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HTR3D (NM_001163646) Human Untagged Clone

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SKU
SC327003
HTR3D (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 3 family member D (HTR3D) transcript variant 3
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol HTR3D
Synonyms 5HT3D
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC327003 representing NM_001163646.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCAGAAACACTCTCCAGGCCCCCCAGCCCTGGCCCTCCTCTCTCAGTCTCTGCTCACTACAGGAAAT
GGCGACACTTTGATTATCAATTGCCCAGGCTTTGGCCAGCACAGGGTGGACCCTGCAGCCTTTCAAGCA
GTGTTTGACAGAAAGGCTATTGGTCCAGTCACCAACTACAGTGTCGCCACTCATGTCAACATCTCCTTC
ACCTTGTCTGCCATCTGGAACTGCTATTCCAGGATTCACACCTTCAACTGCCACCATGCCCGGCCTTGG
CACAATCAATTTGTGCAGTGGAACCCAGATGAATGCGGAGGCATCAAGAAGTCCGGCATGGCAACTGAG
AACCTATGGCTTTCAGATGTCTTCATCGAGGAGTCTGTGGATCAGACACCTGCAGGTCTCATGGCTAGT
ATGTCAATAGTGAAGGCCACATCAAACACAATAAGCCAATGTGGGTGGTCAGCATCTGCAAACTGGACA
CCTTCTATTTCCCCTTCCATGGACAGAGCCCGGGCATGGAGAAGGATGTCCAGGAGCTTTCAAATACAT
CACAGAACCTCATTCAGAACAAGGAGGGAGTGGGTACTGCTGGGTATCCAAAAAAGAACAATAAAGGTG
ACCGTGGCCACTAACCAGTATGAACAAGCCATCTTCCATGTGGCCATCAGGCGCAGGTGCAGGCCCAGC
CCCTACGTGGTAAACTTTCTGGTGCCCAGTGGCATTCTGATTGCCATCGATGCCCTCAGTTTCTACCTG
CCACTGGAAAGTGGGAATTGTGCCCCATTCAAGATGACTGTTCTGCTGGGCTACAGCGTCTTCCTGCTC
ATGATGAATGACTTGCTCCCAGCCACTAGCACTTCATCACATGCTTCACTAGTGGCGCCTCTGGCCCTC
ATGCAGACCCCCTTGCCTGCAGGTGTCTACTTCGCCCTGTGCCTGTCCCTGATGGTGGGCAGCCTGCTG
GAGACCATCTTCATCACCCACCTGCTGCACGTGGCCACCACCCAGCCCCTACCTCTGCCTCGGTGGCTC
CACTCCCTGCTGCTGCACTGCACCGGCCAAGGGAGATGCTGTCCCACTGCGCCCCAGAAGGGAAATAAG
GGCCCGGGTCTCACCCCCACCCACCTGCCCGGTGTGAAGGAGCCAGAGGTATCAGCAGGGCAGATGCCA
GGCCCTGGGGAGGCAGAGCTGACAGGGGGCTCAGAATGGACAAGGGCCCAGCGGGAACACGAGGCCCAG
AAGCAGCACTCGGTGGAGCTGTGGGTGCAGTTCAGCCACGCGATGGACGCCCTGCTCTTCCGCCTCTAC
CTGCTCTTCATGGCCTCCTCCATCATCACCGTCATATGCCTCTGGAACACCTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001163646
Insert Size 1365 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163646.1
RefSeq Size 1786 bp
RefSeq ORF 1365 bp
Locus ID 200909
UniProt ID Q70Z44
Cytogenetics 3q27.1
Protein Families Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
MW 50.2 kDa
Summary The protein encoded this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit D of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a mitogen and a hormone. This hormone has been linked to neuropsychiatric disorders, including anxiety, depression, and migraine. Serotonin receptors causes fast and depolarizing responses in neurons following activation. The genes encoding subunits C, D and E of this type 3 receptor form a cluster on chromosome 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2009
Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_001163646" in other vectors (4)
SKU Description Size Price
RC228368 HTR3D (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 3 family member D (HTR3D), transcript variant 3 10 ug
$457.00
RC228368L3 Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 3 family member D (HTR3D), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC228368L4 Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 3 family member D (HTR3D), transcript variant 3, mGFP tagged 10 ug
$757.00
RG228368 HTR3D (tGFP-tagged) - Human 5-hydroxytryptamine (serotonin) receptor 3 family member D (HTR3D), transcript variant 3 10 ug
$657.00

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