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SHANK2 (NM_012309) Human Untagged Clone

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SKU
SC328088
SHANK2 (untagged)-Human SH3 and multiple ankyrin repeat domains 2 (SHANK2) transcript variant 1
  $1,710.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SHANK2
Synonyms AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK; SPANK-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC328088 representing NM_012309.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCCGCGCAGCCCAACATCCAGCGAGGACGAGATGGCCCAGAGCTTCTCCGACTACTCCGTGGGGTCG
GAGTCAGACAGCTCCAAAGAAGAGACCATCTATGACACGATCCGGGCCACTGCGGAGAAGCCGGGCGGT
GCCAGGACGGAGGAGAGCCAGGGCAACACGCTGGTGATCCGCGTGGTCATCCATGACCTGCAGCAGACG
AAATGCATTCGATTTAACCCGGATGCCACAGTGTGGGTTGCAAAGCAGCGGATCCTGTGTACATTAACC
CAGAGTTTGAAAGATGTCCTGAACTACGGCCTGTTCCAGCCGGCCAGCAATGGGCGTGACGGCAAGTTC
CTGGATGAGGAGCGGCTCCTGCGCGAGTACCCACAGCCCGTGGGTGAGGGCGTTCCTTCCCTGGAGTTT
CGATACAAGAAGCGGGTGTATAAACAAGCCAGTCTCGATGAGAAACAGTTGGCCAAGCTCCACACGAAG
ACCAATCTGAAGAAATGCATGGATCACATTCAGCATCGCTTGGTGGAGAAGATCACCAAGATGCTGGAC
CGAGGCCTGGATCCCAATTTCCACGACCCGGAGACCGGAGAGACCCCCCTGACCTTAGCCGCTCAGCTG
GACGACTCTGTGGAGGTCATCAAAGCTCTCAAAAATGGTGGAGCTCACCTGGACTTCCGTGCCAAAGAT
GGGATGACCGCCCTACACAAAGCTGCCCGAGCGAGGAACCAAGTTGCCCTGAAGACCCTTTTAGAGCTT
GGTGCATCCCCAGATTATAAAGACAGTTACGGCCTCACCCCGCTGTATCACACAGCCATCGTCGGAGGT
GATCCCTACTGCTGCGAGCTTCTCCTGCACGAACACGCCACTGTGTGCTGCAAAGATGAGAACGGCTGG
CACGAGATCCACCAGGCCTGCAGGTACGGGCACGTGCAGCACCTGGAGCACCTGCTGTTCTACGGGGCA
GACATGAGTGCCCAGAATGCCTCGGGGAACACGGCCTTGCACATCTGCGCCCTCTACAACCAGGACAGC
TGTGCAAGAGTGCTTCTGTTTCGAGGCGGAAATAAGGAGTTAAAAAACTACAACAGCCAGACTCCATTT
CAGGTGGCCATAATAGCAGGCAACTTTGAGCTGGCAGAATACATCAAGAACCACAAGGAAACAGACATT
GTGCCCTTCCGAGAGGCCCCGGCGTACTCCAACCGCCGGCGGCGGCCCCCCAACACGCTGGCCGCCCCC
CGGGTCCTGCTGCGCTCCAACAGTGACAACAACCTCAATGCCAGCGCTCCCGACTGGGCCGTCTGCTCC
ACGGCCACCTCGCACCGCAGCCTGTCACCCCAGCTGCTGCAGCAGATGCCCAGCAAGCCCGAGGGGGCC
GCGAAGACCATTGGGAGCTACGTGCCCGGGCCCCGCAGCCGGTCCCCATCGCTCAACAGGCTGGGCGGC
GCAGGCGAGGACGGCAAGAGGCCGCAGCCTCTCTGGCATGTCGGGTCGCCTTTTGCTCTTGGTGCCAAC
AAGGACTCACTCTCGGCCTTCGAGTACCCGGGGCCCAAGCGGAAGCTCTACAGTGCCGTGCCCGGGAGG
CTCTTCGTCGCTGTCAAGCCATACCAACCCCAAGTGGACGGCGAGATCCCCCTTCACCGCGGTGACAGG
GTCAAAGTTCTGAGCATCGGTGAAGGGGGCTTCTGGGAAGGCAGCGCCCGCGGCCACATCGGATGGTTT
CCGGCGGAGTGCGTGGAGGAGGTCCAGTGTAAGCCCAGGGACAGCCAGGCAGAAACCCGCGCTGACCGC
AGCAAGAAGCTTTTCAGGCACTACACCGTGGGCTCCTATGACAGCTTCGACACTTCCAGTGACTGCATT
ATTGAGGAGAAGACGGTGGTCCTGCAGAAAAAAGACAATGAGGGCTTTGGATTCGTGCTTCGAGGGGCC
