CSAD (NM_015989) Human Tagged ORF Clone

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SKU
RC229266
CSAD (Myc-DDK-tagged)-Human cysteine sulfinic acid decarboxylase (CSAD)
  $484.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CSAD
Synonyms CSD; PCAP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229266 representing NM_015989
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAATTCCACTGAAGTCCTCATTTCTGCTGAGCTACCTGTGCACCCTGCCTCCGGCTCTCCTGAGCA
GAGAGATCCTGATGGCTGACTCAGAAGCACTCCCCTCCCTTGCTGGGGACCCAGTGGCTGTGGAAGCCTT
GCTCCGGGCCGTGTTTGGGGTTGTTGTGGATGAGGCCATTCAGAAAGGAACCAGTGTCTCCCAGAAGGTC
TGTGAGTGGAAGGAGCCTGAGGAGCTGAAGCAGCTGCTGGATTTGGAGCTGCGGAGCCAGGGCGAGTCAC
AGAAGCAGATCCTGGAGCGGTGTCGGGCTGTGATTCGCTACAGTGTCAAGACTGGTCACCCTCGGTTCTT
CAACCAGCTCTTCTCTGGGTTGGATCCCCATGCTCTGGCCGGGCGCATTATCACTGAGAGCCTCAACACC
AGCCAGTACACATATGAAATCGCCCCCGTGTTTGTGCTCATGGAAGAGGAGGTGCTGAGGAAACTGCGGG
CCCTGGTGGGCTGGAGCTCTGGGGACGGAATCTTCTGCCCTGGTGGCTCCATCTCCAACATGTATGCTGT
AAATCTGGCCCGCTATCAGCGCTACCCGGATTGCAAGCAGAGGGGCCTCCGCACACTGCCGCCCCTGGCC
CTATTCACATCGAAGGAGTGTCACTACTCCATCCAGAAGGGAGCTGCGTTTCTGGGACTTGGCACCGACA
GTGTCCGAGTGGTCAAGGCTGATGAGAGAGGGAAAATGGTCCCCGAGGATCTGGAGAGGCAGATTGGTAT
GGCCGAGGCTGAGGGTGCTGTGCCGTTCCTGGTCAGTGCCACCTCTGGCACCACTGTGCTAGGGGCCTTT
GACCCCCTGGAGGCAATTGCTGATGTGTGCCAGCGTCATGGGCTATGGCTGCATGTGGATGCTGCCTGGG
GTGGGAGCGTCCTGCTGTCACAGACACACAGGCATCTCCTGGATGGGATCCAGAGGGCTGACTCTGTGGC
CTGGAATCCCCACAAGCTCCTCGCAGCAGGCCTGCAATGCTCTGCACTTCTTCTCCAGGATACCTCGAAC
CTGCTCAAGCGCTGCCATGGGTCCCAGGCCAGCTACCTTTTCCAGCAGGACAAGTTCTACGATGTGGCTC
TGGACACGGGAGACAAGGTGGTGCAGTGTGGCCGCCGTGTGGACTGTCTGAAGCTGTGGCTCATGTGGAA
GGCACAGGGCGATCAAGGGCTGGAGCGGCGCATCGACCAGGCCTTTGTCCTTGCCCGGTACCTGGTGGAG
GAAATGAAGAAGCGGGAAGGGTTTGAGCTAGTCATGGAGCCTGAGTTTGTCAATGTGTGTTTCTGGTTCG
TACCCCCCAGCCTGCGAGGGAAGCAGGAGAGTCCAGATTACCACGAAAGGCTGTCAAAGGTGGCCCCCGT
GCTCAAGGAGCGCATGGTGAAGGAGGGCTCCATGATGATTGGCTACCAGCCCCACGGGACCCGGGGCAAC
TTCTTCCGTGTGGTTGTGGCCAACTCTGCACTGACCTGTGCTGATATGGACTTCCTCCTCAACGAGCTGG
AGCGGCTAGGCCAGGACCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC229266 representing NM_015989
Red=Cloning site Green=Tags(s)

MSIPLKSSFLLSYLCTLPPALLSREILMADSEALPSLAGDPVAVEALLRAVFGVVVDEAIQKGTSVSQKV
CEWKEPEELKQLLDLELRSQGESQKQILERCRAVIRYSVKTGHPRFFNQLFSGLDPHALAGRIITESLNT
SQYTYEIAPVFVLMEEEVLRKLRALVGWSSGDGIFCPGGSISNMYAVNLARYQRYPDCKQRGLRTLPPLA
LFTSKECHYSIQKGAAFLGLGTDSVRVVKADERGKMVPEDLERQIGMAEAEGAVPFLVSATSGTTVLGAF
DPLEAIADVCQRHGLWLHVDAAWGGSVLLSQTHRHLLDGIQRADSVAWNPHKLLAAGLQCSALLLQDTSN
LLKRCHGSQASYLFQQDKFYDVALDTGDKVVQCGRRVDCLKLWLMWKAQGDQGLERRIDQAFVLARYLVE
EMKKREGFELVMEPEFVNVCFWFVPPSLRGKQESPDYHERLSKVAPVLKERMVKEGSMMIGYQPHGTRGN
FFRVVVANSALTCADMDFLLNELERLGQDL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015989
ORF Size 1560 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015989.5
RefSeq ORF 1563 bp
Locus ID 51380
UniProt ID Q9Y600
Cytogenetics 12q13.13
Domains pyridoxal_deC
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Taurine and hypotaurine metabolism
MW 57.8 kDa
Summary This gene encodes a member of the group 2 decarboxylase family. A similar protein in rodents plays a role in multiple biological processes as the rate-limiting enzyme in taurine biosynthesis, catalyzing the decarboxylation of cysteinesulfinate to hypotaurine. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Sep 2011
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Documents
Resources
"NM_015989" in other vectors (5)
SKU Description Size Price
RC229266L3 Lenti-ORF clone of CSAD (Myc-DDK-tagged)-Human cysteine sulfinic acid decarboxylase (CSAD) 10 ug
$784.00
RC229266L4 Lenti-ORF clone of CSAD (mGFP-tagged)-Human cysteine sulfinic acid decarboxylase (CSAD) 10 ug
$784.00
RG229266 CSAD (tGFP-tagged) - Human cysteine sulfinic acid decarboxylase (CSAD) 10 ug
$684.00
SC125291 CSAD (untagged)-Human cysteine sulfinic acid decarboxylase (CSAD) 10 ug
$457.00
SC327901 CSAD (untagged)-Human cysteine sulfinic acid decarboxylase (CSAD) 10 ug
$533.00

Plasmid Map for RC229266

Download sequence for

Cloning scheme for RC229266

Circular map for RC229266

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