BPHL (NM_004332) Human Tagged ORF Clone

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SKU
RC229142
BPHL (Myc-DDK-tagged)-Human biphenyl hydrolase-like (serine hydrolase) (BPHL), transcript variant 1
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BPHL
Synonyms BPH-RP; MCNAA; VACVASE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229142 representing NM_004332
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGCTGTGCTGGGCGGCCGGGGCGTGTTGCGCCTGCGGCTGCTTCTCTCAGCGCTGAAGCCCGGGA
TCCACGTCCCACGGGCCGGACCCGCGGCCGCGTTCGGCACCTCGGTAACCTCTGCCAAAGTGGCTGTGAA
TGGCGTTCAGCTGCATTACCAGCAGACTGGAGAGGGAGATCACGCAGTCCTGCTACTTCCTGGGATGTTA
GGAAGTGGAGAGACTGATTTTGGACCTCAGCTCAAGAACCTCAATAAGAAGCTCTTCACGGTGGTCGCCT
GGGATCCTCGAGGCTATGGACATTCCAGGCCCCCAGATCGCGATTTCCCAGCAGACTTTTTTGAAAGGGA
TGCAAAAGATGCTGTTGATTTGATGAAGGCGCTGAAGTTTAAGAAGGTTTCTCTGCTGGGGTGGAGTGAT
GGGGGCATAACCGCACTCATTGCTGCTGCAAAATATCCATCTTACATCCACAAGATGGTGATCTGGGGCG
CCAACGCCTACGTCACTGACGAAGACAGCATGATATATGAGGGCATCCGAGATGTTTCCAAATGGAGTGA
GAGAACAAGAAAGCCTCTAGAAGCCCTCTATGGGTATGACTACTTTGCCAGAACCTGTGAAAAGTGGGTG
GATGGCATAAGACAGTTTAAACATCTCCCAGATGGTAACATCTGCCGGCACCTGCTGCCCCGGGTCCAGT
GCCCCGCCTTGATTGTGCACGGTGAGAAGGATCCTCTGGTCCCACGGTTTCATGCCGACTTCATTCATAA
GCACGTGAAAGGCTCACGGCTGCATTTGATGCCAGAAGGCAAACACAACCTGCATTTGCGTTTTGCAGAT
GAATTCAACAAGTTAGCAGAAGACTTCCTACAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC229142 representing NM_004332
Red=Cloning site Green=Tags(s)

MVAVLGGRGVLRLRLLLSALKPGIHVPRAGPAAAFGTSVTSAKVAVNGVQLHYQQTGEGDHAVLLLPGML
GSGETDFGPQLKNLNKKLFTVVAWDPRGYGHSRPPDRDFPADFFERDAKDAVDLMKALKFKKVSLLGWSD
GGITALIAAAKYPSYIHKMVIWGANAYVTDEDSMIYEGIRDVSKWSERTRKPLEALYGYDYFARTCEKWV
DGIRQFKHLPDGNICRHLLPRVQCPALIVHGEKDPLVPRFHADFIHKHVKGSRLHLMPEGKHNLHLRFAD
EFNKLAEDFLQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004332
ORF Size 873 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004332.4
RefSeq ORF 876 bp
Locus ID 670
UniProt ID Q86WA6
Cytogenetics 6p25.2
Domains abhydrolase
Protein Families Druggable Genome
MW 32.4 kDa
Summary This gene encodes a member of the serine protease family of hydrolytic enzymes which contain a serine in their active site. The encoded protein may play a role in activation of the antiviral prodrug valacyclovir. Alternatively spliced transcript variants have been described.provided by RefSeq, Jan 2009
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Documents
Resources
"NM_004332" in other vectors (5)
SKU Description Size Price
RC229142L3 Lenti ORF clone of Human biphenyl hydrolase-like (serine hydrolase) (BPHL), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC229142L4 Lenti ORF clone of Human biphenyl hydrolase-like (serine hydrolase) (BPHL), transcript variant 1, mGFP tagged 10 ug
$600.00
RG229142 BPHL (tGFP-tagged) - Human biphenyl hydrolase-like (serine hydrolase) (BPHL), transcript variant 1 10 ug
$500.00
SC312761 BPHL (untagged)-Human biphenyl hydrolase-like (serine hydrolase) (BPHL), transcript variant 1 10 ug
$330.00
SC327777 BPHL (untagged)-Human biphenyl hydrolase-like (serine hydrolase) (BPHL) transcript variant 1 10 ug
$330.00

Plasmid Map for RC229142

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Cloning scheme for RC229142

Circular map for RC229142

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