PRPSAP1 (NM_002766) Human Tagged ORF Clone

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SKU
RC229184
PRPSAP1 (Myc-DDK-tagged)-Human phosphoribosyl pyrophosphate synthetase-associated protein 1 (PRPSAP1)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PRPSAP1
Synonyms PAP39
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229184 representing NM_002766
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAAGAAGCTGCTGCTGTTGCCCCCGCCCTCCGCGTCCTCGGCTTTCCGCGTCCCGCGCGCCCGCC
CCGTTCCCCCGCCGGCCATGAACGCCGCTCGCACCGGCTACCGAGTCTTCTCGGCCAACTCCACGGCCGC
CTGCACGGAGCTGGCCAAGCGCATCACAGAGCGCCTTGGTGCTGAATTGGGGAAGTCTGTTGTATATCAA
GAGACCAATGGAGAAACAAGAGTTGAAATAAAAGAATCTGTTCGTGGCCAAGATATTTTCATTATACAGA
CAATACCCAGAGATGTGAATACAGCTGTGATGGAGTTGCTCATCATGGCTTACGCACTGAAGACTGCCTG
TGCCAGGAACATTATTGGGGTCATCCCCTACTTCCCCTACAGCAAGCAGAGCAAGATGAGGAAGAGGGGT
TCCATTGTGTGCAAGCTGCTAGCATCCATGCTGGCGAAAGCAGGTTTAACTCACATTATCACTATGGATC
TTCATCAAAAGGAAATACAAGGCTTTTTCAGCTTTCCTGTGGACAACCTTAGAGCCTCACCTTTCCTGCT
TCAGTATATCCAGGAAGAAATTCCAAATTACAGAAATGCAGTCATTGTAGCTAAGTCTCCTGATGCTGCA
AAGAGGGCCCAGTCCTATGCGGAGAGACTGCGTCTGGGTTTGGCCGTCATTCACGGGGAAGCTCAGTGCA
CGGAACTGGACATGGACGATGGTCGTCACTCCCCGCCTATGGTCAAAAATGCTACTGTGCACCCAGGCCT
GGAGTTGCCATTGATGATGGCCAAAGAGAAGCCACCGATAACTGTAGTTGGAGATGTTGGAGGCCGCATC
GCAATCATCGTGGATGACATTATTGACGATGTGGAGAGTTTTGTTGCTGCCGCGGAGATCCTGAAAGAGA
GAGGCGCCTATAAGATCTATGTTATGGCCACCCACGGCATCCTGTCTGCAGAGGCCCCTCGCCTGATTGA
GGAGTCCTCCGTAGACGAGGTGGTGGTGACGAATACTGTCCCTCATGAGGTTCAGAAGCTGCAATGTCCC
AAGATAAAGACTGTGGATATCAGTTTGATTCTTTCTGAAGCCATTCGGAGAATCCACAATGGAGAGTCCA
TGGCCTACCTTTTCCGAAACATCACTGTGGATGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC229184 representing NM_002766
Red=Cloning site Green=Tags(s)

MPKKLLLLPPPSASSAFRVPRARPVPPPAMNAARTGYRVFSANSTAACTELAKRITERLGAELGKSVVYQ
ETNGETRVEIKESVRGQDIFIIQTIPRDVNTAVMELLIMAYALKTACARNIIGVIPYFPYSKQSKMRKRG
SIVCKLLASMLAKAGLTHIITMDLHQKEIQGFFSFPVDNLRASPFLLQYIQEEIPNYRNAVIVAKSPDAA
KRAQSYAERLRLGLAVIHGEAQCTELDMDDGRHSPPMVKNATVHPGLELPLMMAKEKPPITVVGDVGGRI
AIIVDDIIDDVESFVAAAEILKERGAYKIYVMATHGILSAEAPRLIEESSVDEVVVTNTVPHEVQKLQCP
KIKTVDISLILSEAIRRIHNGESMAYLFRNITVDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002766
ORF Size 1155 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002766.3
RefSeq ORF 1158 bp
Locus ID 5635
UniProt ID Q14558
Cytogenetics 17q25.1
Domains Pribosyltran
MW 42.3 kDa
Summary Seems to play a negative regulatory role in 5-phosphoribose 1-diphosphate synthesis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_002766" in other vectors (6)
SKU Description Size Price
RC229184L3 Lenti ORF clone of Human phosphoribosyl pyrophosphate synthetase-associated protein 1 (PRPSAP1), Myc-DDK-tagged 10 ug
$757.00
RC229184L4 Lenti ORF clone of Human phosphoribosyl pyrophosphate synthetase-associated protein 1 (PRPSAP1), mGFP tagged 10 ug
$757.00
RG229184 PRPSAP1 (tGFP-tagged) - Human phosphoribosyl pyrophosphate synthetase-associated protein 1 (PRPSAP1) 10 ug
$657.00
SC118399 PRPSAP1 (untagged)-Human phosphoribosyl pyrophosphate synthetase-associated protein 1 (PRPSAP1) 10 ug
$457.00
SC324129 PRPSAP1 (untagged)-Human phosphoribosyl pyrophosphate synthetase-associated protein 1 (PRPSAP1) 10 ug
$457.00
SC327819 PRPSAP1 (untagged)-Human phosphoribosyl pyrophosphate synthetase-associated protein 1 (PRPSAP1) 10 ug
$503.00

Plasmid Map for RC229184

Download sequence for

Cloning scheme for RC229184

Circular map for RC229184

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