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HTR3D (NM_001163646) Human Tagged ORF Clone

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SKU
RC228368
HTR3D (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 3 family member D (HTR3D), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HTR3D
Synonyms 5HT3D
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC228368 representing NM_001163646
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAAACACTCTCCAGGCCCCCCAGCCCTGGCCCTCCTCTCTCAGTCTCTGCTCACTACAGGAAATG
GCGACACTTTGATTATCAATTGCCCAGGCTTTGGCCAGCACAGGGTGGACCCTGCAGCCTTTCAAGCAGT
GTTTGACAGAAAGGCTATTGGTCCAGTCACCAACTACAGTGTCGCCACTCATGTCAACATCTCCTTCACC
TTGTCTGCCATCTGGAACTGCTATTCCAGGATTCACACCTTCAACTGCCACCATGCCCGGCCTTGGCACA
ATCAATTTGTGCAGTGGAACCCAGATGAATGCGGAGGCATCAAGAAGTCCGGCATGGCAACTGAGAACCT
ATGGCTTTCAGATGTCTTCATCGAGGAGTCTGTGGATCAGACACCTGCAGGTCTCATGGCTAGTATGTCA
ATAGTGAAGGCCACATCAAACACAATAAGCCAATGTGGGTGGTCAGCATCTGCAAACTGGACACCTTCTA
TTTCCCCTTCCATGGACAGAGCCCGGGCATGGAGAAGGATGTCCAGGAGCTTTCAAATACATCACAGAAC
CTCATTCAGAACAAGGAGGGAGTGGGTACTGCTGGGTATCCAAAAAAGAACAATAAAGGTGACCGTGGCC
ACTAACCAGTATGAACAAGCCATCTTCCATGTGGCCATCAGGCGCAGGTGCAGGCCCAGCCCCTACGTGG
TAAACTTTCTGGTGCCCAGTGGCATTCTGATTGCCATCGATGCCCTCAGTTTCTACCTGCCACTGGAAAG
TGGGAATTGTGCCCCATTCAAGATGACTGTTCTGCTGGGCTACAGCGTCTTCCTGCTCATGATGAATGAC
TTGCTCCCAGCCACTAGCACTTCATCACATGCTTCACTAGTGGCGCCTCTGGCCCTCATGCAGACCCCCT
TGCCTGCAGGTGTCTACTTCGCCCTGTGCCTGTCCCTGATGGTGGGCAGCCTGCTGGAGACCATCTTCAT
CACCCACCTGCTGCACGTGGCCACCACCCAGCCCCTACCTCTGCCTCGGTGGCTCCACTCCCTGCTGCTG
CACTGCACCGGCCAAGGGAGATGCTGTCCCACTGCGCCCCAGAAGGGAAATAAGGGCCCGGGTCTCACCC
CCACCCACCTGCCCGGTGTGAAGGAGCCAGAGGTATCAGCAGGGCAGATGCCAGGCCCTGGGGAGGCAGA
GCTGACAGGGGGCTCAGAATGGACAAGGGCCCAGCGGGAACACGAGGCCCAGAAGCAGCACTCGGTGGAG
CTGTGGGTGCAGTTCAGCCACGCGATGGACGCCCTGCTCTTCCGCCTCTACCTGCTCTTCATGGCCTCCT
CCATCATCACCGTCATATGCCTCTGGAACACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC228368 representing NM_001163646
Red=Cloning site Green=Tags(s)

MQKHSPGPPALALLSQSLLTTGNGDTLIINCPGFGQHRVDPAAFQAVFDRKAIGPVTNYSVATHVNISFT
LSAIWNCYSRIHTFNCHHARPWHNQFVQWNPDECGGIKKSGMATENLWLSDVFIEESVDQTPAGLMASMS
IVKATSNTISQCGWSASANWTPSISPSMDRARAWRRMSRSFQIHHRTSFRTRREWVLLGIQKRTIKVTVA
TNQYEQAIFHVAIRRRCRPSPYVVNFLVPSGILIAIDALSFYLPLESGNCAPFKMTVLLGYSVFLLMMND
LLPATSTSSHASLVAPLALMQTPLPAGVYFALCLSLMVGSLLETIFITHLLHVATTQPLPLPRWLHSLLL
HCTGQGRCCPTAPQKGNKGPGLTPTHLPGVKEPEVSAGQMPGPGEAELTGGSEWTRAQREHEAQKQHSVE
LWVQFSHAMDALLFRLYLLFMASSIITVICLWNT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001163646
ORF Size 1362 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163646.2
RefSeq ORF 1365 bp
Locus ID 200909
UniProt ID Q70Z44
Cytogenetics 3q27.1
Protein Families Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
MW 50 kDa
Summary The protein encoded this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit D of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a mitogen and a hormone. This hormone has been linked to neuropsychiatric disorders, including anxiety, depression, and migraine. Serotonin receptors causes fast and depolarizing responses in neurons following activation. The genes encoding subunits C, D and E of this type 3 receptor form a cluster on chromosome 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2009
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Documents
Resources
"NM_001163646" in other vectors (4)
SKU Description Size Price
RC228368L3 Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 3 family member D (HTR3D), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC228368L4 Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 3 family member D (HTR3D), transcript variant 3, mGFP tagged 10 ug
$757.00
RG228368 HTR3D (tGFP-tagged) - Human 5-hydroxytryptamine (serotonin) receptor 3 family member D (HTR3D), transcript variant 3 10 ug
$657.00
SC327003 HTR3D (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 3 family member D (HTR3D) transcript variant 3 10 ug
$457.00

Plasmid Map for RC228368

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC228368
Circular map for RC228368

file-alt Citations

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