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MCF2 (NM_001171878) Human Untagged Clone

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SKU
SC329187
MCF2 (untagged)-Human MCF.2 cell line derived transforming sequence (MCF2) transcript variant 5
  $867.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MCF2
Synonyms ARHGEF21; DBL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC329187 representing NM_001171878.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCAGAAGCAAATCCCCGGAGAGGCAAGATGAGGTTCAGAAGGAATGCGGCTTCCTTCCCTGGGAAC
TTGCACTTGGTTTTGGTTTTACGTCCTACCAGCTTTCTTCAACGAACGTTCACAGACATTGGATTTTGG
TTTAGTCAGGAGGATTTTATGCTTAAATTACCAGTTGTTATGCTGAGCTCAGTTAGTGATTTGCTGACA
TACATTGATGACAAGCAATTAACCCCTGAGTTAGGCGGCACCTTGCAGTACTGCCACAGTGAATGGATC
ATCTTCAGAAATGCTATAGAAAATTTTGCCCTCACAGTGAAAGAAATGGCTCAGATGTTACAGTCCTTT
GGAACTGAACTGGCTGAGACAGAACTACCAGATGATATTCCCTCAATAGAAGAAATTCTGGCAATTCGT
GCTGAAAGGTATCATCTGTTGAAGAATGATATTACAGCTGTAACCAAAGAAGGAAAAATTCTGCTAACA
AATCTGGAAGTGCCTGACACTGAAGGAGCTGTCAGTTCAAGACTAGAATGTCATCGGCAAATAAGTGGT
GACTGGCAAACTATTAATAAGTTGCTGACTCAAGTACATGATATGGAAACAGCTTTTGATGGATTTTGG
GAAAAACATCAATTAAAAATGGAGCAGTATCTGCAACTATGGAAGTTTGAGCAGGATTTTCAACAGCTT
GTGACTGAAGTTGAATTTCTATTAAACCAACAAGCAGAACTGGCTGATGTAACAGGGACTATAGCTCAA
GTAAAACAAAAAATAAAAAAATTGGAAAACTTAGATGAAAATTCTCAGGAGCTATTATCAAAGGCCCAG
TTTGTGATATTACATGGACACAAGCTTGCAGCAAATCACCATTATGCACTTGATTTAATCTGCCAGAGG
TGCAATGAGCTACGTTACCTTTCTGATATTTTGGTTAATGAGATAAAAGCAAAACGGATACAACTCAGC
AGGACCTTCAAAATGCATAAACTCCTACAGCAGGCTCGTCAATGCTGTGATGAAGGGGAATGTCTTCTA
GCTAATCAGGAAATAGATAAGTTTCAGTCTAAAGAAGATGCTCAGAAAGCTCTCCAAGACATTGAAAAT
TTTCTTGAAATGGCTCTACCCTTTATAAATTATGAACCTGAAACACTGCAGTATGAATTTGATGTAATA
TTATCTCCTGAGCTTAAGGTTCAAATGAAGACTATACAACTCAAGCTTGAAAACATTCGAAGTATATTT
GAGAACCAGCAGGCTGGTTTCAGGAACCTGGCAGATAAGCATGTGAGGCCAATCCAATTTGTGGTACCC
ACACCTGAAAATTTGGTCACATCTGGGACACCATTTTTTTCATCTAAACAAGGGAAGAAGACTTGGAGA
CAAAATCAGAGCAACTTAAAAATTGAAGTGGTGCCTGATTGTCAGGAGAAGAGAAGTTCTGGTCCATCC
TCCAGTTTGGACAATGGCAATAGCTTGGATGTTTTAAAGAACCACGTACTAAATGAACTGATACAGACT
GAGAGAGTTTATGTTCGAGAACTGTATACTGTTTTGTTGGGTTATAGAGCGGAGATGGATAATCCAGAG
ATGTTTGATCTTATGCCACCTCTCCTGAGAAATAAAAAGGACATTCTCTTTGGAAACATGGCAGAAATA
TATGAATTCCATAACGACATTTTCTTGAGCAGCCTGGAAAATTGTGCTCATGCTCCAGAAAGAGTGGGA
CCTTGTTTCCTGGAAAGGAAGGATGATTTTCAGATGTATGCAAAATATTGTCAGAATAAGCCCAGATCA
GAAACAATTTGGAGGAAGTATTCAGAATGCGCATTTTTCCAGGAATGTCAAAGAAAGTTAAAACACAGA
CTTAGACTGGATTCCTATTTACTCAAACCAGTGCAACGAATCACTAAATATCAGTTATTGTTGAAGGAG
CTATTAAAATATAGCAAAGACTGTGAAGGATCTGCTCTGTTGAAGAAGGCACTCGATGCAATGCTGGAT
TTACTGAAGTCAGTTAATGATTCTATGCATCAGATTGCAATAAATGGCTATATTGGAAACTTAAATGAA
CTGGGCAAGATGATAATGCAAGGTGGATTCAGCGTTTGGATAGGGCACAAGAAAGGTGCTACAAAAATG
AAGGATTTGGCTAGATTCAAACCAATGCAGCGACACCTTTTCTTGTATGAAAAAGCCATTGTTTTTTGC
AAAAGGCGTGTTGAAAGTGGAGAAGGCTCTGACAGATACCCGTCATACAGTTTTAAACACTGTTGGAAA
ATGGATGAAGTTGGAATCACTGAATATGTAAAAGGTGATAACCGCAAGTTTGAAATCTGGTATGGTGAA
AAGGAAGAAGTTTATATTGTCCAGGCTTCTAATGTAGATGTGAAGATGACGTGGCTAAAAGAAATAAGA
AATATTTTGTTGAAGCAGCAGGAACTTTTGACAGTTAAAAAAAGAAAGCAACAGGATCAATTAACAGAA
CGGGATAAGTTTCAGATTTCTCTTCAGCAGAATGATGAAGACCTGTGTCGGAGATGGCTCTCCTATATT
GATGAAGCTACTATGTCAAATGGCAAGTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001171878
Insert Size 2583 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001171878.1
RefSeq Size 3578 bp
RefSeq ORF 2583 bp
Locus ID 4168
UniProt ID P10911
Cytogenetics Xq27.1
Protein Families Druggable Genome
MW 100.4 kDa
Summary The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.provided by RefSeq, Jan 2010
Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence and lacks several exons compared to variant 3. The resulting isoform (e) has shorter and distinct N- and C-termini and lacks a segment compared to isoform c.
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Documents
Resources
"NM_001171878" in other vectors (6)
SKU Description Size Price
RC230549 MCF2 (Myc-DDK-tagged)-Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 5 10 ug
$787.00
RC230549L1 Lenti ORF clone of Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 5, Myc-DDK-tagged 10 ug
$1,087.00
RC230549L2 Lenti ORF clone of Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 5, mGFP tagged 10 ug
$1,087.00
RC230549L3 Lenti ORF clone of Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 5, Myc-DDK-tagged 10 ug
$1,087.00
RC230549L4 Lenti ORF clone of Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 5, mGFP tagged 10 ug
$1,087.00
RG230549 MCF2 (tGFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 5 10 ug
$987.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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