SHANK2 (NM_012309) Human Tagged ORF Clone
SKU
RC229453
SHANK2 (Myc-DDK-tagged)-Human SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant 1
$1,554.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SHANK2 |
| Synonyms | AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK; SPANK-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012309 |
| ORF Size | 5547 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012309.5 |
| RefSeq ORF | 5550 bp |
| Locus ID | 22941 |
| UniProt ID | Q9UPX8 |
| Cytogenetics | 11q13.3-q13.4 |
| MW | 201.1 kDa |
| Summary | This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density of excitatory synapses. Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, and an SH3 domain. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the postsynaptic densities of the adult and developing brain. Alterations in the encoded protein may be associated with susceptibility to autism spectrum disorder. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012309" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RG229453 | SHANK2 (tGFP-tagged) - Human SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant 1 | 10 ug |
$1,754.00
|
|
| SC303955 | SHANK2 (untagged)-Human SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant 1 | 10 ug |
$1,710.00
|
|
| SC328088 | SHANK2 (untagged)-Human SH3 and multiple ankyrin repeat domains 2 (SHANK2) transcript variant 1 | 10 ug |
$1,710.00
|
|
Citations
USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-dependent Protein Levels
Kerrisk Campbell, Sheng
J. Neurosci. (2018) 38 (23), 5289-5301 DOI: 10.1523/JNEUROSCI.3305-17.2018
Kerrisk Campbell, Sheng
J. Neurosci. (2018) 38 (23), 5289-5301 DOI: 10.1523/JNEUROSCI.3305-17.2018
Showing 1-1 of 1 papers.
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