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UAP1L1 (NM_207309) Human Tagged ORF Clone

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SKU
RC229260
UAP1L1 (Myc-DDK-tagged)-Human UDP-N-acteylglucosamine pyrophosphorylase 1-like 1 (UAP1L1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $472.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol UAP1L1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229260 representing NM_207309
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCGGAGCAGGACGTGCGCGCCCGGCTGCAGCGCGCTGGCCAGGAGCACCTCCTGCGCTTCTGGG
CCGAGCTGGCGCCGGAGCCACGAGCCGCGCTGCTGGCGGAGCTGGCGCTGCTGGAGCCCGAGGCGCTGCG
CGAGCACTGCCGGCGGGCGGCGGAGGCCTGCGCGCGCCCCCACGGCCCGCCGCCCGACTTGGCCGCGCGC
CTGCGGCCCCTGCCCCCAGAGCGCGTGGGCAGGGCCAGCCGCAGCGACCCCGAGACACGGCGGCGCTGGG
AGGAGGAAGGTTTCCGTCAGATTTCTCTGAACAAGGTGGCCGTCCTGCTGCTGGCTGGGGGGCAGGGCAC
TCGCCTGGGCGTGACCTACCCCAAGGGTATGTACCGTGTGGGGCTGCCCAGCCGGAAGACCCTGTACCAG
CTGCAGGCGGAGCGGATTCGGCGGGTGGAGCAGCTGGCCGGTGAGCGCCACGGGACCCGCTGCACCGTCC
CCTGGTACGTCATGACCAGCGAGTTCACTCTGGGGCCCACGGCCGAGTTCTTCAGGGAGCACAACTTCTT
CCACCTGGACCCCGCCAACGTGGTCATGTTTGAGCAGCGCCTGCTGCCTGCTGTGACCTTTGATGGCAAG
GTTATCCTGGAGCGGAAAGACAAAGTTGCCATGGCCCCAGACGGCAACGGGGGCCTCTACTGCGCGCTGG
AGGACCACAAGATCCTGGAGGACATGGAGCGCCGGGGAGTGGAGTTTGTGCACGTGTACTGTGTGGACAA
CATCCTGGTGCGGCTGGCGGACCCTGTCTTCATCGGCTTCTGTGTGTTGCAGGGCGCAGACTGTGGCGCC
AAGGTGGTGGAAAAGGCATACCCCGAGGAGCCCGTGGGCGTGGTGTGCCAGGTGGACGGTGTCCCCCAGG
TGGTGGAGTACAGCGAGATCAGTCCTGAGACCGCACAGCTACGTGCCTCCGACGGGAGCCTGCTGTACAA
TGCAGGCAACATCTGCAACCACTTCTTCACCCGAGGCTTCCTTAAGGCGGTCACCAGGGAGTTTGAGCCT
TTGCTGAAGCCACACGTGGCTGTGAAGAAGGTCCCGTATGTGGATGAGGAGGGGAATCTGGTAAAGCCGC
TAAAACCGAACGGGATAAAGATGGAGAAGTTTGTGTTTGATGTGTTCCGGTTTGCTAAGAACTTTGCTGC
CTTGGAAGTGCTGCGGGAGGAGGAATTTTCCCCACTGAAGAACGCAGAGCCAGCCGACAGGGACAGTCCC
CGCACCGCTCGCCAGGCCCTGCTCACCCAGCACTACCGGTGGGCTCTGCGGGCCGGGGCCCGCTTCCTGG
ATGCCCATGGGGCCTGGCTCCCAGAGCTGCCCAGCTTGCCCCCAAATGGAGACCCTCCGGCCATCTGTGA
GATATCGCCCTTGGTGTCTTACTCTGGAGAGGGTTTAGAAGTGTACCTGCAAGGCCGGGAGTTCCAGTCC
CCGCTCATCCTGGATGAAGACCAGGCCAGGGAGCCGCAGCTGCAGGAGTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC229260 representing NM_207309
Red=Cloning site Green=Tags(s)

MASEQDVRARLQRAGQEHLLRFWAELAPEPRAALLAELALLEPEALREHCRRAAEACARPHGPPPDLAAR
LRPLPPERVGRASRSDPETRRRWEEEGFRQISLNKVAVLLLAGGQGTRLGVTYPKGMYRVGLPSRKTLYQ
LQAERIRRVEQLAGERHGTRCTVPWYVMTSEFTLGPTAEFFREHNFFHLDPANVVMFEQRLLPAVTFDGK
VILERKDKVAMAPDGNGGLYCALEDHKILEDMERRGVEFVHVYCVDNILVRLADPVFIGFCVLQGADCGA
KVVEKAYPEEPVGVVCQVDGVPQVVEYSEISPETAQLRASDGSLLYNAGNICNHFFTRGFLKAVTREFEP
LLKPHVAVKKVPYVDEEGNLVKPLKPNGIKMEKFVFDVFRFAKNFAALEVLREEEFSPLKNAEPADRDSP
RTARQALLTQHYRWALRAGARFLDAHGAWLPELPSLPPNGDPPAICEISPLVSYSGEGLEVYLQGREFQS
PLILDEDQAREPQLQES

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_207309
ORF Size 1521 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207309.3
RefSeq ORF 1524 bp
Locus ID 91373
UniProt ID Q3KQV9
Cytogenetics 9q34.3
MW 56.8 kDa
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Documents
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"NM_207309" in other vectors (5)
SKU Description Size Price
RC229260L3 Lenti ORF clone of Human UDP-N-acteylglucosamine pyrophosphorylase 1-like 1 (UAP1L1), Myc-DDK-tagged 10 ug
$772.00
RC229260L4 Lenti ORF clone of Human UDP-N-acteylglucosamine pyrophosphorylase 1-like 1 (UAP1L1), mGFP tagged 10 ug
$772.00
RG229260 UAP1L1 (tGFP-tagged) - Human UDP-N-acteylglucosamine pyrophosphorylase 1-like 1 (UAP1L1) 10 ug
$672.00
SC308435 UAP1L1 (untagged)-Human UDP-N-acteylglucosamine pyrophosphorylase 1-like 1 (UAP1L1) 10 ug
$520.00
SC327895 UAP1L1 (untagged)-Human UDP-N-acteylglucosamine pyrophosphorylase 1-like 1 (UAP1L1) 10 ug
$520.00

Plasmid Map for RC229260

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC229260
Circular map for RC229260

file-alt Citations

Identification of UAP1L1 as a critical factor for protein O-GlcNAcylation and cell proliferation in human hepatoma cells
Lai, Liu, Tin et al
Oncogene (2018)
AI Summary: OriGene’s pUAP1L1 (Myc-DDK-tagged) (RC229260) expression vector was used in this study to overexpress human UAP1L1 protein in mammalian cells, enabling the investigation of its role in protein O-GlcNAcylation and cell proliferation in hepatoma cells.
Snippet: pUAP1L1 (Myc-DDK-tagged) (RC229260, Origene) is a mammalian expression vector that directs the synthesis of human UAP1L1 with both Myc and DDK (FLAG) tags fused in tandem to the C-terminal end of the UAP1L1 protein
Showing 1-1 of 1 papers.
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