AAAGCTGACACACCCATTGAAGAATTCACACCAACACCGGCTTTCCCAGCCCTACAGTACCTGGAGTCC
GTGGATGAAGGTGGGGTGGCGTGGCAAGCCGGACTAAGGACCGGGGACTTCTTGATTGAGGTTAACAAT
GAGAATGTTGTCAAAGTCGGCCACAGGCAGGTGGTGAACATGATCCGGCAGGGAGGGAATCACCTGGTC
CTTAAGGTGGTCACGGTGACCAGGAATCTGGACCCCGACGACACCGCCAGGAAGAAAGCTCCCCCGCCT
CCAAAGCGGGCACCGACCACAGCCCTCACCCTGCGCTCCAAGTCCATGACCTCGGAGCTGGAGGAGCTC
GTGGATAAAGCCTCGGTCCGGAAGAAGAAGGATAAACCCGAGGAGATAGTCCCGGCCTCCAAGCCCTCC
CGCGCTGCTGAGAACATGGCTGTGGAACCGAGGGTGGCGACCATCAAGCAGCGGCCCAGCAGCCGGTGC
TTCCCGGCGGGCTCAGACATGAACTCTGTGTACGAACGCCAAGGAATCGCCGTGATGACGCCCACTGTT
CCTGGGAGCCCAAAAGCCCCGTTTCTGGGCATCCCTCGAGGTACGATGCGAAGGCAGAAATCAATAGAC
AGCAGAATCTTTCTATCAGGAATAACAGAGGAAGAGCGGCAGTTTCTGGCTCCTCCAATGCTGAAGTTC
ACCAGAAGCCTGTCCATGCCGGACACCTCTGAGGACATCCCCCCTCCACCGCAGTCTGTGCCCCCGTCC
CCACCACCACCTTCCCCAACCACTTACAACTGCCCCAAGTCCCCAACTCCAAGAGTCTACGGGACGATT
AAGCCTGCGTTCAATCAGAATTCTGCCGCCAAGGTGTCCCCCGCCACCAGGTCCGACACCGTGGCCACC
ATGATGAGGGAGAAGGGGATGTACTTCAGGAGAGAGCTGGACCGCTACTCCTTGGACTCTGAAGACCTC
TACAGTCGGAATGCCGGCCCGCAAGCCAACTTCCGCAACAAGAGAGGCCAGATGCCAGAAAACCCATAC
TCAGAGGTGGGGAAGATCGCCAGCAAAGCCGTCTACGTCCCCGCCAAGCCCGCCAGGCGGAAGGGGATG
CTGGTGAAGCAGTCCAACGTGGAGGACAGCCCCGAGAAGACGTGCTCCATCCCTATCCCGACCATCATC
GTGAAGGAGCCGTCCACCAGCAGCAGCGGCAAGAGCAGCCAGGGCAGCAGCATGGAGATCGACCCCCAG
GCCCCGGAGCCACCGAGCCAGCTGCGGCCTGACGAAAGCCTGACCGTCAGCAGCCCCTTTGCCGCCGCC
ATCGCCGGAGCCGTCCGCGACCGTGAGAAGCGGCTGGAAGCCAGGAGGAACTCCCCGGCCTTCCTCTCC
ACAGACCTGGGGGATGAGGATGTGGGCCTGGGGCCACCCGCCCCCAGGACGCGGCCCTCCATGTTCCCC
GAGGAGGGGGATTTTGCTGACGAGGACAGCGCTGAGCAGCTGTCATCCCCCATGCCGAGTGCCACGCCC
AGGGAGCCCGAAAACCATTTCGTGGGTGGCGCCGAGGCCAGTGCTCCGGGTGAGGCTGGGAGGCCGCTG
AATTCCACGTCCAAAGCCCAGGGGCCCGAGAGCAGCCCAGCAGTGCCCTCCGCGAGCAGCGGCACAGCC
GGCCCCGGGAATTATGTCCACCCACTCACAGGGCGGCTGCTTGATCCCAGCTCCCCGCTGGCCCTGGCA
CTCTCCGCAAGGGACCGAGCCATGAAGGAGTCTCAACAGGGACCCAAAGGGGAGGCCCCCAAGGCCGAC
CTCAACAAACCTCTTTACATTGATACCAAAATGCGGCCCAGCCTGGATGCCGGCTTCCCTACGGTCACC
AGGCAGAACACCCGGGGACCCCTGAGGCGGCAGGAGACGGAGAACAAGTACGAGACCGACCTGGGCCGA
GACCGGAAAGGCGATGACAAGAAGAACATGCTGATCGACATCATGGACACGTCCCAGCAGAAGTCGGCT
GGCCTGCTGATGGTGCACACCGTGGACGCCACTAAGCTGGACAACGCCCTGCAGGAAGAGGACGAGAAG
GCAGAGGTGGAGATGAAGCCAGACAGCTCGCCGTCCGAGGTGCCAGAAGGTGTTTCCGAAACCGAAGGT
GCTTTACAGATCTCCGCTGCCCCCGAGCCCACCACCGTGCCCGGCAGAACCATCGTCGCGGTGGGCTCC
ATGGAAGAGGCGGTGATTTTGCCATTCCGCATCCCTCCTCCCCCTCTGGCATCCGTGGACTTGGATGAG
GATTTTATTTTTACAGAGCCATTGCCTCCTCCCCTGGAATTTGCAAATAGTTTTGATATCCCCGATGAC
CGGGCAGCTTCTGTCCCGGCTCTCTCAGACTTAGTGAAGCAGAAGAAAAGCGACACCCCTCAGTCCCCT
