRSS56)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $561.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PRSS56
Synonyms MCOP6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC231333 representing NM_001195129
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTGGCTGTGCTGCTGCTGCTACCCCTCCCAAGCTCATGGTTTGCCCACGGGCACCCACTGTACA
CACGCCTGCCCCCCAGCGCCCTGCAAGTTCTGTCGGCCCAGGGGACTCAGGCGTTGCAGGCAGCCCAGAG
GAGCGCCCAGTGGGCAATAAACCGAGTGGCGATGGAGATCCAGCACAGATCGCACGAGTGCCGAGGATCT
GGGCGCCCCAGGCCTCAAGCTCTCCTCCAGGACCCACCTGAGCCAGGGCCGTGCGGCGAGAGGCGTCCGA
GCACTGCCAATGTGACGCGGGCCCACGGCCGCATCGTGGGGGGCAGCGCGGCGCCGCCCGGGGCCTGGCC
CTGGCTGGTGAGGCTGCAGCTCGGCGGGCAGCCTCTGTGCGGCGGCGTCCTGGTAGCGGCCTCCTGGGTG
CTCACGGCAGCGCACTGCTTTGTAGGCGCCCCGAATGAGCTTCTGTGGACTGTGACGCTGGCAGAGGGGT
CCCGGGGGGAGCAAGCGGAGGAGGTGCCAGTGAACCGCATCCTGCCCCACCCCAAGTTTGACCCGCGGAC
CTTCCACAACGACCTGGCCCTGGTGCAGCTGTGGACGCCGGTGAGCCCGGGGGGATCGGCGCGCCCCGTG
TGCCTGCCCCAGGAGCCCCAGGAGCCCCCTGCCGGAACCGCCTGCGCCATCGCGGGCTGGGGCGCCCTCT
TCGAAGACGGGCCTGAGGCTGAAGCAGTGAGAGAGGCCCGTGTTCCCCTGCTCAGCACCGACACCTGCCG
AAGAGCCCTGGGGCCCGGGCTGCGCCCCAGCACCATGCTCTGCGCCGGGTACCTGGCGGGGGGCGTTGAC
TCGTGCCAGGGTGACTCGGGAGGCCCCCTGACCTGTTCTGAGCCTGGCCCCCGCCCTAGAGAGGTCCTGT
TCGGAGTCACCTCCTGGGGGGACGGCTGCGGGGAGCCAGGGAAGCCCGGGGTCTACACCCGCGTGGCAGT
GTTCAAGGACTGGCTCCAGGAGCAGATGAGCGCCTCCTCCAGCCGCGAGCCCAGCTGCAGGGAGCTTCTG
GCCTGGGACCCCCCCCAGGAGCTGCAGGCAGACGCCGCCCGGCTCTGCGCCTTCTATGCCCGCCTGTGCC
CGGGGTCCCAGGGCGCCTGTGCGCGCCTGGCGCACCAGCAGTGCCTGCAGCGCCGGCGGCGATGCGAGCT
GCGCTCGCTGGCGCACACGCTGCTGGGCCTGCTGCGGAACGCGCAGGAGCTGCTCGGGCCTCGTCCGGGA
CTGCGGCGCCTGGCCCCCGCCCTGGCTCTCCCCGCTCCAGCGCTCAGGGAGTCTCCTCTGCACCCCGCCC
GGGAGCTGCGGCTTCACTCAGGATCGCGGGCTGCAGGCACTCGGTTCCCGAAGCGGAGGCCGGAGCCGCG
CGGAGAAGCCAACGGCTGCCCTGGGCTGGAGCCCCTGCGACAGAAGTTGGCTGCCCTGCAGGGGGCCCAT
GCCTGGATCCTGCAGGTCCCCTCGGAGCACCTGGCCATGAACTTTCATGAGGTCCTGGCAGATCTGGGCT
CCAAGACACTGACCGGGCTTTTCAGAGCCTGGGTGCGGGCAGGCTTGGGGGGCCGGCATGTGGCCTTCAG
CGGCCTGGTGGGCCTGGAGCCGGCCACACTGGCTCGCAGCCTCCCCCGGCTGCTGGTGCAGGCCCTGCAG
GCCTTCCGCGTGGCTGCCCTGGCAGAAGGGGAGCCCGAGGGACCCTGGATGGATGTAGGGCAGGGGCCCG
GGCTGGAGAGGAAGGGGCACCACCCACTCAACCCTCAGGTACCCCCCGCCAGGCAACCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC231333 representing NM_001195129
Red=Cloning site Green=Tags(s)

MLLAVLLLLPLPSSWFAHGHPLYTRLPPSALQVLSAQGTQALQAAQRSAQWAINRVAMEIQHRSHECRGS
GRPRPQALLQDPPEPGPCGERRPSTANVTRAHGRIVGGSAAPPGAWPWLVRLQLGGQPLCGGVLVAASWV
LTAAHCFVGAPNELLWTVTLAEGSRGEQAEEVPVNRILPHPKFDPRTFHNDLALVQLWTPVSPGGSARPV
CLPQEPQEPPAGTACAIAGWGALFEDGPEAEAVREARVPLLSTDTCRRALGPGLRPSTMLCAGYLAGGVD
SCQGDSGGPLTCSEPGPRPREVLFGVTSWGDGCGEPGKPGVYTRVAVFKDWLQEQMSASSSREPSCRELL
AWDPPQELQADAARLCAFYARLCPGSQGACARLAHQQCLQRRRRCELRSLAHTLLGLLRNAQELLGPRPG
LRRLAPALALPAPALRESPLHPARELRLHSGSRAAGTRFPKRRPEPRGEANGCPGLEPLRQKLAALQGAH
AWILQVPSEHLAMNFHEVLADLGSKTLTGLFRAWVRAGLGGRHVAFSGLVGLEPATLARSLPRLLVQALQ
AFRVAALAEGEPEGPWMDVGQGPGLERKGHHPLNPQVPPARQP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001195129
ORF Size 1809 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001195129.2
RefSeq ORF 1812 bp
Locus ID 646960
UniProt ID P0CW18
Cytogenetics 2q37.1
MW 65 kDa
Summary This gene encodes a protein that contains a peptidase S1 domain and possesses trypsin-like serine protease activity. The encoded protein may play a role in eye development, and mutations in this gene are a cause of autosomal recessive posterior microphthalmos. provided by RefSeq, Dec 2011
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Documents
Resources
"NM_001195129" in other vectors (3)
SKU Description Size Price
RC231333L3 Lenti ORF clone of Human protease, serine, 56 (PRSS56), Myc-DDK-tagged 10 ug
$861.00
RC231333L4 Lenti ORF clone of Human protease, serine, 56 (PRSS56), mGFP tagged 10 ug
$861.00
RG231333 PRSS56 (tGFP-tagged) - Human protease, serine, 56 (PRSS56) 10 ug
$761.00

Plasmid Map for RC231333

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC231333
Circular map for RC231333

file-alt Citations

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