STEAP1B (NM_001164460) Human Tagged ORF Clone

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SKU
RC228274
STEAP1B (Myc-DDK-tagged)-Human STEAP family member 1B (STEAP1B), transcript variant 1
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol STEAP1B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC228274 representing NM_001164460
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAGCAGAAAAGACATCACAAACCAAGAAGAAATTTGGAAAATGAAGCCTAGGAGAAATTTAGAAG
ACAACGATTATTTGCATGAGGACACGGGAGAGACCAGCATGCTAAAAAGACCTGTGCTTTTGCATTTGCA
GCAAACAGCCCATGCTGATGAATTTGACTGCCCTTCAGAACTTCAGCACGCACAGGAACTCTTTCCACAG
TGGCACTTGCCAATTAAAATAGCTGCTGTTATGGCATCTCTGACTTTTCTTTACACTCTTCTGAGGGAAG
TAATTCACCCTTTAGCAACTTCCCATCAACAATATTTTTATAAAATTCCAATCCTGGTCATCAACAAAGT
CTTGCCAATGGTTTCCATCACTCTCTTGGCATTGGTTTACCTACCAGGTGTGATAGCAGCAATTGTCCAA
GTTCATAATGGAACCAAGTATAAGAAGTTTCCACATTGGTTGGATAAGTGGATGTTAACAAGAAAGCAGT
TTGGGCTTCTCAGTTTGTTTTTTGCTGTACTGCATGCAATTTATACTCTGTCTTACGCAATGAGGCGATC
CTACAGATACAAGTTGCTAAACTGGGCATATCAACAGGTCCAACAAAATAAAGAAGATGCCTGGATTGAG
CATGATGTTTGGAGAATGGAGATTTATGTGTCTCTGGGAATTGTGGGACTGGCAATACTGGCTCTGTTGG
CTGTGACATCTATTCCATCTGTGAGTGACTCTTTGACATGGAGAGAATTTCACTATATTCAGGCTATACT
GACTGGAAGCCTGGTAGCTTTGTGCAACTTTCAGGCCACTAAACTGGCCAAGACACAACTGGTAGGCACA
CCAAATTTCTGGCAGGAGCAGCAGTGTGGAATCCATTTCCCACCACCTTCCAAGACTAGGAGTTATATAT
ACTTCATTTTATCAGCAGCCAGCCCTTCAAAGGGAAATGTTAGCTCTCACATGTTTCAGCTTCATCTAGA
AAATCTCAATAAAAAAGACAAGTTATTGCTGATCATCTTTCAGATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC228274 representing NM_001164460
Red=Cloning site Green=Tags(s)

MESRKDITNQEEIWKMKPRRNLEDNDYLHEDTGETSMLKRPVLLHLQQTAHADEFDCPSELQHAQELFPQ
WHLPIKIAAVMASLTFLYTLLREVIHPLATSHQQYFYKIPILVINKVLPMVSITLLALVYLPGVIAAIVQ
VHNGTKYKKFPHWLDKWMLTRKQFGLLSLFFAVLHAIYTLSYAMRRSYRYKLLNWAYQQVQQNKEDAWIE
HDVWRMEIYVSLGIVGLAILALLAVTSIPSVSDSLTWREFHYIQAILTGSLVALCNFQATKLAKTQLVGT
PNFWQEQQCGIHFPPPSKTRSYIYFILSAASPSKGNVSSHMFQLHLENLNKKDKLLLIIFQI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001164460
ORF Size 1026 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001164460.2
RefSeq ORF 1029 bp
Locus ID 256227
UniProt ID Q6NZ63
Cytogenetics 7p15.3
Protein Families Transmembrane
MW 39.5 kDa
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Documents
Resources
"NM_001164460" in other vectors (4)
SKU Description Size Price
RC228274L3 Lenti ORF clone of Human STEAP family member 1B (STEAP1B), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC228274L4 Lenti ORF clone of Human STEAP family member 1B (STEAP1B), transcript variant 1, mGFP tagged 10 ug
$757.00
RG228274 STEAP1B (tGFP-tagged) - Human STEAP family member 1B (STEAP1B), transcript variant 1 10 ug
$657.00
SC326909 STEAP1B (untagged)-Human STEAP family protein MGC87042 (MGC87042) transcript variant 1 10 ug
$503.00

Plasmid Map for RC228274

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Cloning scheme for RC228274

Circular map for RC228274

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