TCGTTGAACTCCAGCCAACCAACCAACTCTGCAGACAGCAAGAAGCCAGCCAGTCTTTCAAACTGTCTG
CCTGCCTCATTCCTGCCACCCCCTGAAAGCTTTGACGCCGTCGCCGACTCTGGGATCGAGGAGGTGGAC
AGCCGGAGTAGCAGCGACCACCACCTCGAGACGACCAGCACTATCTCCACCGTGTCTAGCATCTCCACC
CTGTCTTCCGAAGGTGGAGAGAATGTGGACACCTGCACAGTCTATGCAGATGGGCAAGCATTTATGGTT
GACAAACCCCCAGTACCTCCTAAGCCAAAAATGAAGCCCATCATTCACAAAAGCAATGCACTTTATCAA
GACGCGCTCGTGGAAGAAGATGTAGATAGCTTTGTTATCCCCCCGCCCGCTCCCCCGCCCCCGCCGGGC
AGTGCCCAGCCTGGGATGGCCAAGGTTCTCCAGCCAAGGACCTCCAAGTTGTGGGGCGACGTCACAGAG
ATCAAAAGCCCGATTCTCTCAGGCCCAAAGGCAAACGTTATTAGTGAATTGAACTCTATCCTACAGCAA
ATGAACCGAGAGAAATTGGCAAAGCCGGGGGAAGGACTGGATTCACCAATGGGAGCCAAGTCCGCCAGC
CTCGCTCCAAGAAGCCCGGAGATCATGAGCACCATCTCAGGTACACGGAGCACGACGGTCACCTTCACT
GTTCGCCCCGGCACCTCCCAGCCCATCACCCTGCAGAGCCGGCCCCCCGACTATGAAAGCAGGACCTCA
GGAACAAGACGTGCCCCAAGCCCTGTGGTCTCGCCAACAGAGATGAACAAAGAGACCCTGCCCGCCCCC
CTGTCTGCTGCCACCGCCTCTCCTTCTCCCGCTCTCTCAGATGTCTTTAGCCTTCCAAGCCAGCCCCCT
TCTGGGGATCTATTTGGCTTGAACCCAGCGGGACGCAGTAGGTCGCCATCCCCCTCGATACTGCAACAG
CCAATCTCAAATAAGCCTTTTACAACTAAACCTGTCCACCTGTGGACTAAACCAGATGTGGCCGATTGG
CTGGAAAGTCTAAACTTGGGTGAACATAAAGAGGCCTTCATGGACAATGAGATCGATGGCAGTCACTTA
CCAAACCTGCAGAAGGAGGACCTCATCGATCTTGGGGTAACTCGAGTCGGGCACAGAATGAACATAGAA
AGGGCTTTGAAACAGCTGCTGGACAGATAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_012309
Insert Size 5550 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012309.4
RefSeq Size 10675 bp
RefSeq ORF 5550 bp
Locus ID 22941
UniProt ID Q9UPX8
Cytogenetics 11q13.3-q13.4
MW 201.3 kDa
Summary This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density of excitatory synapses. Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, and an SH3 domain. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the postsynaptic densities of the adult and developing brain. Alterations in the encoded protein may be associated with susceptibility to autism spectrum disorder. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2014
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
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"NM_012309" in other vectors (3)
SKU Description Size Price
RC229453 SHANK2 (Myc-DDK-tagged)-Human SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant 1 10 ug
$1,554.00
RG229453 SHANK2 (tGFP-tagged) - Human SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant 1 10 ug
$1,754.00
SC303955 SHANK2 (untagged)-Human SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant 1 10 ug
$1,710.00

file-alt Citations

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