KRTAP2-2 (NM_033032) Human Tagged ORF Clone

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SKU
RC229095
KRTAP2 (Myc-DDK-tagged)-Human keratin associated protein 2-2 (KRTAP2-2)
  $225.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KRTAP2-2
Synonyms KAP2.1; KAP2.2; KRTAP2-1; KRTAP2.1A; KRTAP2.1B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229095 representing NM_033032
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCGGCTCCTGCTGCGGCTCCACCTTCTCCTCCCTGAGCTACGGGGGAGGCTGCTGCCAGCCCTGCT
GCTGCCGCGACCCCTGCTGCTGCCGCCCCGTGACCTGCCAGACCACCGTGTGCCGCCCCGTGACCTGCGT
GCCCCGCTGCACGCGCCCCATCTGCGAGCCCTGCCGCCGCCCGGTGTGCTGCGACCCCTGCTCCCTGCAG
GAAGGCTGCTGCCGCCCCATCACCTGCTGCCCCTCGTCGTGCACGGCTGTGGTGTGCAGGCCCTGCTGCT
GGGCCACCACCTGCTGCCAGCCTGTGTCTGTGCAGTCCCCCTGCGGCCAGCCGACCCCTTGCAGCACCAC
CTGCAGGACCTCCTCCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC229095 representing NM_033032
Red=Cloning site Green=Tags(s)

MTGSCCGSTFSSLSYGGGCCQPCCCRDPCCCRPVTCQTTVCRPVTCVPRCTRPICEPCRRPVCCDPCSLQ
EGCCRPITCCPSSCTAVVCRPCCWATTCCQPVSVQSPCGQPTPCSTTCRTSSC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033032
ORF Size 369 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033032.2, NP_149021.2
RefSeq ORF 372 bp
Locus ID 728279
UniProt ID Q9BYT5
Cytogenetics 17q21.2
MW 12.8 kDa
Summary In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high-sulfur and high-glycine-tyrosine keratins (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_033032" in other vectors (5)
SKU Description Size Price
RC229095L3 Lenti ORF clone of Human keratin associated protein 2-2 (KRTAP2-2), Myc-DDK-tagged 10 ug
$525.00
RC229095L4 Lenti ORF clone of Human keratin associated protein 2-2 (KRTAP2-2), mGFP tagged 10 ug
$525.00
RG229095 KRTAP2 (tGFP-tagged) - Human keratin associated protein 2-2 (KRTAP2-2) 10 ug
$425.00
SC324723 KRTAP2 (untagged)-Human keratin associated protein 2-2 (KRTAP2-2) 10 ug
$165.00
SC327730 KRTAP2 (untagged)-Human keratin associated protein 2-2 (KRTAP2-2) 10 ug
$165.00

Plasmid Map for RC229095

Download sequence for

Cloning scheme for RC229095

Circular map for RC229095

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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