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MCF2 (NM_001171878) Human Tagged ORF Clone

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SKU
RC230549
MCF2 (Myc-DDK-tagged)-Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 5
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $787.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MCF2
Synonyms ARHGEF21; DBL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC230549 representing NM_001171878
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAAGCAAATCCCCGGAGAGGCAAGATGAGGTTCAGAAGGAATGCGGCTTCCTTCCCTGGGAACT
TGCACTTGGTTTTGGTTTTACGTCCTACCAGCTTTCTTCAACGAACGTTCACAGACATTGGATTTTGGTT
TAGTCAGGAGGATTTTATGCTTAAATTACCAGTTGTTATGCTGAGCTCAGTTAGTGATTTGCTGACATAC
ATTGATGACAAGCAATTAACCCCTGAGTTAGGCGGCACCTTGCAGTACTGCCACAGTGAATGGATCATCT
TCAGAAATGCTATAGAAAATTTTGCCCTCACAGTGAAAGAAATGGCTCAGATGTTACAGTCCTTTGGAAC
TGAACTGGCTGAGACAGAACTACCAGATGATATTCCCTCAATAGAAGAAATTCTGGCAATTCGTGCTGAA
AGGTATCATCTGTTGAAGAATGATATTACAGCTGTAACCAAAGAAGGAAAAATTCTGCTAACAAATCTGG
AAGTGCCTGACACTGAAGGAGCTGTCAGTTCAAGACTAGAATGTCATCGGCAAATAAGTGGTGACTGGCA
AACTATTAATAAGTTGCTGACTCAAGTACATGATATGGAAACAGCTTTTGATGGATTTTGGGAAAAACAT
CAATTAAAAATGGAGCAGTATCTGCAACTATGGAAGTTTGAGCAGGATTTTCAACAGCTTGTGACTGAAG
TTGAATTTCTATTAAACCAACAAGCAGAACTGGCTGATGTAACAGGGACTATAGCTCAAGTAAAACAAAA
AATAAAAAAATTGGAAAACTTAGATGAAAATTCTCAGGAGCTATTATCAAAGGCCCAGTTTGTGATATTA
CATGGACACAAGCTTGCAGCAAATCACCATTATGCACTTGATTTAATCTGCCAGAGGTGCAATGAGCTAC
GTTACCTTTCTGATATTTTGGTTAATGAGATAAAAGCAAAACGGATACAACTCAGCAGGACCTTCAAAAT
GCATAAACTCCTACAGCAGGCTCGTCAATGCTGTGATGAAGGGGAATGTCTTCTAGCTAATCAGGAAATA
GATAAGTTTCAGTCTAAAGAAGATGCTCAGAAAGCTCTCCAAGACATTGAAAATTTTCTTGAAATGGCTC
TACCCTTTATAAATTATGAACCTGAAACACTGCAGTATGAATTTGATGTAATATTATCTCCTGAGCTTAA
GGTTCAAATGAAGACTATACAACTCAAGCTTGAAAACATTCGAAGTATATTTGAGAACCAGCAGGCTGGT
TTCAGGAACCTGGCAGATAAGCATGTGAGGCCAATCCAATTTGTGGTACCCACACCTGAAAATTTGGTCA
CATCTGGGACACCATTTTTTTCATCTAAACAAGGGAAGAAGACTTGGAGACAAAATCAGAGCAACTTAAA
AATTGAAGTGGTGCCTGATTGTCAGGAGAAGAGAAGTTCTGGTCCATCCTCCAGTTTGGACAATGGCAAT
AGCTTGGATGTTTTAAAGAACCACGTACTAAATGAACTGATACAGACTGAGAGAGTTTATGTTCGAGAAC
TGTATACTGTTTTGTTGGGTTATAGAGCGGAGATGGATAATCCAGAGATGTTTGATCTTATGCCACCTCT
CCTGAGAAATAAAAAGGACATTCTCTTTGGAAACATGGCAGAAATATATGAATTCCATAACGACATTTTC
TTGAGCAGCCTGGAAAATTGTGCTCATGCTCCAGAAAGAGTGGGACCTTGTTTCCTGGAAAGGAAGGATG
ATTTTCAGATGTATGCAAAATATTGTCAGAATAAGCCCAGATCAGAAACAATTTGGAGGAAGTATTCAGA
ATGCGCATTTTTCCAGGAATGTCAAAGAAAGTTAAAACACAGACTTAGACTGGATTCCTATTTACTCAAA
CCAGTGCAACGAATCACTAAATATCAGTTATTGTTGAAGGAGCTATTAAAATATAGCAAAGACTGTGAAG
GATCTGCTCTGTTGAAGAAGGCACTCGATGCAATGCTGGATTTACTGAAGTCAGTTAATGATTCTATGCA
TCAGATTGCAATAAATGGCTATATTGGAAACTTAAATGAACTGGGCAAGATGATAATGCAAGGTGGATTC
AGCGTTTGGATAGGGCACAAGAAAGGTGCTACAAAAATGAAGGATTTGGCTAGATTCAAACCAATGCAGC
GACACCTTTTCTTGTATGAAAAAGCCATTGTTTTTTGCAAAAGGCGTGTTGAAAGTGGAGAAGGCTCTGA
CAGATACCCGTCATACAGTTTTAAACACTGTTGGAAAATGGATGAAGTTGGAATCACTGAATATGTAAAA
GGTGATAACCGCAAGTTTGAAATCTGGTATGGTGAAAAGGAAGAAGTTTATATTGTCCAGGCTTCTAATG
TAGATGTGAAGATGACGTGGCTAAAAGAAATAAGAAATATTTTGTTGAAGCAGCAGGAACTTTTGACAGT
TAAAAAAAGAAAGCAACAGGATCAATTAACAGAACGGGATAAGTTTCAGATTTCTCTTCAGCAGAATGAT
GAAGACCTGTGTCGGAGATGGCTCTCCTATATTGATGAAGCTACTATGTCAAATGGCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC230549 representing NM_001171878
Red=Cloning site Green=Tags(s)

MAEANPRRGKMRFRRNAASFPGNLHLVLVLRPTSFLQRTFTDIGFWFSQEDFMLKLPVVMLSSVSDLLTY
IDDKQLTPELGGTLQYCHSEWIIFRNAIENFALTVKEMAQMLQSFGTELAETELPDDIPSIEEILAIRAE
RYHLLKNDITAVTKEGKILLTNLEVPDTEGAVSSRLECHRQISGDWQTINKLLTQVHDMETAFDGFWEKH
QLKMEQYLQLWKFEQDFQQLVTEVEFLLNQQAELADVTGTIAQVKQKIKKLENLDENSQELLSKAQFVIL
HGHKLAANHHYALDLICQRCNELRYLSDILVNEIKAKRIQLSRTFKMHKLLQQARQCCDEGECLLANQEI
DKFQSKEDAQKALQDIENFLEMALPFINYEPETLQYEFDVILSPELKVQMKTIQLKLENIRSIFENQQAG
FRNLADKHVRPIQFVVPTPENLVTSGTPFFSSKQGKKTWRQNQSNLKIEVVPDCQEKRSSGPSSSLDNGN
SLDVLKNHVLNELIQTERVYVRELYTVLLGYRAEMDNPEMFDLMPPLLRNKKDILFGNMAEIYEFHNDIF
LSSLENCAHAPERVGPCFLERKDDFQMYAKYCQNKPRSETIWRKYSECAFFQECQRKLKHRLRLDSYLLK
PVQRITKYQLLLKELLKYSKDCEGSALLKKALDAMLDLLKSVNDSMHQIAINGYIGNLNELGKMIMQGGF
SVWIGHKKGATKMKDLARFKPMQRHLFLYEKAIVFCKRRVESGEGSDRYPSYSFKHCWKMDEVGITEYVK
GDNRKFEIWYGEKEEVYIVQASNVDVKMTWLKEIRNILLKQQELLTVKKRKQQDQLTERDKFQISLQQND
EDLCRRWLSYIDEATMSNGK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001171878
ORF Size 2580 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001171878.1, NP_001165349.1
RefSeq ORF 2583 bp
Locus ID 4168
UniProt ID P10911, B2R9S6
Cytogenetics Xq27.1
Protein Families Druggable Genome
MW 100.9 kDa
Summary The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.provided by RefSeq, Jan 2010
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Documents
Resources
"NM_001171878" in other vectors (6)
SKU Description Size Price
RC230549L1 Lenti ORF clone of Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 5, Myc-DDK-tagged 10 ug
$1,087.00
RC230549L2 Lenti ORF clone of Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 5, mGFP tagged 10 ug
$1,087.00
RC230549L3 Lenti ORF clone of Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 5, Myc-DDK-tagged 10 ug
$1,087.00
RC230549L4 Lenti ORF clone of Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 5, mGFP tagged 10 ug
$1,087.00
RG230549 MCF2 (tGFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 5 10 ug
$987.00
SC329187 MCF2 (untagged)-Human MCF.2 cell line derived transforming sequence (MCF2) transcript variant 5 10 ug
$867.00

Plasmid Map for RC230549

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC230549
Circular map for RC230549

file-alt Citations

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